YM-1
Based on 1 publication(s) in Google Scholar
YM-1 is a stable MKT-077 (HY-15096) analog and an orally active Hsp70 inhibitor. YM-1 induces cell death of HeLa cells and up-regulates the level of p53 and p21 proteins.
For research use only. We do not sell to patients.
- Purity: 98.49%
- CAS No.: 409086-68-6
- Formula: C20H20ClN3OS2
- Molecular Weight:417.98
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Cell Cycle/DNA Damage Compound Library
HY-L004
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Immunology/Inflammation Compound Library
HY-L007
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Metabolism/Protease Compound Library
HY-L012
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Anti-Cancer Compound Library
HY-L025
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Anti-Aging Compound Library
HY-L034
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Covalent Screening Library
HY-L036
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Cytoskeleton Compound Library
HY-L060
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Orally Active Compound Library
HY-L061
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Angiogenesis-Related Compound Library
HY-L088
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Metabolic Enzyme Compound Library
HY-L146
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Membrane Protein-targeted Compound Library
HY-L149
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Extracellular Vesicles (EVs) Compound Library
HY-L168
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Multi-Target Compound Library
HY-L176
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Covalent Screening Library Plus
HY-L036P
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Bioactive Compound Library
HY-L001
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Cell Proliferation Compound Library
HY-L201
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High-Throughput Bioactive Compound Library
HY-L205
Publications Citing Use of MedChemExpress (MCE) YM-1
More
Biological Activity
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Cell Line
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Type | Value | Description | References |
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| FM3A | EC50 |
1.6 x 10-5M
Compound: 8a
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Cytotoxicity against mouse mammary tumor FM3A cells representing a model of host
Cytotoxicity against mouse mammary tumor FM3A cells representing a model of host
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[PMID: 11855978] |
| MCF7 | EC50 |
5.2 μM
Compound: YM-01
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Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTT assay
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[PMID: 24312699] |
| MDA-MB-231 | EC50 |
2 μM
Compound: YM-01
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Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTT assay
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[PMID: 24312699] |
| MEF | EC50 |
>50 μM
Compound: YM-01
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Cytotoxicity against C57BL/6 mouse MEF assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against C57BL/6 mouse MEF assessed as growth inhibition after 72 hrs by MTT assay
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[PMID: 24312699] |
YM-1 promotes Hsp70-dependent steps in nNOS maturation and partially blocks formation of the ATP-bound form[1]. YM-1 (0-200 μM) activates the binding of Hsp70 to its unfolded substrate[1]. YM-1 (0.001-1000 μM) converts Hsp70 to its tight-affinity conformation and shows binding efficacy to Hsp70 with an IC50 value of 8.2 μM[1]. YM-1 (0, 0.1, 0.5 and 1 μM; 24 hours) promotes nNOS ubiquitination[1]. YM-1 (5 and 10 μM; 24 and 48 hours) induces cell death of HeLa cells and growth arrest of hTERT-RPE1 cells[2]. YM-1 (10 μM; 48 hours) up-regulates p53 and p21 proteins and down-regulates FoxM1 and survivin[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HeLa and hTERT-RPE1 cell lines
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Concentration:10 μM
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Incubation Time:48 hoursI
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Result:Increased the level of p53 and p21 and decreased the level of FoxM1 and survivin.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:UAS-hAR52Q flies with polyQ AR-induced dihydrotestosterone (DHT) phenotype[1]
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Dosage:1 mM
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Administration:Oral administration; 1 mM, for 7 days
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Result:Weakened the DHT-dependent eye degeneration phenotype and rescued DHT-dependent pupal toxicity of the polyQ AR.
Chemical Information
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CAS No. 409086-68-6
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Appearance Solid
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Molecular Weight 417.98
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Formula C20H20ClN3OS2
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Color Brown to reddish brown
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SMILES
CN(C1=CC=CC=C1S/2)C2=C3C(N(CC)/C(S\3)=C/C4=[N+](C)C=CC=C4)=O.[Cl-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
H2O : 50 mg/mL (119.62 mM; Need ultrasonic)
DMSO : 30 mg/mL (71.77 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.98 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.98 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 2.3925 mL | 11.9623 mL | 23.9246 mL | 59.8115 mL |
| 5 mM | 0.4785 mL | 2.3925 mL | 4.7849 mL | 11.9623 mL | |
| 10 mM | 0.2392 mL | 1.1962 mL | 2.3925 mL | 5.9811 mL | |
| 15 mM | 0.1595 mL | 0.7975 mL | 1.5950 mL | 3.9874 mL | |
| 20 mM | 0.1196 mL | 0.5981 mL | 1.1962 mL | 2.9906 mL | |
| 25 mM | 0.0957 mL | 0.4785 mL | 0.9570 mL | 2.3925 mL | |
| 30 mM | 0.0797 mL | 0.3987 mL | 0.7975 mL | 1.9937 mL | |
| 40 mM | 0.0598 mL | 0.2991 mL | 0.5981 mL | 1.4953 mL | |
| 50 mM | 0.0478 mL | 0.2392 mL | 0.4785 mL | 1.1962 mL | |
| 60 mM | 0.0399 mL | 0.1994 mL | 0.3987 mL | 0.9969 mL | |
| H2O | 80 mM | 0.0299 mL | 0.1495 mL | 0.2991 mL | 0.7476 mL |
| 100 mM | 0.0239 mL | 0.1196 mL | 0.2392 mL | 0.5981 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
YM-1 Related Classifications
HY-18399 Related Classifications
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