PKC-IN-6
Based on 1 Customer Validation
PKC-IN-6 is a selective inhibitor of protein kinase C-α (PKC-α) with an IC50 of 240 μM. PKC-IN-6 shows no inhibitory activity against PKC-δ, PKA or EGF-R tyrosine kinase. PKC-IN-6 can be used in research related to bladder cancer.
For research use only. We do not sell to patients.
- Purity: 99.88%
- CAS No.: 152460-10-1
- Formula: C16H15N5
- Molecular Weight:277.33
-
Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Epigenetics Compound Library
HY-L005
-
Kinase Inhibitor Library
HY-L009
-
TGF-beta/Smad Compound Library
HY-L018
-
Anti-Cancer Compound Library
HY-L025
-
Reprogramming Compound Library
HY-L039
-
Oxygen Sensing Compound Library
HY-L045
-
Cytoskeleton Compound Library
HY-L060
-
Human Metabolite Library
HY-L123
-
Cancer Stem Cells Compound Library
HY-L135
-
Coagulation and Anti-coagulation Compound Library
HY-L136
-
Membrane Protein-targeted Compound Library
HY-L149
-
Highly Selective Inhibitors Library
HY-L158
-
Serine/Threonine Kinase Inhibitor Library
HY-L164
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Bioactive Compound Library
HY-L001
-
Anti-Brain Cancer Compound Library
HY-L188
-
Protein Kinase Compound Library
HY-L196
-
Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
-
High-Throughput Bioactive Compound Library
HY-L205
-
Mass Spectrometry Human Metabolite Library
HY-L215
-
MCE Kinase Hinge Binder Fragment Library
HY-L907
Biological Activity
|
PKC-α 240 μM (IC50) |
PKC-IN-6 (Comp 21) weakly inhibits PKC-α with an IC50 of 240 μM, and shows no inhibitory activity against PKC-δ, PKA, or EGF-R kinases[1].
PKC-IN-6 does not inhibit the growth of T24 human bladder carcinoma cells, with an IC50 > 50 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 152460-10-1
-
Appearance Solid
-
Molecular Weight 277.33
-
Formula C16H15N5
-
Color Light yellow to yellow
-
SMILES
NC1=CC(=C(C=C1)C)NC2=NC=CC(=N2)C3=CC=CN=C3
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 33.33 mg/mL (120.18 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3.33 mg/mL (12.01 mM); Clear solution
This protocol yields a clear solution of ≥ 3.33 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (33.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (270 KB)
-
SDS (454 KB)
- English - EN (454 KB)
- Français - FR (454 KB)
- Deutsch - DE (454 KB)
- Norwegian - NO (454 KB)
- Español - ES (454 KB)
- Swedish - SV (454 KB)
- Italian - IT (454 KB)
- Korean - KR (454 KB)
- Portuguese - PT (454 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.6058 mL | 18.0291 mL | 36.0581 mL | 90.1453 mL |
| 5 mM | 0.7212 mL | 3.6058 mL | 7.2116 mL | 18.0291 mL | |
| 10 mM | 0.3606 mL | 1.8029 mL | 3.6058 mL | 9.0145 mL | |
| 15 mM | 0.2404 mL | 1.2019 mL | 2.4039 mL | 6.0097 mL | |
| 20 mM | 0.1803 mL | 0.9015 mL | 1.8029 mL | 4.5073 mL | |
| 25 mM | 0.1442 mL | 0.7212 mL | 1.4423 mL | 3.6058 mL | |
| 30 mM | 0.1202 mL | 0.6010 mL | 1.2019 mL | 3.0048 mL | |
| 40 mM | 0.0901 mL | 0.4507 mL | 0.9015 mL | 2.2536 mL | |
| 50 mM | 0.0721 mL | 0.3606 mL | 0.7212 mL | 1.8029 mL | |
| 60 mM | 0.0601 mL | 0.3005 mL | 0.6010 mL | 1.5024 mL | |
| 80 mM | 0.0451 mL | 0.2254 mL | 0.4507 mL | 1.1268 mL | |
| 100 mM | 0.0361 mL | 0.1803 mL | 0.3606 mL | 0.9015 mL |
PKC-IN-6 Related Classifications
HY-W013852 Related Classifications
Powered by Bioz