2. Cell Cycle/DNA Damage Epigenetics
  3. HDAC
  4. NCC-149

NCC-149 is a selective HDAC8 inhibitor and can be used for neural differentiation research.

For research use only. We do not sell to patients.

NCC-149 Chemical Structure

NCC-149 Chemical Structure

CAS No. : 1316652-41-1

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Solid + Solvent
10 mM * 1 mL in DMSO
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5 mg USD 310 In-stock
10 mg USD 480 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

NCC-149 Related Antibodies

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Description

NCC-149 is a selective HDAC8 inhibitor and can be used for neural differentiation research[1].

IC50 & Target

HDAC8

 

In Vitro

NCC-149 (5 μM; 3 days) remarkably reduces NeuN expression levels in P19 cells[1].
NCC-149 (0-40 μM; 4 days) reduces embryoid body size in a dose-dependent manner[1].
NCC-149 (5 and 20 μM; 24 h) downregulates P19 cell proliferation[1].
NCC-149 (2.5 and 5 μM; 24 h) leads to cell growth retardation by G2/M phase arrest[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NCC-149 Related Antibodies

Western Blot Analysis[1]

Cell Line: P19 cells
Concentration: 5 μM
Incubation Time: 3 days
Result: Remarkably reduced NeuN expression levels.

Cell Proliferation Assay[1]

Cell Line: P19 cells
Concentration: 5 and 20 μM
Incubation Time: 24 h
Result: Downregulated cell proliferation.

Cell Cycle Analysis[1]

Cell Line: P19 cells
Concentration: 2.5 and 5 μM
Incubation Time: 24 h
Result: Led to a significant increase in G2/M phase cells, with a slight decrease in S phase cells.

RT-PCR[1]

Cell Line: P19 cells
Concentration: 25 μM or 2.5 and 5 μM
Incubation Time: 4 days (25 μM) or 48 h (2.5 and 5 μM)
Result: Did not reduce the HDAC8 expression at the mRNA level.
Significantly and partially reduced cyclin B1 and cyclin A2 gene expression, respectively.
Molecular Weight

326.37

Formula

C16H14N4O2S
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(NO)C1=CC=CC(C2=CN(CSC3=CC=CC=C3)N=N2)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (383.00 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0640 mL 15.3200 mL 30.6401 mL
5 mM 0.6128 mL 3.0640 mL 6.1280 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.14%

COA (245 KB) RP-HPLC (244 KB) LCMS (112 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0640 mL 15.3200 mL 30.6401 mL 76.6002 mL
5 mM 0.6128 mL 3.0640 mL 6.1280 mL 15.3200 mL
10 mM 0.3064 mL 1.5320 mL 3.0640 mL 7.6600 mL
15 mM 0.2043 mL 1.0213 mL 2.0427 mL 5.1067 mL
20 mM 0.1532 mL 0.7660 mL 1.5320 mL 3.8300 mL
25 mM 0.1226 mL 0.6128 mL 1.2256 mL 3.0640 mL
30 mM 0.1021 mL 0.5107 mL 1.0213 mL 2.5533 mL
40 mM 0.0766 mL 0.3830 mL 0.7660 mL 1.9150 mL
50 mM 0.0613 mL 0.3064 mL 0.6128 mL 1.5320 mL
60 mM 0.0511 mL 0.2553 mL 0.5107 mL 1.2767 mL
80 mM 0.0383 mL 0.1915 mL 0.3830 mL 0.9575 mL
100 mM 0.0306 mL 0.1532 mL 0.3064 mL 0.7660 mL
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NCC-149 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NCC-1491316652-41-1NCC149NCC 149HDACHistone deacetylasesembryoid bodyNeuNP19neural differentiationInhibitorinhibitorinhibit

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