2. Anti-infection Apoptosis Autophagy
  3. Bacterial Antibiotic Apoptosis Autophagy
  4. Nitroxoline

Nitroxoline  (Synonyms: 8-Hydroxy-5-nitroquinoline; 5-Nitro-8-quinolinol)

Cat. No.: HY-B1159 Purity: 99.57%
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Nitroxoline (8-Hydroxy-5-nitroquinoline), an antibiotic, is an orally active antibiofilm agent. Nitroxoline reduces the formation and induces the dispersal of Pseudomonas aeruginosa biofilms by chelation of iron and zinc. Nitroxoline can be used for the urinary tract infections and cancer research.

For research use only. We do not sell to patients.

Nitroxoline Chemical Structure

Nitroxoline Chemical Structure

CAS No. : 4008-48-4

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Based on 1 publication(s) in Google Scholar

Other Forms of Nitroxoline:

Nitroxoline Related Antibodies

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Description

Nitroxoline (8-Hydroxy-5-nitroquinoline), an antibiotic, is an orally active antibiofilm agent. Nitroxoline reduces the formation and induces the dispersal of Pseudomonas aeruginosa biofilms by chelation of iron and zinc. Nitroxoline can be used for the urinary tract infections and cancer research[1][2][3].

In Vitro

Biofilm mass synthesis is reduced by up to 80% at sub-MIC Nitroxoline (0.25-16 μg/mL) concentrations in Pseudomonas aeruginosa, and structures formed are reticulate rather than compact. In preformed biofilms, viable cell counts are reduced by 4 logs at therapeutic concentrations[1].
Nitroxoline (2.5-20 μM; 24 hours) effectively inhibits cell survival of small-cell lung cancer (SCLC) cells, and induces SCLC cell apoptosis by suppressing antiapoptotic proteins (such as Bcl-2 and MCL1) and upregulating proapoptotic protein Bim. Nitroxoline is found to downregulate MDM2 expression by inducing its proteasomal degradation, and thus upregulates p53 expression, which is a substrate protein of MDM2[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Nitroxoline Related Antibodies

Cell Viability Assay[2]

Cell Line: H446, H1882, H1417, and H1688 cells
Concentration: 2.5 μM, 5 μM, 10 μM, 20 μM
Incubation Time: 24 hours
Result: Effectively inhibit cell survival of small-cell lung cancer (SCLC) cells.

Western Blot Analysis[2]

Cell Line: H446, H1882, H1417 cells
Concentration: 5 μM, 10 μM, 20 μM
Incubation Time: 24 hours
Result: Suppressed antiapoptotic proteins (such as Bcl-2 and MCL1) and upregulated proapoptotic protein Bim.
In Vivo

Nitroxoline (15-60mg/kg; orally gavage) results in significant inhibition of tumor growth in the C3H/He mice bladder cancer subcutaneous model[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C3H/He mice bladder injected with MBT-2 cells[3]
Dosage: 15 mg/kg or 60mg/kg
Administration: Orally gavage; five times a week on days 1, 2, 4, 5, 7, 8, 10, and 11.
Result: Resulted in significant inhibition of tumor growth.
Molecular Weight

190.16

Formula

C9H6N2O3
CAS No.
Appearance

Solid

Color

Light yellow to green yellow

SMILES

OC1=C2N=CC=CC2=C([N+]([O-])=O)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (262.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.2587 mL 26.2936 mL 52.5873 mL
5 mM 1.0517 mL 5.2587 mL 10.5175 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.57%

COA (253 KB) HNMR (241 KB) LCMS (270 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.2587 mL 26.2936 mL 52.5873 mL 131.4682 mL
5 mM 1.0517 mL 5.2587 mL 10.5175 mL 26.2936 mL
10 mM 0.5259 mL 2.6294 mL 5.2587 mL 13.1468 mL
15 mM 0.3506 mL 1.7529 mL 3.5058 mL 8.7645 mL
20 mM 0.2629 mL 1.3147 mL 2.6294 mL 6.5734 mL
25 mM 0.2103 mL 1.0517 mL 2.1035 mL 5.2587 mL
30 mM 0.1753 mL 0.8765 mL 1.7529 mL 4.3823 mL
40 mM 0.1315 mL 0.6573 mL 1.3147 mL 3.2867 mL
50 mM 0.1052 mL 0.5259 mL 1.0517 mL 2.6294 mL
60 mM 0.0876 mL 0.4382 mL 0.8765 mL 2.1911 mL
80 mM 0.0657 mL 0.3287 mL 0.6573 mL 1.6434 mL
100 mM 0.0526 mL 0.2629 mL 0.5259 mL 1.3147 mL
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Nitroxoline Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nitroxoline4008-48-48-Hydroxy-5-nitroquinoline 5-Nitro-8-quinolinolBacterialAntibioticApoptosisAutophagyInhibitorinhibitorinhibit

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