Cat. No.: HY-110398

5,6,7-Trimethoxyflavone

(Synonyms: Baicalein trimethyl ether)

1 Cited Publications

Customer Review

Based on 1 publication(s) in Google Scholar

5,6,7-Trimethoxyflavone is a flavonoid compound that can be isolated from plants Callicarpa japonica. 5,6,7-Trimethoxyflavone exhibits antiviral and anti-inflammatory activities through inhibition of NF-κB/AP-1/STAT signaling pathway.

For research use only. We do not sell to patients.

Purity: 98.02%
CAS No.: 973-67-1
Formula: C₁₈H₁₆O₅
Molecular Weight:312.32

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

5,6,7-Trimethoxyflavone Chemical Structure

CAS No.: 973-67-1

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

p38-α MAPK^[1]

All NF-κB Isoforms :

All AP-1 Isoforms :

All STAT Isoforms :

All HSV Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	GI₅₀	53.99 μM Compound: 2	Growth inhibition of human HepG2 cells by MTT assay Growth inhibition of human HepG2 cells by MTT assay	[PMID: 27503682]
KB	IC₅₀	85.9 μM Compound: 6	Inhibitory activity against KB cell line after 72 h of drug exposure Inhibitory activity against KB cell line after 72 h of drug exposure	[PMID: 15481991]
KB 3-1	EC₅₀	5.5 μM Compound: 6	Concentration that causes 50% of maximum vinblastine accumulation in KB/MDR cells in 1 h Concentration that causes 50% of maximum vinblastine accumulation in KB/MDR cells in 1 h	[PMID: 15481991]
KB 3-1	IC₅₀	57.9 μM Compound: 6	Inhibitory activity against KB/MDR cell line after 72 hr of drug exposure Inhibitory activity against KB/MDR cell line after 72 hr of drug exposure	[PMID: 15481991]
MCF7	GI₅₀	91.4 μM Compound: 2	Growth inhibition of human MCF7 cells by MTT assay Growth inhibition of human MCF7 cells by MTT assay	[PMID: 27503682]
RAW264.7	IC₅₀	>100 μM Compound: 3a	Cytotoxicity against mouse RAW264.7 cells Cytotoxicity against mouse RAW264.7 cells	[PMID: 22101131]

In Vitro

5,6,7-Trimethoxyflavone exhibits inhibitory effects on herpes simplex virus type 1 (HSV-1), human cytomegalovirus (hCMV), and poliovirus, inhibits strains HSV-1 HF, HSV-1 KOS, HSV-1 A4-3, HCMV Towne and Poliovirus P1 Sabin with IC₅₀s of 3.2 mg/L, 5.8 mg/L, 9.4 mg/L, 8 mg/L and 32 mg/L, respectively^[1].
5,6,7-Trimethoxyflavone exhibits slight cytotoxicity, CC₅₀s for Vero and MRC-5 are 263.13 mg/L and 37.42 mg/L^[1].
5,6,7-Trimethoxyflavone (10-40 µM, 24 h) inhibits the production of NO, PGE2, iNOS and COX-2, reduces the mRNA expression of TNF-α, IL-1β and IL-6 in LPS (HY-D1056)-stimulated RAW 264.7 cells^[2].
5,6,7-Trimethoxyflavone inhibits the transcriptional activity and nuclear translocation of NF-κB, AP-1 and STAT1/3, inhibits the phosphorylation and degradation of IκBα, and inhibits the phosphorylation of p38 MAPK^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[2]

Cell Line:

RAW 264.7
Concentration:

10-40 µM
Incubation Time:

24 h
Result:

Inhibited the expressions of iNOS, COX-2, TNF-α, IL-1β and IL-6.

In Vivo

5,6,7-Trimethoxyflavone (10-40 mg/kg, ip, single dose) exhibits anti-inflammatory activity, prolongs the survival time of LPS (HY-D1056)-induced sepsis mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Mouse LPS (HY-D1056)-induced sepsis models^[2]
Dosage:

10-40 mg/kg
Administration:

ip, single dose
Result:

Prolonged the survival time of mice.
Reduced serum TNF-α, IL-1β and IL-6 levels.

Chemical Information

CAS No. 973-67-1
Appearance Solid
Molecular Weight 312.32
Formula C₁₈H₁₆O₅
Color White to light yellow
SMILES

O=C1C=C(C2=CC=CC=C2)OC3=CC(OC)=C(OC)C(OC)=C13
Synonyms

Baicalein trimethyl ether
Structure Classification
- Flavonoids
- Flavones
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

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Journal Impact Factor

Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (106.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.2018 mL

16.0092 mL

32.0184 mL

5 mM

0.6404 mL

3.2018 mL

6.4037 mL

10 mM

0.3202 mL

1.6009 mL

3.2018 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (8.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (8.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Hayashi K, et al., Antiviral activity of 5,6,7-trimethoxyflavone and its potentiation of the antiherpes activity of acyclovir. J Antimicrob Chemother. 1997 Jun;39(6):821-4. [Content Brief]

[2]. Rim HK, et al., 5,6,7-trimethoxyflavone suppresses pro-inflammatory mediators in lipopolysaccharide-induced RAW 264.7 macrophages and protects mice from lethal endotoxin shock. Food Chem Toxicol. 2013 Dec;62:847-55. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.2018 mL	16.0092 mL	32.0184 mL	80.0461 mL
	5 mM	0.6404 mL	3.2018 mL	6.4037 mL	16.0092 mL
	10 mM	0.3202 mL	1.6009 mL	3.2018 mL	8.0046 mL
	15 mM	0.2135 mL	1.0673 mL	2.1346 mL	5.3364 mL
	20 mM	0.1601 mL	0.8005 mL	1.6009 mL	4.0023 mL
	25 mM	0.1281 mL	0.6404 mL	1.2807 mL	3.2018 mL
	30 mM	0.1067 mL	0.5336 mL	1.0673 mL	2.6682 mL
	40 mM	0.0800 mL	0.4002 mL	0.8005 mL	2.0012 mL
	50 mM	0.0640 mL	0.3202 mL	0.6404 mL	1.6009 mL
	60 mM	0.0534 mL	0.2668 mL	0.5336 mL	1.3341 mL
	80 mM	0.0400 mL	0.2001 mL	0.4002 mL	1.0006 mL
	100 mM	0.0320 mL	0.1601 mL	0.3202 mL	0.8005 mL

Keywords

5,6,7-Trimethoxyflavone

1 Cited Publications

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5,6,7-Trimethoxyflavone

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Publications Citing Use of MedChemExpress (MCE) 5,6,7-Trimethoxyflavone

All NF-κB Isoforms

All AP-1 Isoforms

All STAT Isoforms

All HSV Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

5,6,7-Trimethoxyflavone Related Classifications

HY-110398 Related Classifications