Methotrexate-d3 diammonium
Based on 1 Customer Validation
Methotrexate-d3 diammonium is the deuterated-labeled Methotrexate (HY-14519). Methotrexate (Amethopterin; CL14377; WR19039) is an orally active antifolate (Antifolate). Methotrexate inhibits dihydrofolate reductase (DHFR), blocks tetrahydrofolate production, suppresses purine/pyrimidine synthesis and transmethylation, and causes intracellular accumulation of AICAR. Methotrexate promotes extracellular adenosine release, regulates the cytokine network, and inhibits the alarmin function of HMGB1. Methotrexate induces apoptosis and cytotoxicity, upregulates the expression of iNOS and COX-2, suppresses hippocampal neurogenesis, and induces pulmonary fibrosis. Methotrexate is used in the research of various immune and inflammation-related diseases such as arthritis, psoriasis, systemic lupus erythematosus, as well as pulmonary fibrosis and breast cancer.
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- Purity : 99.69%
- 화학식: C20H25D3N10O5
- 분자량:491.52
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보관:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
IC50 & Target
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COX-2 |
IL-8 |
iNOS |
신청
1. This compound can be used as a tracer
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
Chemical Information
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Unlabeled CAS 59-05-2
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Appearance Solid
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분자량 491.52
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화학식 C20H25D3N10O5
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Color Yellow to brown
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SMILES
NC1=NC(N)=NC2=C1N=C(CN(C3=CC=C(C(N[C@H](C(O)=O)CCC(O)=O)=O)C=C3)C([2H])([2H])[2H])C=N2.N.N
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선적
Room temperature in continental US; may vary elsewhere.
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보관
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
용액&용해도
In Vitro:
DMSO : 50 mg/mL (101.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Cronstein BN, et al. The mechanism of action of methotrexate. Rheumatic diseases clinics of North America. 1997 Nov;23(4):739-55. [Content Brief]
[2]. Bedoui Y, et al. Methotrexate an Old Drug with New Tricks. International journal of molecular sciences. 2019 Oct 10;20(20):5023. [Content Brief]
[4]. Ohbayashi M, et al. Induction of pulmonary fibrosis by methotrexate treatment in mice lung in vivo and in vitro. The Journal of toxicological sciences. 2010 Oct;35(5):653-61. [Content Brief]
[5]. Yang M, et al. Acute treatment with methotrexate induces hippocampal dysfunction in a mouse model of breast cancer. Brain research bulletin. 2012 Oct 01;89(1-2):50-6. [Content Brief]
[6]. Banji D, et al. Evaluation of the concomitant use of methotrexate and curcumin on Freund's complete adjuvant-induced arthritis and hematological indices in rats. Indian J Pharmacol. 2011 Sep;43(5):546-50. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0345 mL | 10.1725 mL | 20.3451 mL | 50.8626 mL |
| 5 mM | 0.4069 mL | 2.0345 mL | 4.0690 mL | 10.1725 mL | |
| 10 mM | 0.2035 mL | 1.0173 mL | 2.0345 mL | 5.0863 mL | |
| 15 mM | 0.1356 mL | 0.6782 mL | 1.3563 mL | 3.3908 mL | |
| 20 mM | 0.1017 mL | 0.5086 mL | 1.0173 mL | 2.5431 mL | |
| 25 mM | 0.0814 mL | 0.4069 mL | 0.8138 mL | 2.0345 mL | |
| 30 mM | 0.0678 mL | 0.3391 mL | 0.6782 mL | 1.6954 mL | |
| 40 mM | 0.0509 mL | 0.2543 mL | 0.5086 mL | 1.2716 mL | |
| 50 mM | 0.0407 mL | 0.2035 mL | 0.4069 mL | 1.0173 mL | |
| 60 mM | 0.0339 mL | 0.1695 mL | 0.3391 mL | 0.8477 mL | |
| 80 mM | 0.0254 mL | 0.1272 mL | 0.2543 mL | 0.6358 mL | |
| 100 mM | 0.0203 mL | 0.1017 mL | 0.2035 mL | 0.5086 mL |
Methotrexate-d3 diammonium Related Classifications
HY-14519S1 Related Classifications
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Keywords
- Methotrexate-d3 diammonium
- Isotope-Labeled Compounds
- Antifolate
- Dihydrofolate reductase (DHFR)
- NO Synthase
- COX
- Interleukin Related
- tetrahydrofolate
- AICAR transformylase
- ROS
- COX-2
- iNOS
- HMGB1 alarmin
- purine/pyrimidine synthesis
- dihydrofolate reductase
- methotrexate-polyglutamates
- leukotriene B4
- Inhibitor
- inhibitor
- inhibit