PI3K/Akt/mTOR-IN-4
PI3K/Akt/mTOR-IN-4 (compound 4r) is a potent PI3K/Akt/mTOR and tubulin polymerization inhibitor. PI3K/Akt/mTOR-IN-4 induce apoptosis and cell cycle arrest at G2/M phase. PI3K/Akt/mTOR-IN-4 decreases the expression of p-PI3K, p-Akt, and p-mTOR, β-tubulin.
For research use only. We do not sell to patients.
- Formula: C29H29FN4O3
- Molecular Weight:500.56
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Ca-Ski | IC50 |
7.24 μM
Compound: 4r
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Antiproliferative activity against human Ca-Ski cells assessed as cell viability incubated for 48 hrs by MTT assay
Antiproliferative activity against human Ca-Ski cells assessed as cell viability incubated for 48 hrs by MTT assay
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[PMID: 38636129] |
| HEK-293T | IC50 |
23.96 μM
Compound: 4r
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Cytotoxicity against human HEK293T cells assessed as cell viability incubated for 48 hrs by MTT assay
Cytotoxicity against human HEK293T cells assessed as cell viability incubated for 48 hrs by MTT assay
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[PMID: 38636129] |
| HeLa | IC50 |
5.03 μM
Compound: 4r
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Antiproliferative activity against human HeLa cells assessed as cell viability incubated for 48 hrs by MTT assay
Antiproliferative activity against human HeLa cells assessed as cell viability incubated for 48 hrs by MTT assay
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[PMID: 38636129] |
| L02 | IC50 |
21.08 μM
Compound: 4r
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Cytotoxicity against human L02 cells assessed as cell viability incubated for 48 hrs by MTT assay
Cytotoxicity against human L02 cells assessed as cell viability incubated for 48 hrs by MTT assay
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[PMID: 38636129] |
| SiHa | IC50 |
3.38 μM
Compound: 4r
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Antiproliferative activity against human SiHa cells assessed as cell viability incubated for 48 hrs by MTT assay
Antiproliferative activity against human SiHa cells assessed as cell viability incubated for 48 hrs by MTT assay
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[PMID: 38636129] |
PI3K/Akt/mTOR-IN-4 (compound 4r) (0-100 µM; 48 h) shows antiproliferative activity with IC50s of 3.38, 5.03, 7.24, 21.08, 23.96 µM for SiHa, HeLa, Ca Ski, LO2, HEK-293t cells, respectively[1].
PI3K/Akt/mTOR-IN-4 (0-16 µM; 24 h) induces apoptosis and cell cycle arrest at G2/M phase[1].
PI3K/Akt/mTOR-IN-4 (4, 8,16 µM; 24 h) decreases the expression of phosphorylation of PI3K, Akt, mTOR level and β-tubulin protein in a dose-dependent manner[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:SiHa, HeLa, Ca Ski, LO2, HEK-293t cells
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Concentration:0-100 µM
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Incubation Time:48 h
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Result:Inhibited cell proliferative with IC50s of 3.38, 5.03, 7.24, 21.08, 23.96 µM for SiHa, HeLa, Ca Ski, LO2, HEK-293t cells, respectively.
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Cell Line:SiHa cells
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Concentration:0-16 µM
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Incubation Time:24 h
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Result:Induced cell cycle arrest at G2/M phase with G2/M phase cells accumulating from 5.24 % (Ctrl) to 28.37 % (16 μM).
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Cell Line:SiHa cells
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Concentration:0-16 µM
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Incubation Time:24 h
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Result:Induced apoptosis the percentage of apoptotic cells were increased from 11.62 % (Ctrl) to 98.56 % (16 μM).
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Cell Line:SiHa cells
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Concentration:4, 8, 16 μM
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Incubation Time:24 h
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Result:Decreased the expression of phosphorylation of PI3K, Akt, and mTOR, β-tubulin in a dose-dependent manner.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:zebrafish embryos[1]
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Dosage:0, 12.5, 25, 50, 100, 200, 400 μM
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Administration:0-96 h
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Result:Showed no toxicity for zebrafish embryos.
Chemical Information
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Molecular Weight 500.56
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Formula C29H29FN4O3
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SMILES
COC1=C(OC)C(OC)=CC(C2=NC3=CC(N4CCN(C)CC4)=C(C#CC5=CC=CC=C5F)C=C3N2)=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
PI3K/Akt/mTOR-IN-4 Related Classifications
HY-163511 Related Classifications
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