ODN TTAGGG  (Synonyms: ODN A151)

ODN TTAGGG (ODN A151), an inhibitory oligonucleotide (ODN), is a TLR9, AIM2, and cGAS antagonist. ODN TTAGGG inhibits AIM2 inflammasome activation and cGAS activation through competition with DNA. ODN TTAGGG blocks AIM2-mediated Pyroptosis and inhibits IL-18 production. ODN TTAGGG has immunosuppressive effects and can be used in research on related autoimmune diseases such as lupus erythematosus^[1][2][3]. ODN TTAGGG sequence: 5'-T-T-A-G-G-G-T-T-A-G-G-G-T-T-A-G-G-G-T-T-A-G-G-G-3'.

ODN TTAGGG reduces IFNβ responses to both viruses (HSV-1 or MCMV) in BMDC and BMDM^[1].
ODN TTAGGG suppresses IL-18 secretion in response to poly(dA:dT) but not nigericin in BMDC^[1].
ODN TTAGGG (6 µg) is capable of interacting with AIM2 even in the absence of an activating stimulus^[1].
ODN TTAGGG blocks inflammasome assembly in an AIM2-citrine reporter cell line^[1].
ODN TTAGGG (0.3 μM) blocks cGAS-mediated type I IFN response induced by cytosolic DNA in THP-1 cells^[2].
ODN TTAGGG (0.3-3 μM; 7 days) inhibits autonomous type I IFN response in TREX1 deficient THP-1 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ODN TTAGGG (50 µg; instilled into the lungs) significantly reduces the pulmonary inflammation induced by mechanical ventilation in mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

7944.30

1801724-76-4

Solid

White to off-white

DNA, d(P-thio)(T-T-A-G-G-G-T-T-A-G-G-G-T-T-A-G-G-G-T-T-A-G-G-G)

[DNA, d(P-thio)(T-T-A-G-G-G-T-T-A-G-G-G-T-T-A-G-G-G-T-T-A-G-G-G)]

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. John J Kaminski, et al. Synthetic oligodeoxynucleotides containing suppressive TTAGGG motifs inhibit AIM2 inflammasome activation. J Immunol. 2013 Oct 1;191(7):3876-83.  [Content Brief]

  [2]. Folkert Steinhagen, et al. Suppressive oligodeoxynucleotides containing TTAGGG motifs inhibit cGAS activation in human monocytes. Eur J Immunol. 2018 Apr;48(4):605-611.  [Content Brief]

  [3]. Scheiermann J, et al. Suppressive oligonucleotides inhibit inflammation in a murine model of mechanical ventilator induced lung injury. J Thorac Dis. 2016 Sep;8(9):2434-2443.  [Content Brief]