2. PI3K/Akt/mTOR Apoptosis Cytoskeleton Cell Cycle/DNA Damage
  3. Akt Apoptosis mTOR PI3K Microtubule/Tubulin
  4. PI3K/Akt/mTOR-IN-4

PI3K/Akt/mTOR-IN-4 (compound 4r) is a potent PI3K/Akt/mTOR and tubulin polymerization inhibitor. PI3K/Akt/mTOR-IN-4 induce apoptosis and cell cycle arrest at G2/M phase. PI3K/Akt/mTOR-IN-4 decreases the expression of p-PI3K, p-Akt, and p-mTOR, β-tubulin.

For research use only. We do not sell to patients.

PI3K/Akt/mTOR-IN-4

PI3K/Akt/mTOR-IN-4 Chemical Structure

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

PI3K/Akt/mTOR-IN-4 Related Antibodies

Top Publications Citing Use of Products

View All Akt Isoform Specific Products:

View All Isoforms
Akt Akt1 Akt2 Akt3

View All mTOR Isoform Specific Products:

View All Isoforms
mTOR mTORC1 mTORC2

View All PI3K Isoform Specific Products:

View All Isoforms
PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

PI3K/Akt/mTOR-IN-4 (compound 4r) is a potent PI3K/Akt/mTOR and tubulin polymerization inhibitor. PI3K/Akt/mTOR-IN-4 induce apoptosis and cell cycle arrest at G2/M phase. PI3K/Akt/mTOR-IN-4 decreases the expression of p-PI3K, p-Akt, and p-mTOR, β-tubulin[1].

Cellular Effect
Cell Line Type Value Description References
Ca-Ski IC50
7.24 μM
Compound: 4r
Antiproliferative activity against human Ca-Ski cells assessed as cell viability incubated for 48 hrs by MTT assay
Antiproliferative activity against human Ca-Ski cells assessed as cell viability incubated for 48 hrs by MTT assay
[PMID: 38636129]
HEK-293T IC50
23.96 μM
Compound: 4r
Cytotoxicity against human HEK293T cells assessed as cell viability incubated for 48 hrs by MTT assay
Cytotoxicity against human HEK293T cells assessed as cell viability incubated for 48 hrs by MTT assay
[PMID: 38636129]
HeLa IC50
5.03 μM
Compound: 4r
Antiproliferative activity against human HeLa cells assessed as cell viability incubated for 48 hrs by MTT assay
Antiproliferative activity against human HeLa cells assessed as cell viability incubated for 48 hrs by MTT assay
[PMID: 38636129]
L02 IC50
21.08 μM
Compound: 4r
Cytotoxicity against human L02 cells assessed as cell viability incubated for 48 hrs by MTT assay
Cytotoxicity against human L02 cells assessed as cell viability incubated for 48 hrs by MTT assay
[PMID: 38636129]
SiHa IC50
3.38 μM
Compound: 4r
Antiproliferative activity against human SiHa cells assessed as cell viability incubated for 48 hrs by MTT assay
Antiproliferative activity against human SiHa cells assessed as cell viability incubated for 48 hrs by MTT assay
[PMID: 38636129]
In Vitro

PI3K/Akt/mTOR-IN-4 (compound 4r) (0-100 µM; 48 h) shows antiproliferative activity with IC50s of 3.38, 5.03, 7.24, 21.08, 23.96 µM for SiHa, HeLa, Ca Ski, LO2, HEK-293t cells, respectively[1].
PI3K/Akt/mTOR-IN-4 (0-16 µM; 24 h) induces apoptosis and cell cycle arrest at G2/M phase[1].
PI3K/Akt/mTOR-IN-4 (4, 8,16 µM; 24 h) decreases the expression of phosphorylation of PI3K, Akt, mTOR level and β-tubulin protein in a dose-dependent manner[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PI3K/Akt/mTOR-IN-4 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: SiHa, HeLa, Ca Ski, LO2, HEK-293t cells
Concentration: 0-100 µM
Incubation Time: 48 h
Result: Inhibited cell proliferative with IC50s of 3.38, 5.03, 7.24, 21.08, 23.96 µM for SiHa, HeLa, Ca Ski, LO2, HEK-293t cells, respectively.

Cell Cycle Analysis[1]

Cell Line: SiHa cells
Concentration: 0-16 µM
Incubation Time: 24 h
Result: Induced cell cycle arrest at G2/M phase with G2/M phase cells accumulating from 5.24 % (Ctrl) to 28.37 % (16 μM).

Apoptosis Analysis[1]

Cell Line: SiHa cells
Concentration: 0-16 µM
Incubation Time: 24 h
Result: Induced apoptosis the percentage of apoptotic cells were increased from 11.62 % (Ctrl) to 98.56 % (16 μM).

Western Blot Analysis[1]

Cell Line: SiHa cells
Concentration: 4, 8, 16 μM
Incubation Time: 24 h
Result: Decreased the expression of phosphorylation of PI3K, Akt, and mTOR, β-tubulin in a dose-dependent manner.
In Vivo

PI3K/Akt/mTOR-IN-4 (0-400 μM; 0-96 h) shows no toxicity for zebrafish embryos[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: zebrafish embryos[1]
Dosage: 0, 12.5, 25, 50, 100, 200, 400 μM
Administration: 0-96 h
Result: Showed no toxicity for zebrafish embryos.
Molecular Weight

500.56

Formula

C29H29FN4O3
SMILES

COC1=C(OC)C(OC)=CC(C2=NC3=CC(N4CCN(C)CC4)=C(C#CC5=CC=CC=C5F)C=C3N2)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

PI3K/Akt/mTOR-IN-4 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PI3K/Akt/mTOR-IN-4AktApoptosismTORPI3KMicrotubule/TubulinPKBProtein kinase BMammalian target of RapamycinPhosphoinositide 3-kinasetubulin polymerizationapoptosisG2/Mp-PI3Kp-Aktand p-mTORzebrafish embryosInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
PI3K/Akt/mTOR-IN-4
Cat. No.:
HY-163511
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.