Danazol

Name: Danazol
Brand: MedChemExpress
SKU: HY-B1029
Rating: 5.0 (4 reviews)

Cat. No.: HY-B1029 Purity: 99.66%: Data Sheet
COA

SDS
Handling Instructions
FAQs
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Danazol inhibits the proliferation of cancer cell MDA-MB-231 and MCF-7 with IC₅₀ of 65 µg/mL and 31 µg/mL. Danazol arrests the cell cycle at G1 phase, induces apoptosis in MDA-MB-231 through PKCα signaling pathway.

For research use only. We do not sell to patients.

CAS No. : 17230-88-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
250 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	Get quote
1 g	Get quote

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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Danazol:

Danazol (Standard) Get quote

4 Publications Citing Use of MCE Danazol

In Vivo Efficacy Study

: Danazol purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Apr 16;10(9):e29723. [Abstract]; The rats were injected with Danazol on PND5 to induce the ICPP rat model of ICPP. The ICPP rats in the positive control group were further treated with ZBDH and GnRHa, respectively.

: Danazol purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Apr 16;10(9):e29723. [Abstract]; As shown by the results, the vaginal opening of Danazol-induced ICPP rats occurred on the PND17, which was significantly earlier than that of normal control rats; however, ZBDH and GnRHa treatments could effectively delay the vaginal opening time of ICPP rats.

: Danazol purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Apr 16;10(9):e29723. [Abstract]; The Danazol-induced idiopathic central precocious puberty (ICPP) rat model was successfully constructed and ZBDH increased the vaginal opening time. The normal estrus cycle was displayed.

: Danazol purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Apr 16;10(9):e29723. [Abstract]; The results revealed that ZBDH and GnRHa could inhibit the increase of serum hormones in Danazol-induced ICPP rats.

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PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKC-ι ℩ PKCι

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Danazol (0-100 μM, 18-72 h) inhibits the proliferation and the formation of capillary-like tubular structures in HUVECs^[2].
Danazol (5-20 μM, 48 h) inhibits melanogenesis in B16 cell with an IC₅₀ of 9.3 μM by post-transcriptionally inhibiting the expression of tyrosinase protein^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:	HUVEC
Concentration:	0-100 μM
Incubation Time:	24-72 h
Result:	Inhibited the proliferation of HUVECs.

Western Blot Analysis^[2]

Cell Line:	B16
Concentration:	5-20 μM
Incubation Time:	48 h
Result:	Inhibited the expression of tyrosinase protein.

Clinical Trial

Molecular Weight

337.46

Formula

C₂₂H₂₇NO₂

CAS No.

17230-88-5

Appearance

Solid

Color

White to off-white

SMILES

C#C[C@]1(O)CC[C@@]2([H])[C@]3([H])CCC4=CC5=C(C=NO5)C[C@]4(C)[C@@]3([H])CC[C@@]21C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (74.08 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 20 mg/mL (59.27 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9633 mL	14.8166 mL	29.6331 mL
5 mM	0.5927 mL	2.9633 mL	5.9266 mL
10 mM	0.2963 mL	1.4817 mL	2.9633 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (7.41 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 3

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2 mg/mL (5.93 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 4

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2 mg/mL (5.93 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.66%

Select Batch:

Data Sheet (279 KB) SDS (557 KB)

COA (253 KB) LCMS (267 KB)

Handling Instructions (2659 KB)

References

[1]. Thomas GW, et al., Effects of danazol on endothelial cell function and angiogenesis. Fertil Steril. 2007 Oct;88(4 Suppl):1065-70. [Content Brief]

[2]. Chang TS, Lin JJ. Inhibitory effect of danazol on melanogenesis in mouse B16 melanoma cells. Arch Pharm Res. 2010 Dec;33(12):1959-65. [Content Brief]

[3]. Deka SJ, et al., Danazol has potential to cause PKC translocation, cell cycle dysregulation, and apoptosis in breast cancer cells. Chem Biol Drug Des. 2017 Jun;89(6):953-963. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	2.9633 mL	14.8166 mL	29.6331 mL	74.0829 mL
	5 mM	0.5927 mL	2.9633 mL	5.9266 mL	14.8166 mL
	10 mM	0.2963 mL	1.4817 mL	2.9633 mL	7.4083 mL
	15 mM	0.1976 mL	0.9878 mL	1.9755 mL	4.9389 mL
	20 mM	0.1482 mL	0.7408 mL	1.4817 mL	3.7041 mL
	25 mM	0.1185 mL	0.5927 mL	1.1853 mL	2.9633 mL
	30 mM	0.0988 mL	0.4939 mL	0.9878 mL	2.4694 mL
	40 mM	0.0741 mL	0.3704 mL	0.7408 mL	1.8521 mL
	50 mM	0.0593 mL	0.2963 mL	0.5927 mL	1.4817 mL
DMSO	60 mM	0.0494 mL	0.2469 mL	0.4939 mL	1.2347 mL

Danazol Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Danazol17230-88-5PKCApoptosisTyrosinaseProtein kinase CInhibitorinhibitorinhibit

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Danazol

Customer Review

Other Forms of Danazol:

Danazol Related Antibodies

4 Publications Citing Use of MCE Danazol

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All PKC Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Danazol Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Danazol Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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