Methotrexate-d₃ diammonium

Cat. No.: HY-14519S1: FAQs
Data Sheet Handling Instructions

Molarity Calculator

Methotrexate-d₃ diammonium is the deuterium labeled Methotrexate diammonium. Methotrexate (Amethopterin), an antimetabolite and antifolate agent, inhibits the enzyme dihydrofolate reductase, thereby preventing the conversion of folic acid into tetrahydrofolate, and inhibiting DNA synthesis. Methotrexate, also an immunosuppressant and antineoplastic agent, is used for the research of rheumatoid arthritis and a number of different cancers (such as acute lymphoblastic leukemia).

For research use only. We do not sell to patients.

Methotrexate-d<sub>3</sub> diammonium Chemical Structure

Methotrexate-d₃ diammonium Chemical Structure

Size	Price	Stock	Quantity
5 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 190	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 380	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 610	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 980	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Methotrexate-d₃ diammonium:

Methotrexate-d₃ In-stock

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Molecular Weight

491.52

Formula

C₂₀H₂₅D₃N₁₀O₅

Appearance

Solid

Color

Yellow to brown

SMILES

NC1=NC(N)=NC2=C1N=C(CN(C3=CC=C(C(N[C@H](C(O)=O)CCC(O)=O)=O)C=C3)C([2H])([2H])[2H])C=N2.N.N

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (101.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0345 mL	10.1725 mL	20.3451 mL
5 mM	0.4069 mL	2.0345 mL	4.0690 mL
10 mM	0.2035 mL	1.0173 mL	2.0345 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Tian H, et al. Understanding the mechanisms of action of methotrexate: implications for the treatment of rheumatoid arthritis. Bull NYU Hosp Jt Dis. 2007;65(3):168-73. [Content Brief]

[2]. Swierkot J, et al. Methotrexate in rheumatoid arthritis. Pharmacol Rep. 2006 Jul-Aug;58(4):473-92. [Content Brief]

[3]. Ehab Tousson, et al. The Effect of L-carnitine on Amethopterin-induced Toxicity in Rat Large Intestine. Journal of Cancer Research and Treatment, 2014 2 (3), pp 55-63.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0345 mL	10.1725 mL	20.3451 mL	50.8626 mL
	5 mM	0.4069 mL	2.0345 mL	4.0690 mL	10.1725 mL
	10 mM	0.2035 mL	1.0173 mL	2.0345 mL	5.0863 mL
	15 mM	0.1356 mL	0.6782 mL	1.3563 mL	3.3908 mL
	20 mM	0.1017 mL	0.5086 mL	1.0173 mL	2.5431 mL
	25 mM	0.0814 mL	0.4069 mL	0.8138 mL	2.0345 mL
	30 mM	0.0678 mL	0.3391 mL	0.6782 mL	1.6954 mL
	40 mM	0.0509 mL	0.2543 mL	0.5086 mL	1.2716 mL
	50 mM	0.0407 mL	0.2035 mL	0.4069 mL	1.0173 mL
	60 mM	0.0339 mL	0.1695 mL	0.3391 mL	0.8477 mL
	80 mM	0.0254 mL	0.1272 mL	0.2543 mL	0.6358 mL
	100 mM	0.0203 mL	0.1017 mL	0.2035 mL	0.5086 mL