Cat. No.: HY-N0439

Asiaticoside

17 Cited Publications

Customer Review

Based on 17 publication(s) in Google Scholar

Asiaticoside, a trisaccaride triterpene from Centella asiatica, suppresses TGF-β/Smad signaling through inducing Smad7 and inhibiting TGF-βRI and TGF-βRII in keloid fibroblasts; Asiaticoside shows antioxidant, anti-inflammatory, and anti-ulcer properties.

For research use only. We do not sell to patients.

Purity: 99.67%
CAS No.: 16830-15-2
Formula: C₄₈H₇₈O₁₉
Molecular Weight:959.12

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Asiaticoside Chemical Structure

CAS No.: 16830-15-2

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (17)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

TGF-beta/Smad^[1]

All p38 MAPK Isoforms :

All TGF-beta/Smad Isoforms :

Smad1
Smad3

All Endogenous Metabolite Isoforms :

In Vitro

Asiaticoside (0, 100, 250, and 500 mg/L) dose-dependently inhibits keloid fibroblasts proliferation. Asiaticoside (100, 250, and 500 mg/L) decreases the expression of collagen protein and mRNA, reduces the expression of TGF-bRI, TGF-bRII protein, and mRNA, increases the expression of Smad7, but does not affect Smad2, Smad3, Smad4, phosphorylated Smad2, and phosphorylated Smad3 in keloid fibroblasts^[1]. Asiaticoside (12.5 and 50, and 25 and 50 μg/mL) prevents endothelial cells from hypoxia-induced inhibition of cell viability and NO production. Asiaticoside (50 μg/mL) also protects endothelial cells from hypoxia-induced apoptosis and upregulates and phosphorylation of AKT/eNOS in hypoxia-exposed HPAECs^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Asiaticoside (50 mg/kg daily) blocks the development of hypoxic pulmonary hypertension (PH), cardiovascular remodeling and endothelial cell injury in rats with pulmonary hypertension^[2]. Asiaticoside (5, 15 or 45 mg/kg, p.o.) improves the learning and memory deficit, protects hippocampi against the impairment, decreases Aβ deposits in the hippocampus, and ameliorates impaired subcellular structure in rats treated with Aβ oligomers^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 16830-15-2
Appearance Solid
Molecular Weight 959.12
Formula C₄₈H₇₈O₁₉
Color White to off-white
SMILES

C[C@@H]1CC[C@@]2(C(O[C@@H]3O[C@H](CO[C@H]4[C@H](O)[C@@H](O)[C@H](O[C@@]5([H])[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O5)[C@@H](CO)O4)[C@@H](O)[C@H](O)[C@H]3O)=O)[C@@](C6=CC[C@@]([C@](C[C@@H](O)[C@H](O)[C@]7(CO)C)(C)[C@@]7([H])CC8)([H])[C@]8(C)[C@]6(C)CC2)([H])[C@H]1C
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (17)

All OthersCell Proliferation/Viability AssayCell Migration/Invasion AssayFlow CytometryWB

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Journal Impact Factor

Adv Sci (Weinh)

Targeted Extracellular Vesicles Deliver Asiaticoside to Inhibit AURKB/DRP1-Mediated Mitochondrial Fission and Attenuate Hypertrophic Scar Formation. [Abstract]

2026 Apr;13(21):e17108. PMID: 41637535
J Exp Clin Cancer Res

HOXA10 mediates epithelial-mesenchymal transition to promote gastric cancer metastasis partly via modulation of TGFB2/Smad/METTL3 signaling axis. [Abstract]

2021 Feb 9;40(1):62. PMID: 33563300
Phytomedicine

Novel AMPK/ORP8-lipophagy axis: A therapeutic target for asiaticoside-mediated cardioprotection against ischemia-reperfusion injury in hyperlipidemia. [Abstract]

2025 Sep 23:148:157317. PMID: 41027151
Biomed Pharmacother

Transforming growth factor-β receptor I kinase plays a crucial role in oligodendrocyte regeneration after demyelination. [Abstract]

2025 May 1:187:118094. PMID: 40315672
Phytother Res

Asiaticoside hampers epithelial-mesenchymal transition by promoting PPARG expression and suppressing P2RX7-mediated TGF-β/Smad signaling in triple-negative breast cancer. [Abstract]

2023 May;37(5):1771-1786. PMID: 36444395
Ecotoxicol Environ Saf

Asiaticoside ameliorates pyrimethanil-induced autophagy-dependent liver injury by suppressing CRHR1. [Abstract]

2025 Dec 2:308:119482. PMID: 41338089
Biomater Sci

Asiaticoside-laden silk nanofiber hydrogels to regulate inflammation and angiogenesis for scarless skin regeneration. [Abstract]

2021 Jul 27;9(15):5227-5236. PMID: 34190240

Asiaticoside purchased from MedChemExpress. Usage Cited in: Biomater Sci. 2021 Jul 27;9(15):5227-5236. [Abstract]

AFM images of Asiaticoside-laden silk nanofiber hydrogels with different Asiaticoside concentrations (0, 0.7, 1.4, 2.8 mg/ml). Scale bars 500 nm.
Pharmaceutics

Multifunctional PDO Thread Coated with Mg(OH)2/ZnO Nanoparticles and Asiaticoside for Improved Facial Lifting. [Abstract]

2023 Aug 28;15(9):2220. PMID: 37765189
Int Immunopharmacol

Asiaticoside promoted ferroptosis and suppressed immune escape in gastric cancer cells by downregulating the Wnt/β-catenin pathway. [Abstract]

2024 May 10:134:112175. PMID: 38733821
Int Immunopharmacol

Asiaticoside ameliorates osteoarthritis progression through activation of Nrf2/HO-1 and inhibition of the NF-κB pathway. [Abstract]

2022 Jul:108:108864. PMID: 35623293
Mol Pharm

Machine Learning Models Identify New Inhibitors for Human OATP1B1. [Abstract]

2022 Nov 7;19(11):4320-4332. PMID: 36269563
Drug Dev Res

Asiaticoside enhances the effect of propofol on the invasion, ferroptosis and immune escape of bladder cancer. [Abstract]

2024 Sep;85(6):e22242. PMID: 39161064
Microbiol Spectr

25-Hydroxycholesterol Mediates Cholesterol Metabolism to Restrict Porcine Deltacoronavirus Infection via Suppression of Transforming Growth Factor β1. [Abstract]

2022 Dec 21;10(6):e0219822. PMID: 36314946

Asiaticoside purchased from MedChemExpress. Usage Cited in: Microbiol Spectr. 2022 Dec 21;10(6):e0219822. [Abstract]

Asiaticoside (AS) (250, 500 µg/mL; 24 h) significantly inhibits the expression of mature TGF-β1 in LLC-PK1 cells.
Heliyon

Asiaticoside inhibits breast cancer progression and tumor angiogenesis via YAP1/VEGFA signal pathway. [Abstract]

2024 Aug 29;10(18):e37169. PMID: 39309801

Asiaticoside purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Aug 29;10(18):e37169. [Abstract]

Cell growth assay in MCF-7 and MDA-MB-231 cells treated with different doses of Asiaticoside (0, 0.1, 0.5, or 2 μM; 72 h).

Asiaticoside purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Aug 29;10(18):e37169. [Abstract]

Colony formation in MCF-7 and MDA-MB-231 cells treated with different doses of Asiaticoside (0, 0.1, 0.5, or 2 μM; 72 h).

Asiaticoside purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Aug 29;10(18):e37169. [Abstract]

Transwell migration assay (24 h) in MCF-7 and MDA-MB-231 cells treated with different doses of Asiaticoside (0, 0.1, 0.5, or 2 μM).

Asiaticoside purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Aug 29;10(18):e37169. [Abstract]

Flow cytometry analysis of apoptotic events in control and Asiaticoside (2 μM, 48 h)-treated cells.

Asiaticoside purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Aug 29;10(18):e37169. [Abstract]

The culture supernatants of control- and Asiaticoside (0.1, 0.5, 2 μM, 72 h)-treated breast cancer cells were collected for incubating HUVEC cells. CCK8 assay shows a decreased cell proliferation in Asiaticoside-conditioned medium-treated HUVECs.

Asiaticoside purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Aug 29;10(18):e37169. [Abstract]

The culture supernatants of control- and Asiaticoside (0.1, 0.5, 2 μM)-treated breast cancer cells were collected for incubating HUVEC cells. Transwell assay (24-h incubation) shows the impaired cell migration in Asiaticoside-conditioned medium-treated HUVECs.
J Biochem Mol Toxicol

Asiaticoside protected brain injury in hypertensive intracerebral hemorrhage via activation of the PI3K/AKT pathway. [Abstract]

2024 Sep;38(9):e23843. PMID: 39253885
Iran J Basic Med Sci

Asiaticoside attenuates hyperoxia-induced lung injury in vitro and in vivo. [Abstract]

2019 Jul;22(7):797-805. PMID: 32373302
Biochem Biophys Res Commun

The role and mechanism of asiaticoside in regulating smooth muscle cell mitochondrial Drp1 during neointimal hyperplasia and atherosclerosis. [Abstract]

2025 Dec 5:795:153102. PMID: 41371186

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (104.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.0426 mL

5.2131 mL

10.4262 mL

5 mM

0.2085 mL

1.0426 mL

2.0852 mL

10 mM

0.1043 mL

0.5213 mL

1.0426 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass

Concentration

Volume

Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (2.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (2.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (2.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage
mg/kg
Animal Weight (Per Animal)
g
Dosing Volume (Per Animal)
μL
Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Cell Assay^[1]

Cells are seeded at a density of 2-9 × 10⁴ cells/mL into 24-well plates for cell viability, 60-mm plates for RNA and protein analysis in DMEM containing 10% FBS (DMEM/10% FBS). After 24 h, medium is removed and cells are placed in serum-free DMEM. After 48 h, different concentrations of Asiaticoside in DMEM/10% FBS are added simultaneously to cells. Control cells are grown in DMEM/10% FBS without the addition of Asiaticoside. At different time points, the fibroblasts are harvested for analysis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[3]

Rats^[3] Sprague-Dawley rats are used to establish the Alzheimer's disease (AD) model. Asiaticoside is administered orally at 5, 15 or 45 mg/kg body weight per day for 7 days. The learning and memory function of the rats are evaluated by the MWM test 7 days after surgery. The swimming pool (180 cm in diameter) is maintained at 22-24°C, and divided into four quadrants with equal size. A hidden platform is placed in the center of one quadrant. Each of the cardinal points of these four quadrants is randomLy selected as the start location. On the pre-test day, rats are allowed to swim freely for 120 sec. During the test (days 1-4), each rat is subjected to 8 trials each day. The trial begins when a rat is placed in the pool, and ended when the rat finds the platform. The escape latency is recorded. If a rat fails to find the platform within 120 sec, the trial is terminated and the escape latency is recorded as 120 sec, and the rat is guided to the platform. On day 5, the swimming path is recorded by a video recording system, and the digital images are analyzed by the water maze software. In addition, the probe test is performed, in which the platform is removed, and the rat is allowed to swim freely in the pool for 120 sec. The swimming time in the target quadrant is recorded^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (287 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Tang B, et al. Asiaticoside suppresses collagen expression and TGF-β/Smad signaling through inducing Smad7 and inhibiting TGF-βRI and TGF-βRII in keloid fibroblasts. h Dermatol Res. 2011 Oct;303(8):563-72. [Content Brief]

[2]. Wang X, et al. Effect of asiaticoside on endothelial cells in hypoxia‑induced pulmonary hypertension. Mol Med Rep. 2018 Feb;17(2):2893-2900. [Content Brief]

[3]. Zhang Z, et al. Asiaticoside ameliorates β-amyloid-induced learning and memory deficits in rats by inhibiting mitochondrial apoptosis and reducing inflammatory factors. Exp Ther Med. 2017 Feb;13(2):413-420. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.0426 mL	5.2131 mL	10.4262 mL	26.0656 mL
	5 mM	0.2085 mL	1.0426 mL	2.0852 mL	5.2131 mL
	10 mM	0.1043 mL	0.5213 mL	1.0426 mL	2.6066 mL
	15 mM	0.0695 mL	0.3475 mL	0.6951 mL	1.7377 mL
	20 mM	0.0521 mL	0.2607 mL	0.5213 mL	1.3033 mL
	25 mM	0.0417 mL	0.2085 mL	0.4170 mL	1.0426 mL
	30 mM	0.0348 mL	0.1738 mL	0.3475 mL	0.8689 mL
	40 mM	0.0261 mL	0.1303 mL	0.2607 mL	0.6516 mL
	50 mM	0.0209 mL	0.1043 mL	0.2085 mL	0.5213 mL
	60 mM	0.0174 mL	0.0869 mL	0.1738 mL	0.4344 mL
	80 mM	0.0130 mL	0.0652 mL	0.1303 mL	0.3258 mL
	100 mM	0.0104 mL	0.0521 mL	0.1043 mL	0.2607 mL

Keywords

Asiaticoside

17 Cited Publications

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Asiaticoside

Apoptosis Compound Library

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Publications Citing Use of MedChemExpress (MCE) Asiaticoside

All p38 MAPK Isoforms

All TGF-beta/Smad Isoforms

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (17)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Asiaticoside Related Classifications

Cat. No.: HY-N0439