Cat. No.: HY-W001942

2-Methylbutanoic acid

Name: 2-Methylbutanoic acid
Brand: MedChemExpress
SKU: HY-W001942
Price: 25.00 USD
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(Synonyms: 2-Methylbutyric acid)

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2-Methylbutanoic acid (2-Methylbutyric acid) is an Antifungal agent. 2-Methylbutanoic acid can be produced by Bacillus subtilis JY-7-2L. 2-Methylbutanoic acid inhibits Catalase, impairs H₂O₂ decomposition, and induces ROS production/accumulation, oxidative stress, and elevated cytosolic Ca²⁺. 2-Methylbutanoic acid inhibits primary root elongation/lateral root formation, reduces the lateral root gravitropic angle, and leaves leaf area unchanged. 2-Methylbutanoic acid inhibits Agroathelia rolfsii mycelial growth/sclerotial germination/formation, reduces the southern blight index, inhibits Geotrichum citri-aurantii, and inhibits Botrytis cinerea/Rhizopus stolonifer. 2-Methylbutanoic acid can be used in research on southern blight and citrus sour rot.

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Pureté : 99.98%
CAS No.: 116-53-0
Formule: C₅H₁₀O₂
Masse moléculaire:102.13

Stockage:

Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

2-Methylbutanoic acid Chemical Structure

CAS No.: 116-53-0

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Solvant et solubilité
- In Vitro
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target

^[1]

Microbial Metabolite

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Cellular Effect

Cell Line	Type	Value	Description	References
Hepatocyte	EC₅₀	52.14 μM Compound: 5	Reduction of propionyl-CoA in human hepatocytes derived from propionic acidemia patient pretreated for 30 mins followed by 13C-isoleucine addition and measured after 1 hr by MS/MS analysis Reduction of propionyl-CoA in human hepatocytes derived from propionic acidemia patient pretreated for 30 mins followed by 13C-isoleucine addition and measured after 1 hr by MS/MS analysis	[PMID: 33848153]

In Vitro

Fungal InfectionOxidative StressProtein/Amino Acid MetabolismOthers

Fungal Infection

2-Methylbutanoic acid (0.0-260.0 µL/L; 24-120 h; 5-day incubation) inhibits A. rolfsii mycelial growth (IC₅₀ 25.997 µL/L) and sclerotial germination in vitro, achieving 100% growth inhibition at 65.0 µL/L^[1].
2-MBA (0.0-260.0 µL/L; 7 days) inhibits the growth of A. rolfsii on A. carmichaelii root slices, achieving 100% inhibition at 65.0 µL/L^[1].
2-MBA (65.0 µL/L; 9-21 days after inoculation) reduces the white silk disease severity index of potted A. carmichaelii plants, with a maximum reduction of 44.00% at 18 days after inoculation^[1].
2-Methylbutanoic acid (0.0125-0.2 mL/L; 2-6 days) inhibits G. citri-aurantii in vitro in a concentration-dependent manner, with both MIC and MFC values of 0.1 mL/L^[5].

Oxidative Stress

2-MBA induces ROS accumulation in A. rolfsii, increases H₂O₂ content by 135%, and inhibits CAT activity by 32%^[1].
2-Methylbutanoic acid (1000 μg) triggers a rapid increase in cytosolic Ca²⁺reported by R-GECO1 and changes in oxidized glutathione reported by Grx1-roGFP2 in leaf tissues of 4-week-old Arabidopsis^[4].

Protein/Amino Acid Metabolism

2-Methylbutanoic acid (100 ppm exposure for 24 h) upregulates KAI2, MAX2, SMAX1, and SABP3 in the shoots of Arabidopsis Col-0, upregulates CML43, CPK28, and EDS1 in the roots, and upregulates TSB1 in both roots and shoots^[4].

Others

2-Methylbutanoic acid partially reproduces the Pst DC3000 mVOC-induced root architecture remodeling in Arabidopsis thaliana Col-0 seedlings, reducing primary root length, lateral root number, and LRGSA without affecting leaf area, and accounts for approximately ~70% of the primary root inhibition effect of the complete mixture^[2].
2-Methylbutanoic acid pre-exposure reduces subsequent Pst DC3000 titers by approximately 10-fold in WT and about threefold in pad3 in 4-week-old Arabidopsis^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Chemical Information

CAS No. 116-53-0
Appearance Liquid
Masse moléculaire 102.13
Formule C₅H₁₀O₂
SMILES

O=C(C(CC)C)O
Synonyms

2-Methylbutyric acid
Structure Classification
- Others
Initial Source
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvant et solubilité

In Vitro:

DMSO : 200 mg/mL (1958.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

9.7914 mL

48.9572 mL

97.9144 mL

5 mM

1.9583 mL

9.7914 mL

19.5829 mL

10 mM

0.9791 mL

4.8957 mL

9.7914 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (275 KB)
SDS (615 KB)
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COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Wang Q, et al. Antifungal potential and mechanism of 2-methylbutyric acid, a volatile organic compound produced by Bacillus subtilis JY-7-2L, against Agroathelia rolfsii. Chemical and Biological Technologies in Agriculture. 2026 Sep 30.

[2]. Kanchiswamy CN, et al. Pst DC3000 volatiles reprogram Arabidopsis root architecture through ion/redox signaling, plasmodesmal gating and PIN auxin transporters. Plant Stress. 2026 May 1;21:101438.

[3]. Flores-Tafoya PD, et al. Resin Glycosides from Ipomoea funis as Inhibitors of P‑Glycoprotein in Multidrug-Resistant Breast Carcinoma Cells. Journal of Natural Products. 2026 Jan 23;89(1):220-32.

[4]. Paponov IA, et al. Airborne Immunity: A Bacterial Volatile Primes Plant Defenses by Unlocking a Phytoalexin Biosynthetic Checkpoint. Plant, cell & environment. 2026 Nov;49(11):9070-9084. [Content Brief]

[5]. Lai W, et al. Biocontrol efficacy and mechanisms of Bacillus proteolyticus L181 against postharvest sour rot of citrus[J]. Postharvest Biology and Technology, 2026, 236: 114226.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	9.7914 mL	48.9572 mL	97.9144 mL	244.7861 mL
	5 mM	1.9583 mL	9.7914 mL	19.5829 mL	48.9572 mL
	10 mM	0.9791 mL	4.8957 mL	9.7914 mL	24.4786 mL
	15 mM	0.6528 mL	3.2638 mL	6.5276 mL	16.3191 mL
	20 mM	0.4896 mL	2.4479 mL	4.8957 mL	12.2393 mL
	25 mM	0.3917 mL	1.9583 mL	3.9166 mL	9.7914 mL
	30 mM	0.3264 mL	1.6319 mL	3.2638 mL	8.1595 mL
	40 mM	0.2448 mL	1.2239 mL	2.4479 mL	6.1197 mL
	50 mM	0.1958 mL	0.9791 mL	1.9583 mL	4.8957 mL
	60 mM	0.1632 mL	0.8160 mL	1.6319 mL	4.0798 mL
	80 mM	0.1224 mL	0.6120 mL	1.2239 mL	3.0598 mL
	100 mM	0.0979 mL	0.4896 mL	0.9791 mL	2.4479 mL

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2-Methylbutanoic acid

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