Myristoyllysophosphatidylcholine
Myristoyllysophosphatidylcholine is a NLRP3 inflammasome inhibitor with multiple activities including anti-inflammatory and antioxidant effects. Myristoyllysophosphatidylcholine inhibits LPS-induced NLRP3 inflammasome activation, reduces pro-inflammatory cytokine secretion, decreases ROS and MDA levels, alleviates lung tissue and cell damage, and mediates apoptosis. Myristoyllysophosphatidylcholine serves as a myristate donor for myristoylation of the Glucose-6-Phosphate Isomerase (GPI) anchor in Trypanosoma brucei and undergoes hydrolysis. Myristoyllysophosphatidylcholine acts as a biomarker for the severity of community-acquired pneumonia. Myristoyllysophosphatidylcholine can be used in research related to diseases such as community-acquired pneumonia and acute lung injury.
For research use only. We do not sell to patients.
- CAS No.: 13699-45-1
- Formula: C22H46NO7P
- Molecular Weight:467.58
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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NLRP3 |
IL-1β |
IL-6 |
Caspase-1 |
TNF-α |
Myristoyllysophosphatidylcholine (LPC 14:0) (10-40 μM; 1 h pretreatment, 12 h LPS exposure) shows no toxicity to RAW 264.7 cells at concentrations up to 20 μM, while higher concentrations combined with LPS reduce cell viability[1].
Myristoyllysophosphatidylcholine (20 μM; 1 h pretreatment, 12 h LPS exposure) significantly reduces LPS-induced apoptosis and ROS production in RAW 264.7 cells; it significantly attenuates the depletion of SOD and GSH; and it significantly inhibits the secretion of IL-1β, IL-6, and TNF-α[1].
Myristoyllysophosphatidylcholine (10-20 μM; 1 h pretreatment, 12 h LPS exposure) significantly inhibits LPS-induced activation of the NLRP3 inflammasome in RAW 264.7 cells, including reducing the expression of NLRP3, TXNIP, Caspase-1 and IL-1β[1].
Myristoyllysophosphatidylcholine (M-LPC) radiolabeled probe [3H]myristoyllysophosphatidylcholine (78 nM; 0-40 min) labels free GPI lipids in cultured Trypanosoma brucei MITat 1.2 variant 221 at an identical rate to [3H]myristate, undergoes rapid hydrolysis to extracellular free myristic acid, and labels phosphatidylcholine in serum-free medium[2].
Myristoyllysophosphatidylcholine (2.5 µM) inhibits VSG myristoylation mediated by 360 nM [3H]myristic acid by 30% in cultured Trypanosoma brucei MITat 1.2 strain 221 variant[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RAW 264.7 cells
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Concentration:10, 20, 30, 40 μM
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Incubation Time:1 h (pretreatment); 12 h (LPS exposure)
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Result:Exhibited no toxicity to RAW 264.7 cells at concentrations of 10, 20, 30, and 40 μM when administered alone.
Significantly reduced cell viability at concentrations of 30 and 40 μM when combined with 1 μg/mL LPS, while 10 and 20 μM showed no significant difference from the control.
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Cell Line:RAW 264.7 cells
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Concentration:20 μM
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Incubation Time:1 h (pretreatment); 12 h (LPS exposure)
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Result:Reduced the LPS-induced apoptosis ratio of RAW 264.7 cells.
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Cell Line:RAW 264.7 cells
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Concentration:20 μM
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Incubation Time:1 h (pretreatment); 12 h (LPS exposure)
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Result:Reduced the secretion of IL-1β, IL-6, and TNF-α.
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Cell Line:RAW 264.7 cells
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Concentration:10, 20 μM
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Incubation Time:1 h (pretreatment); 12 h (LPS exposure)
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Result:Reduced LPS-induced activation of the NLRP3 inflammasome, including reduced expression of NLRP3, TXNIP, Caspase-1, and IL-1β, in RAW 264.7 cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 (8-week-old male, SPF-grade, LPS-induced ALI)[1]
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Dosage:10 mg/kg
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Administration:s.c.; 2 doses (2 hours before LPS instillation, 10 hours after LPS instillation)
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Result:Cut lung injury score, lung W/D ratio, BALF total protein, BALF total cell count, lung MPO activity, BALF IL-1β and TNF-α, as well as lung MDA content.
Elevated lung GSH concentration and SOD specific activity.
Suppressed LPS-triggered upregulation of NLRP3, TXNIP, Caspase-1 and IL-1β protein in lung tissue.
Chemical Information
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CAS No. 13699-45-1
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Molecular Weight 467.58
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Formula C22H46NO7P
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SMILES
O=C(OCC(O)COP(=O)([O-])OCC[N+](C)(C)C)CCCCCCCCCCCCC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Nan W, et al. Myristoyl lysophosphatidylcholine is a biomarker and potential therapeutic target for community-acquired pneumonia. Redox biology. 2022 Dec;58:102556. [Content Brief]
[2]. Werbovetz KA, et al. Lipid metabolism in Trypanosoma brucei: utilization of myristate and myristoyllysophosphatidylcholine for myristoylation of glycosyl phosphatidylinositols. The Biochemical journal. 1996 Sep 01;318 ( Pt 2)(Pt 2):575-81. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Myristoyllysophosphatidylcholine Related Classifications
HY-165316 Related Classifications
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- Myristoyllysophosphatidylcholine
- 13699-45-1
- NOD-like Receptor (NLR)
- Interleukin Related
- Reactive Oxygen Species (ROS)
- Caspase
- TNF Receptor
- Apoptosis
- NLRP3 inflammasome
- RAW 264.7 cells
- IL-1β
- GPI anchor
- VSG
- nagana
- african sleeping sickness
- community-acquired pneumonia
- Trypanosoma brucei
- acute lung injury
- Inhibitor
- inhibitor
- inhibit