Nitrocefin
Based on 2 publication(s) in Google Scholar
Nitrocefin is a highly activated, chromogenic cephalosporin derivative. Nitrocefin is a chromogenic β-lactamase substrate. Nitrocefin undergoes a distinctive color change from yellow to red as the amide bond in the β-lactam ring is hydrolyzed by β-lactamase. Nitrocefin is used in competitive inhibition studies in developmental work on β-lactamase-resistant antibiotics.
For research use only. We do not sell to patients.
- Purity: 98.92%
- CAS No.: 41906-86-9
- Formula: C21H16N4O8S2
- Molecular Weight:516.50
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) Nitrocefin
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Customer Validation & Images
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Bio/Physico-chemical Assay
All Beta-lactamase Isoforms
MoreAll Antibiotic Isoforms
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Biological Activity
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β-lactam |
Nitrocefin is hydrolysised by β-Lactamase from Mycobacterium tuberculosis[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 41906-86-9
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Appearance Solid
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Molecular Weight 516.50
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Formula C21H16N4O8S2
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Color Yellow to orange
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SMILES
O=C(C(N12)=C(/C=C/C3=CC=C([N+]([O-])=O)C=C3[N+]([O-])=O)CS[C@]2([H])[C@H](NC(CC4=CC=CS4)=O)C1=O)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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mBio
Deficiency in peptidoglycan recycling promotes β-lactam sensitivity in Caulobacter crescentus. [Abstract]2025 Apr 9;16(4):e0297524. PMID: 40066998
Nitrocefin purchased from MedChemExpress. Usage Cited in: mBio. 2025 Apr 9;16(4):e0297524. [Abstract]
Cell lysates of WT, RP47 (∆sdpA), and RP56 (∆ampG) with and without ampicillin treatment were incubated with Nitrocefin solution (1 mg/mL) for 30 min. M2 buffer was used as a negative control. The appearance of red color product indicates a positive β-lactamase activity, while a consistent yellow color indicates the absence of activity.
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Solvent & Solubility
DMSO : 100 mg/mL (193.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (467 KB)
- English - EN (467 KB)
- Français - FR (467 KB)
- Deutsch - DE (467 KB)
- Norwegian - NO (467 KB)
- Español - ES (467 KB)
- Swedish - SV (467 KB)
- Italian - IT (467 KB)
- Korean - KR (467 KB)
- Portuguese - PT (467 KB)
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Handling Instructions (2659 KB)
References
[1]. Lee M, et al. A practical synthesis of nitrocefin. J Org Chem. 2005 Jan 7;70(1):367-9. [Content Brief]
[2]. Worthington RJ, et al. Overcoming resistance to β-lactam antibiotics. J Org Chem. 2013 May 3;78(9):4207-13. [Content Brief]
[3]. O'Callaghan CH, et al. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972 Apr;1(4):283-8. [Content Brief]
[4]. Chow C, et al. Blanchard JS. Kinetic characterization of hydrolysis of nitrocefin, cefoxitin, and meropenem by β-lactamase from Mycobacterium tuberculosis. Biochemistry. 2013 Jun 11;52(23):4097-104. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.9361 mL | 9.6805 mL | 19.3611 mL | 48.4027 mL |
| 5 mM | 0.3872 mL | 1.9361 mL | 3.8722 mL | 9.6805 mL | |
| 10 mM | 0.1936 mL | 0.9681 mL | 1.9361 mL | 4.8403 mL | |
| 15 mM | 0.1291 mL | 0.6454 mL | 1.2907 mL | 3.2268 mL | |
| 20 mM | 0.0968 mL | 0.4840 mL | 0.9681 mL | 2.4201 mL | |
| 25 mM | 0.0774 mL | 0.3872 mL | 0.7744 mL | 1.9361 mL | |
| 30 mM | 0.0645 mL | 0.3227 mL | 0.6454 mL | 1.6134 mL | |
| 40 mM | 0.0484 mL | 0.2420 mL | 0.4840 mL | 1.2101 mL | |
| 50 mM | 0.0387 mL | 0.1936 mL | 0.3872 mL | 0.9681 mL | |
| 60 mM | 0.0323 mL | 0.1613 mL | 0.3227 mL | 0.8067 mL | |
| 80 mM | 0.0242 mL | 0.1210 mL | 0.2420 mL | 0.6050 mL | |
| 100 mM | 0.0194 mL | 0.0968 mL | 0.1936 mL | 0.4840 mL |
Nitrocefin Related Classifications
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