Myxothiazol
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Myxothiazol, an antifungal antibiotic, is a mitochondrial electron transport chain complex III (bc1 complex) inhibitor. Myxothiazol inhibits the growth of many yeasts and fungi at concentrations between 0.01 and 3 μg/ml.
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- Purity: 99.98%
- CAS No.: 76706-55-3
- 화학식: C25H33N3O3S2
- 분자량:487.68
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보관:
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
0.01 ng/mL
Compound: Myxothiazol
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Cytotoxicity against human A549 cells after 72 hrs by SRB assay
Cytotoxicity against human A549 cells after 72 hrs by SRB assay
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[PMID: 10096868] |
| HCT-15 | IC50 |
0.03 ng/mL
Compound: Myxothiazol
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Cytotoxicity against human HCT-15 cells after 72 hrs by SRB assay
Cytotoxicity against human HCT-15 cells after 72 hrs by SRB assay
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[PMID: 10096868] |
| SK-MEL-2 | IC50 |
9.7 ng/mL
Compound: Myxothiazol
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Cytotoxicity against human SK-MEL-2 cells after 72 hrs by SRB assay
Cytotoxicity against human SK-MEL-2 cells after 72 hrs by SRB assay
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[PMID: 10096868] |
| SK-OV-3 | IC50 |
0.01 ng/mL
Compound: Myxothiazol
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Cytotoxicity against human SKOV3 cells after 72 hrs by SRB assay
Cytotoxicity against human SKOV3 cells after 72 hrs by SRB assay
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[PMID: 10096868] |
| XF498 | IC50 |
0.03 ng/mL
Compound: Myxothiazol
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Cytotoxicity against human XF498 cells after 72 hrs by SRB assay
Cytotoxicity against human XF498 cells after 72 hrs by SRB assay
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[PMID: 10096868] |
Myxothiazol inhibits the growth of many yeasts and fungi at concentrations between 0.01 and 3 μg/ml[2].
Myxothiazol binds to the ubiquinol oxidation site Qo of complex III and blocks electron transfer from ubiquinol to cytochrome b and thus inhibits complex III activity[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57Bl/J6 mice[3]
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Dosage:0.56 mg/kg
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Administration:I.p.; 24 hours intervals for at most 4 times
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Result:A reversible complex III activity decrease to 50% of control value occurred at 2 h post-injection. At 74 h only minor histological changes in the liver were found, supercomplex formation was preserved and no significant changes in the expression of genes indicating hepatotoxicity or inflammation were found.
Chemical Information
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CAS No. 76706-55-3
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Appearance Solid
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분자량 487.68
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화학식 C25H33N3O3S2
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Color White to off-white
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SMILES
C[C@H](C1=NC(C2=NC(/C=C/[C@H](OC)[C@@H](C)/C(OC)=C\C(N)=O)=CS2)=CS1)/C=C/C=C/C(C)C
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Structure Classification
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Initial Source
Myxococcus fulvus
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선적
Room temperature in continental US; may vary elsewhere.
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보관
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
용액&용해도
DMSO : ≥ 50 mg/mL (102.53 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.56 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (271 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
References
[1]. Thierbach G, et al. Myxothiazol, a new antibiotic interfering with respiration. Antimicrob Agents Chemother. 1981;19(4):504-507. [Content Brief]
[2]. von Jagow G,et al. An inhibitor of mitochondrial respiration which binds to cytochrome b and displaces quinone from the iron-sulfur protein of the cytochrome bc1 complex. J Biol Chem. 1984;259(10):6318-6326. [Content Brief]
[3]. Davoudi M, et al. A mouse model of mitochondrial complex III dysfunction induced by myxothiazol. Biochem Biophys Res Commun. 2014;446(4):1079-1084. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0505 mL | 10.2526 mL | 20.5052 mL | 51.2631 mL |
| 5 mM | 0.4101 mL | 2.0505 mL | 4.1010 mL | 10.2526 mL | |
| 10 mM | 0.2051 mL | 1.0253 mL | 2.0505 mL | 5.1263 mL | |
| 15 mM | 0.1367 mL | 0.6835 mL | 1.3670 mL | 3.4175 mL | |
| 20 mM | 0.1025 mL | 0.5126 mL | 1.0253 mL | 2.5632 mL | |
| 25 mM | 0.0820 mL | 0.4101 mL | 0.8202 mL | 2.0505 mL | |
| 30 mM | 0.0684 mL | 0.3418 mL | 0.6835 mL | 1.7088 mL | |
| 40 mM | 0.0513 mL | 0.2563 mL | 0.5126 mL | 1.2816 mL | |
| 50 mM | 0.0410 mL | 0.2051 mL | 0.4101 mL | 1.0253 mL | |
| 60 mM | 0.0342 mL | 0.1709 mL | 0.3418 mL | 0.8544 mL | |
| 80 mM | 0.0256 mL | 0.1282 mL | 0.2563 mL | 0.6408 mL | |
| 100 mM | 0.0205 mL | 0.1025 mL | 0.2051 mL | 0.5126 mL |
Myxothiazol Related Classifications
HY-112177 Related Classifications
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