Cat. No.: HY-N6057

Obtusin

Name: Obtusin
Brand: MedChemExpress
SKU: HY-N6057
Price: 50.00 USD
Availability: InStock
Rating: 5 (1 reviews)

고객리뷰

Based on 1 Customer Validation

Obtusin is an orally active human monoamine oxidase A (hMAO-A) inhibitor discovered from the seeds of Cassia obtusifolia, with a K_i value of 6.15 μM. Obtusin inhibits high glucose-induced activation of the MAPKs/NF-κB/VEGF pathway and downregulates the expression of Poldip2, Nox4, VCAM-1, and HIF-1α, thereby alleviating oxidative stress, endothelial activation, and angiogenesis-related functions. Obtusin ameliorates diabetic retinopathy in vivo. Obtusin is used in research related to diabetes and neurodegenerative diseases (anxiety and depression).

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity : 99.10%
CAS No.: 70588-05-5
화학식: C₁₈H₁₆O₇
분자량:344.32

보관:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Obtusin Chemical Structure

CAS No.: 70588-05-5

사이즈

가격

재고

수량

Amount: USD 0.00

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
용액&용해도
- In Vitro
순도&문서
References
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

IC₅₀ & Target

^[2]^[1]

hMAO-A

11.12 μM (IC₅₀)

hMAO-A

6.15 μM (Ki)

HIF-1α

All Monoamine Oxidase Isoforms :

MAO-A
MAO-B

All p38 MAPK Isoforms :

All NF-κB Isoforms :

All VEGFR Isoforms :

All NADPH Oxidase Isoforms :

All HIF/HIF Prolyl-Hydroxylase Isoforms :

In Vitro

Oxidative StressLocal Vascular RegulationOthers

Oxidative Stress

Local Vascular Regulation

Obtusin (10-20 μM; 48 h) alleviates high glucose-induced HRMEC dysfunction, oxidative stress, and endothelial activation by inhibiting proliferation, migration, and tube formation, and downregulating the expression of Poldip2, Nox4, VCAM-1, and HIF-1α^[1].

Local Vascular Regulation

Obtusin (10-20 μM; 2 h) inhibits the activation of high glucose-induced MAPKs/NF-κB/VEGF pathway in HRMECs and decreases the expression of phosphorylated p38 MAPK, phosphorylated NF-κB p65, and VEGFA^[1].

Others

Obtusin selectively inhibits hMAO-A activity with an IC₅₀ of 11.12 μM^[2].
Obtusin competitively inhibits hMAO-A with a K_i value of 6.15 μM^[2].
Obtusin binds to the catalytic site of hMAO-A with a binding energy of -8.51 kcal/mol, forming key hydrogen bond interactions with Cys323 and Thr336^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

HRMECs
Concentration:

10 and 20 μM
Incubation Time:

48 h
Result:

Determined 10 μM and 20 μM obtusin for 48 h as optimal conditions.
Dose-dependently suppressed proliferation and migration.
Inhibited tube-forming ability dose-dependently.

Western Blot Analysis^[1]

Cell Line:

HRMECs
Concentration:

10 and 20 μM
Incubation Time:

2 h
Result:

Significantly inhibited VEGFA mRNA expression.
Markedly reduced intracellular ROS levels.
Effectively reversed changes in Poldip2, Nox4, VCAM-1, and HIF-1α protein expression.
Reversed changes in MAPKs/NF-κB/VEGF pathway proteins (p-p38, p-p65, VEGFA).

In Vivo

Innate ImmunityOxidative Stress

Innate Immunity

Oxidative Stress

Obtusin (5-10 mg/kg/day; p.o.; daily; 12 weeks) ameliorates diabetic retinopathy in db/db mice by improving retinal histopathology, reducing oxidative stress, and inhibiting inflammation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BLKS/J Lepr db mutant mice (seven-week-old, male)^[1]
Dosage:

5 and 10 mg/kg/day
Administration:

p.o.; daily; 12 weeks
Result:

Reduced blood glucose levels from weeks 12 to 20 and improved body weight by week 14.
Corrected abnormal lipid profiles by reducing serum TC, TG, and LDL levels at 20 weeks.
Reduced GCL edema, increased cell numbers in the INL and ONL, and improved retinal thickness and morphology.
Reduced vascular leaks, improved retinal vascular permeability, and diminished overall fluorescence intensity.
Significantly reduced pericyte loss and the formation of acellular capillaries.
Reduced ROS levels across all retinal layers.
Alleviated structural abnormalities in retinal ganglion cells and vascular endothelial cells in a dose-dependent manner.
Reduced glial cell activation (IBA1 and GFAP) and preserved ganglion cell numbers (BRN3A) in a dose-dependent manner.
Significantly reduced the elevated levels of iNOS, IL-1β, IL-6, and VEGFA in the retinas.

Related Protocols :

Chemical Information

CAS No. 70588-05-5
Appearance Solid
분자량 344.32
화학식 C₁₈H₁₆O₇
Color Yellow to orange
SMILES

O=C1C2=C(C=C(C)C(O)=C2OC)C(C3=CC(OC)=C(OC)C(O)=C13)=O
Structure Classification
- Quinones
- Anthraquinones
- Phenols
- Polyphenols
Initial Source
- Plant
- Other Families Extract
선적

Room temperature in continental US; may vary elsewhere.
보관

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

용액&용해도

In Vitro:

DMSO : 5 mg/mL (14.52 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.9043 mL

14.5214 mL

29.0428 mL

5 mM

0.5809 mL

2.9043 mL

5.8085 mL

10 mM

0.2904 mL

1.4521 mL

2.9043 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (291 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Xu J, et al. Obtusin ameliorates diabetic retinopathy by inhibiting oxidative stress and inflammation. Psychopharmacology. 2024 Dec;241(12):2471-2484.

[2]. Paudel P, et al. In Vitro and in Silico Human Monoamine Oxidase Inhibitory Potential of Anthraquinones, Naphthopyrones, and Naphthalenic Lactones from Cassia obtusifolia Linn Seeds. ACS Omega. 2019 Sep 18;4(14):16139-16152. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.9043 mL	14.5214 mL	29.0428 mL	72.6069 mL
	5 mM	0.5809 mL	2.9043 mL	5.8086 mL	14.5214 mL
	10 mM	0.2904 mL	1.4521 mL	2.9043 mL	7.2607 mL

상품명:
Cat. No.:
수량:
MCE Japan Authorized Agent:

상품명:
CAS No.:
Species:
Purity:
수량:

Obtusin

All Monoamine Oxidase Isoforms

All p38 MAPK Isoforms

All NF-κB Isoforms

All VEGFR Isoforms

All NADPH Oxidase Isoforms

All HIF/HIF Prolyl-Hydroxylase Isoforms