1. Metabolic Enzyme/Protease
  2. MMP
  3. Ro 31-9790

Ro 31-9790 (Synonyms: GI4747)

Cat. No.: HY-101703
Handling Instructions

Ro 31-9790 is a synthetic metalloproteinase (MMP) inhibitor.

For research use only. We do not sell to patients.

Ro 31-9790 Chemical Structure

Ro 31-9790 Chemical Structure

CAS No. : 145337-55-9

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Description

Ro 31-9790 is a synthetic metalloproteinase (MMP) inhibitor.

IC50 & Target

MMP[1]

In Vitro

Ro 31-9790 inhibits L-selectin shedding from mouse and human lymphocytes, Jurkat T cells, and human monocytes with an IC50 of 0.3-0.4 μM on these cell types. The IC50 values obtained for Ro 31-9790 are 4.82 ± 0.75 μM, 1.16 ± 0.27 μM, 0.70 ± 0.06 μM, 4.47± 1.27 μM and 0.38 ± 0.05 μM for mouse lymphocyte L-selectin shedding, Jurkat L-selectin shedding, human lymphocyte L-selectin shedding, human monocyte L-selectin shedding and human monocyte TNF-α shedding[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

315.41

Formula

C₁₅H₂₉N₃O₄

CAS No.

145337-55-9

SMILES

O=C(N[[email protected]](C(NC)=O)C(C)(C)C)[[email protected]](CC(C)C)CC(NO)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
Cell Assay
[1]

For mouse and human lymphocytes and Jurkat T cells, 100% inhibition is set as the percentage of cells positive for L-selectin in an untreated sample (after flow cytometric analysis) or the concentration of soluble L-selectin in the supernatant of an untreated cell sample (after ELISA); 0% inhibition is set at the appropriate value for a PMA-treated sample. For results from human monocytes, a different calculation is needed to allow direct comparison of L-selectin and TNF-α shedding. Therefore, in this case 100% inhibition is set as the percentage of cells positive for L-selectin in the presence of PMA+50 μM Ro 31-9790 or the percentage of cells positive for cell surface TNF-α in the presence of LPS+50 μM Ro 31-9790 (doses of Ro 31-9790 which gave maximal inhibition); 0% inhibition is set as the percentage of cells positive for L-selectin or TNF-α in the presence of PMA or LPS, respectively. Intermediate percent inhibition is calculated. The IC50 value for inhibitor in each system is defined as the concentration of inhibitor which gave 50% inhibition, where 100% inhibition is set as the percentage of cells positive for L-selectin or cell surface TNF-α in the presence of Phorbol myristate acetate (PMA)+50 μM Ro 31-9790 or Lipopolysaccharide (LPS)+50 μM Ro 31-9790, respectively (doses of Ro 31-9790 which gave maximal inhibition) and 0% inhibition is set as the percentage of cells positive for L-selectin or TNF-α in the presence of PMA or LPS alone, respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Keywords:

Ro 31-9790GI4747GI 4747GI-4747MMPMatrix metalloproteinasesInhibitorinhibitorinhibit

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Ro 31-9790
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HY-101703
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