Cat. No.: HY-117348

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NCC-149

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NCC-149 is a selective HDAC8 inhibitor and can be used for neural differentiation research.

For research use only. We do not sell to patients.

Purity: 98.65%
CAS No.: 1316652-41-1
Formula: C₁₆H₁₄N₄O₂S
Molecular Weight:326.37

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

NCC-149 Chemical Structure

CAS No.: 1316652-41-1

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

NCC-149 is a selective HDAC8 inhibitor and can be used for neural differentiation research^[1].

IC₅₀ & Target

HDAC8

All HDAC Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	54 μM Compound: C149	Inhibition of HDAC in human HeLa cells nuclear extracts by fluorometric assay Inhibition of HDAC in human HeLa cells nuclear extracts by fluorometric assay	[PMID: 23116147]
HH	GI₅₀	21 μM Compound: 16; NCC149	Antiproliferative activity against human HH cells assessed as cell growth inhibition measured after 72 hrs by CCK-8 assay Antiproliferative activity against human HH cells assessed as cell growth inhibition measured after 72 hrs by CCK-8 assay	[PMID: 37429084]
HH	GI₅₀	23 μM Compound: C149	Growth inhibition of human HH cells incubated for 72 hrs by MTS assay Growth inhibition of human HH cells incubated for 72 hrs by MTS assay	[PMID: 23116147]
HuT78	GI₅₀	32 μM Compound: 16; NCC149	Anti-proliferative activity against human HuT78 cells assessed as growth inhibition incubated for 72 hrs by alamar blue staining based fluorometric assay Anti-proliferative activity against human HuT78 cells assessed as growth inhibition incubated for 72 hrs by alamar blue staining based fluorometric assay	[PMID: 37429084]
Jurkat	GI₅₀	2.8 μM Compound: 16; NCC149	Antiproliferative activity against human Jurkat cells assessed as cell growth inhibition measured after 72 hrs by CCK-8 assay Antiproliferative activity against human Jurkat cells assessed as cell growth inhibition measured after 72 hrs by CCK-8 assay	[PMID: 37429084]
Jurkat	GI₅₀	7.6 μM Compound: C149	Growth inhibition of human Jurkat cells incubated for 72 hrs by MTS assay Growth inhibition of human Jurkat cells incubated for 72 hrs by MTS assay	[PMID: 23116147]
MT2	GI₅₀	11 μM Compound: C149	Growth inhibition of human MT2 cells incubated for 72 hrs by MTS assay Growth inhibition of human MT2 cells incubated for 72 hrs by MTS assay	[PMID: 23116147]
MT4	GI₅₀	15 μM Compound: C149	Growth inhibition of human MT4 cells incubated for 72 hrs by MTS assay Growth inhibition of human MT4 cells incubated for 72 hrs by MTS assay	[PMID: 23116147]
MT4	GI₅₀	22 μM Compound: 16; NCC149	Cytotoxicity against human MT4 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay Cytotoxicity against human MT4 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay	[PMID: 37429084]
NB1	GI₅₀	12 μM Compound: C149	Growth inhibition of human NB-1 cells incubated for 72 hrs by MTS assay Growth inhibition of human NB-1 cells incubated for 72 hrs by MTS assay	[PMID: 23116147]
PBMC	GI₅₀	>100 μM Compound: C149	Growth inhibition of human PBMC PB-N cells incubated for 72 hrs by MTS assay Growth inhibition of human PBMC PB-N cells incubated for 72 hrs by MTS assay	[PMID: 23116147]
PBMC	GI₅₀	>100 μM Compound: C149	Growth inhibition of human PBMC PB-O cells incubated for 72 hrs by MTS assay Growth inhibition of human PBMC PB-O cells incubated for 72 hrs by MTS assay	[PMID: 23116147]

In Vitro

NCC-149 (5 μM; 3 days) remarkably reduces NeuN expression levels in P19 cells^[1].
NCC-149 (0-40 μM; 4 days) reduces embryoid body size in a dose-dependent manner^[1].
NCC-149 (5 and 20 μM; 24 h) downregulates P19 cell proliferation^[1].
NCC-149 (2.5 and 5 μM; 24 h) leads to cell growth retardation by G2/M phase arrest^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

P19 cells
Concentration:

5 μM
Incubation Time:

3 days
Result:

Remarkably reduced NeuN expression levels.

Cell Proliferation Assay^[1]

Cell Line:

P19 cells
Concentration:

5 and 20 μM
Incubation Time:

24 h
Result:

Downregulated cell proliferation.

Cell Cycle Analysis^[1]

Cell Line:

P19 cells
Concentration:

2.5 and 5 μM
Incubation Time:

24 h
Result:

Led to a significant increase in G2/M phase cells, with a slight decrease in S phase cells.

RT-PCR^[1]

Cell Line:

P19 cells
Concentration:

25 μM or 2.5 and 5 μM
Incubation Time:

4 days (25 μM) or 48 h (2.5 and 5 μM)
Result:

Did not reduce the HDAC8 expression at the mRNA level.
Significantly and partially reduced cyclin B1 and cyclin A2 gene expression, respectively.

Chemical Information

CAS No. 1316652-41-1
Appearance Solid
Molecular Weight 326.37
Formula C₁₆H₁₄N₄O₂S
Color Off-white to light yellow
SMILES

O=C(NO)C1=CC=CC(C2=CN(CSC3=CC=CC=C3)N=N2)=C1
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (383.00 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.0640 mL

15.3200 mL

30.6401 mL

5 mM

0.6128 mL

3.0640 mL

6.1280 mL

10 mM

0.3064 mL

1.5320 mL

3.0640 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Katayama S, et al. HDAC8 regulates neural differentiation through embryoid body formation in P19 cells. Biochem Biophys Res Commun. 2018 Mar 25;498(1):45-51. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.0640 mL	15.3200 mL	30.6401 mL	76.6002 mL
	5 mM	0.6128 mL	3.0640 mL	6.1280 mL	15.3200 mL
	10 mM	0.3064 mL	1.5320 mL	3.0640 mL	7.6600 mL
	15 mM	0.2043 mL	1.0213 mL	2.0427 mL	5.1067 mL
	20 mM	0.1532 mL	0.7660 mL	1.5320 mL	3.8300 mL
	25 mM	0.1226 mL	0.6128 mL	1.2256 mL	3.0640 mL
	30 mM	0.1021 mL	0.5107 mL	1.0213 mL	2.5533 mL
	40 mM	0.0766 mL	0.3830 mL	0.7660 mL	1.9150 mL
	50 mM	0.0613 mL	0.3064 mL	0.6128 mL	1.5320 mL
	60 mM	0.0511 mL	0.2553 mL	0.5107 mL	1.2767 mL
	80 mM	0.0383 mL	0.1915 mL	0.3830 mL	0.9575 mL
	100 mM	0.0306 mL	0.1532 mL	0.3064 mL	0.7660 mL

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NCC-149

Reprogramming Compound Library

Oxygen Sensing Compound Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Liver Cancer Compound Library

Osteogenesis Compound Library

Highly Selective Inhibitors Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Brain Cancer Compound Library

High-Throughput Bioactive Compound Library

High-Efficiency Gene Editing Compound Library

Lactylation Compound Library

MCE Kinase Hinge Binder Fragment Library

Cell Cycle/DNA Damage Compound Library

Epigenetics Compound Library

Histone Modification Research Compound Library

Anti-Aging Compound Library

All HDAC Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

NCC-149

Reprogramming Compound Library

Oxygen Sensing Compound Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Liver Cancer Compound Library

Osteogenesis Compound Library

Highly Selective Inhibitors Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Brain Cancer Compound Library

High-Throughput Bioactive Compound Library

High-Efficiency Gene Editing Compound Library

Lactylation Compound Library

MCE Kinase Hinge Binder Fragment Library

Cell Cycle/DNA Damage Compound Library

Epigenetics Compound Library

Histone Modification Research Compound Library

Anti-Aging Compound Library

All HDAC Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

NCC-149 Related Classifications

HY-117348 Related Classifications