VER-155008
Based on 44 publication(s) in Google Scholar
VER-155008 is an inhibitor of Hsp70, with IC50s of 0.5 μM, 2.6 μM, and 2.6 μM for Hsp70, Hsc70 and Grp7, respectively, and with a Kd of 0.3 μM for Hsp70.
For research use only. We do not sell to patients.
- Purity: 99.82%
- CAS No.: 1134156-31-2
- Formula: C25H23Cl2N7O4
- Molecular Weight:556.40
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Protocol
- Purity & Documentation
- References
-
Help & FAQs
-
Cell Cycle/DNA Damage Compound Library
HY-L004
-
Metabolism/Protease Compound Library
HY-L012
-
Anti-Cancer Compound Library
HY-L025
-
CNS-Penetrant Compound Library
HY-L028
-
Autophagy Compound Library
HY-L029
-
Anti-Aging Compound Library
HY-L034
-
Nucleotide Compound Library
HY-L044
-
Cytoskeleton Compound Library
HY-L060
-
Targeted Diversity Library
HY-L099
-
Cuproptosis Compound Library
HY-L133
-
Metabolic Enzyme Compound Library
HY-L146
-
Highly Selective Inhibitors Library
HY-L158
-
Cell Death Library
HY-L162
-
Extracellular Vesicles (EVs) Compound Library
HY-L168
-
Multi-Target Compound Library
HY-L176
-
Bioactive Compound Library Max
HY-L181
-
Anti-Aging Compound Library Mini
HY-L034M
-
MCE Bioactive Compound Library
HY-L001V
-
Bioactive Compound Library
HY-L001
-
Anti-Brain Cancer Compound Library
HY-L188
-
High-Throughput Bioactive Compound Library
HY-L205
Publications Citing Use of MedChemExpress (MCE) VER-155008
More- Nat Biotechnol. 2025 Sep 10. [Abstract]
- Adv Funct Mater. 2025 Jun 20.
- Mol Cell. 2026 Feb 19;86(4):674-692.e10. [Abstract]
- Mol Cell. 2026 Jan 14;86(2):345-361.e6.
- Nat Commun. 2025 Apr 22;16(1):3777. [Abstract]
- Adv Sci (Weinh). 2025 Jul 2:e06639. [Abstract]
- Sci Adv. 2022 Jun 10;8(23):eabm7981. [Abstract]
- Redox Biol. 2024 Apr:70:103035. [Abstract]
- Cell Rep Phys Sci. 2025 Jun 18;6(6).
- Cell Rep. 2025 Feb 19;44(3):115339. [Abstract]
- J Med Chem. 2025 Jan 23;68(2):1619-1631. [Abstract]
- Phytother Res. 2018 Jul;32(7):1320-1331. [Abstract]
- EMBO Rep. 2025 Mar;26(5):1344-1366. [Abstract]
- Food Biosci. 2025 Nov 17;74:107954.
- Int J Mol Sci. 2025 Oct 13;26(20):9963. [Abstract]
- Int J Mol Sci. 2025 Mar 20;26(6):2804. [Abstract]
- Mol Plant Pathol. 2022 Jun;23(6):805-818. [Abstract]
- Food Microbiol. 2026 Jan:133:104879. [Abstract]
- Molecules. 2023 Nov 17;28(22):7639. [Abstract]
- Mol Oncol. 2026 Jun 5. [Abstract]
- Comp Biochem Physiol C Toxicol Pharmacol. 2025 Jul:293:110164. [Abstract]
- Exp Neurol. 2026 May:399:115673. [Abstract]
- Poult Sci. 2025 Dec 24;105(2):106336. [Abstract]
- J Biol Chem. 2023 Jun;299(6):104814. [Abstract]
- J Biol Chem. 2023 May;299(5):104668. [Abstract]
- J Virol. 2025 Dec 23;99(12):e0108925. [Abstract]
- J Virol. 2025 Jun 5:e0050225. [Abstract]
- Microbiol Spectr. 2022 Feb 23;10(1):e0186021. [Abstract]
- Biochim Biophys Acta Mol Cell Res. 2025 Jul 24;1872(8):120027. [Abstract]
- J Cell Sci. 2025 Dec 19:jcs.264115. [Abstract]
- Vet Res. 2024 Dec 18;55(1):158. [Abstract]
- Microb Pathog. 2021 Apr;153:104647. [Abstract]
- Front Physiol. 2021 Feb 19;12:609674. [Abstract]
- Pathogens. 2021 Mar 2;10(3):283. [Abstract]
- Am J Physiol Gastrointest Liver Physiol. 2020 Jun 1;318(6):G1000-G1012. [Abstract]
- Mol Biol Cell. 2024 Jul 3:mbcE24040194. [Abstract]
- Biochem Biophys Res Commun. 2025 Sep 16:780:152466. [Abstract]
- Domest Anim Endocrin. 2021 Jan;74:106533. [Abstract]
- bioRxiv. 2025 Sep 2.
- bioRxiv. 2025 Jul 12:2025.07.08.663754. [Abstract]
- bioRxiv. 2025 Mar 26:2025.02.20.639340. [Abstract]
- bioRxiv. 2024 October 06.
- bioRxiv. 2024 August 18.
- bioRxiv. 2020 Jul.
Customer Validation & Images
-
Cell Proliferation/Viability Assay
-
Flow Cytometry
-
WB
-
WB
-
Cell Proliferation/Viability Assay
Biological Activity
|
HSP70 0.5 μM (IC50) |
HSC70 2.6 μM (IC50) |
Grp78 2.6 μM (IC50) |
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| BaF3 | EC50 |
5.5 μM
Compound: VER-155008
|
Induction of apoptosis in mouse BaF3 cells incubated for 24 hrs by annexin V/PI staining based flow cytometry analysis
Induction of apoptosis in mouse BaF3 cells incubated for 24 hrs by annexin V/PI staining based flow cytometry analysis
|
[PMID: 38011535] |
| BaF3 | EC50 |
7.73 μM
Compound: VER-155008
|
Induction of apoptosis in mouse BaF3 cells incubated for 48 hrs by Annexin V-FITC staining based flow cytometry analysis
Induction of apoptosis in mouse BaF3 cells incubated for 48 hrs by Annexin V-FITC staining based flow cytometry analysis
|
[PMID: 39012838] |
| BT-474 | IC50 |
10.4 μM
Compound: 5, VER-155008
|
Binding affinity to Hsp70 nucleotide binding domain in human BT474 cells
Binding affinity to Hsp70 nucleotide binding domain in human BT474 cells
|
[PMID: 20608738] |
| BV-173 | EC50 |
5.64 μM
Compound: VER-155008
|
Induction of apoptosis in human BV-173 cells incubated for 48 hrs by Annexin V-FITC staining based flow cytometry analysis
Induction of apoptosis in human BV-173 cells incubated for 48 hrs by Annexin V-FITC staining based flow cytometry analysis
|
[PMID: 39012838] |
| BV-173 | EC50 |
5.8 μM
Compound: VER-155008
|
Induction of apoptosis in human BV-173 cells incubated for 24 hrs by annexin V/PI staining based flow cytometry analysis
Induction of apoptosis in human BV-173 cells incubated for 24 hrs by annexin V/PI staining based flow cytometry analysis
|
[PMID: 38011535] |
| HCT-116 | GI50 |
5 μM
Compound: 12
|
Cytotoxicity against human HCT116 cells by SRB assay
Cytotoxicity against human HCT116 cells by SRB assay
|
[PMID: 19256508] |
| HCT-116 | GI50 |
5 μM
Compound: 13, VER-155008
|
Antiproliferative activity against human HCT116 cells
Antiproliferative activity against human HCT116 cells
|
[PMID: 21526763] |
| HEK293 | EC50 |
6.46 μM
Compound: VER-155008
|
Induction of apoptosis in HEK293 cells incubated for 48 hrs by Annexin V-FITC staining based flow cytometry analysis
Induction of apoptosis in HEK293 cells incubated for 48 hrs by Annexin V-FITC staining based flow cytometry analysis
|
[PMID: 39012838] |
| HEK293 | EC50 |
7.7 μM
Compound: VER-155008
|
Induction of apoptosis in human HEK293 cells incubated for 24 hrs by annexin V/PI staining based flow cytometry analysis
Induction of apoptosis in human HEK293 cells incubated for 24 hrs by annexin V/PI staining based flow cytometry analysis
|
[PMID: 38011535] |
| K562 | EC50 |
7.05 μM
Compound: VER-155008
|
Induction of apoptosis in human K562 cells incubated for 48 hrs by Annexin V-FITC staining based flow cytometry analysis
Induction of apoptosis in human K562 cells incubated for 48 hrs by Annexin V-FITC staining based flow cytometry analysis
|
[PMID: 39012838] |
| K562 | EC50 |
7.8 μM
Compound: VER-155008
|
Induction of apoptosis in human K562 cells incubated for 24 hrs by annexin V/PI staining based flow cytometry analysis
Induction of apoptosis in human K562 cells incubated for 24 hrs by annexin V/PI staining based flow cytometry analysis
|
[PMID: 38011535] |
VER-155008 is an inhibitor of Hsc70 and Hsp70, with IC50s of 0.5 μM, 2.6 μM, and 2.6 μM for Hsp70, Hsc70 and Grp7, respectively, a with a Kd of 0.3 μM for Hsp70, but shows no activities against Hsp90, with an IC50 of >200 μM. VER-155008 inhibits the proliferation of a variety of human colon and breast tumor cell lines, such as BT474, MB-468, HCT116 and HT29 cells, with GI50s of 10.4 μM, 14.4 μM, 5.3 μM, and 12.8 μM, respectively. VER-155008 (5-40 μM) induces client protein degradation in HCT116 and BT474 carcinoma cells. VER-155008 also induces apoptosis in human tumor cell lines[1]. VER-155008 (0.05-5 μM) reverses Aβ-induced axonal degeneration in cultured neurons[2]. VER-155008 (10 μM or 25 μM) inhibits Hsp70 and suppresses the proliferation of LNCaP95 cells. VER-155008 also reduces full-length androgen receptor (AR-FL) and androgen receptor splice variant 7 (AR-V7) protein expression[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 1134156-31-2
-
Appearance Solid
-
Molecular Weight 556.40
-
Formula C25H23Cl2N7O4
-
Color White to pink
-
SMILES
NC1=C2N=C(NCC3=CC=C(Cl)C(Cl)=C3)N([C@@H]([C@H](O)[C@@H]4O)O[C@@H]4COCC5=CC=C(C#N)C=C5)C2=NC=N1
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (44)
-
Journal Impact Factor
-
Most Recent
-
Nat Biotechnol
A rapid imaging-based screen for induced-proximity degraders identifies a potent degrader of oncoprotein SKP2. [Abstract]2025 Sep 10. PMID: 40931108 -
-
Mol Cell
HSPA1A and DNAJB1 regulate NELF condensate dynamics to safeguard transcriptional recovery under heat stress. [Abstract]2026 Feb 19;86(4):674-692.e10. PMID: 41653920 -
-
Nat Commun
Chloroplast precursor protein preClpD overaccumulation triggers multilevel reprogramming of gene expression and a heat shock-like response. [Abstract]2025 Apr 22;16(1):3777. PMID: 40263324 -
Adv Sci (Weinh)
Intermediate Filament Protein BFSP2 Controls Spindle Formation via HSC70-Mediated Stabilization of CLTC During Oocyte meiosis. [Abstract]2025 Jul 2:e06639. PMID: 40600490 -
Sci Adv
Single-cell analysis of human basal cell carcinoma reveals novel regulators of tumor growth and the tumor microenvironment. [Abstract]2022 Jun 10;8(23):eabm7981. PMID: 35687691
VER-155008 purchased from MedChemExpress. Usage Cited in: Sci Adv. 2022 Jun 10;8(23):eabm7981. [Abstract]
Western blot of RFI (Relative Fluorescence Intensity) against Hsp70 in ASZ001 murine cells treated with HSP inhibitor Ver155008 (25 μM, 24 h).
VER-155008 purchased from MedChemExpress. Usage Cited in: Sci Adv. 2022 Jun 10;8(23):eabm7981. [Abstract]
HSP inhibitor Ver155008 (25 μM, 24 h) negatively affects growth of ASZ001 murine cells.
VER-155008 purchased from MedChemExpress. Usage Cited in: Sci Adv. 2022 Jun 10;8(23):eabm7981. [Abstract]
H&E of Ver155008 (16 mg/kg intraperitoneally for seven consecutive days)- and vehicle-treated Gli1-CreERT2;Ptch1fl/fl mouse dorsal skin tissues.Quantification of microtumor surface area in vehicle- and Ver155008-treated Gli1-CreERT2;Ptch1fl/fl mouse dorsal skin tissues. Bars represent average individual microtumor area ± SEM. Surface area decreased in a concentration-dependent manner compared to vehicle-treated control mice. Unpaired Student’s two-tailed t test (*P < 0.05).
-
Redox Biol
DNAJC12 causes breast cancer chemotherapy resistance by repressing doxorubicin-induced ferroptosis and apoptosis via activation of AKT. [Abstract]2024 Apr:70:103035. PMID: 38306757
VER-155008 purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Apr:70:103035. [Abstract]
Cell viability measured by CCK-8 after cells were treated with or without 2 μM VER-155008 (48 h) in MCF-7.
VER-155008 purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Apr:70:103035. [Abstract]
Flow cytometry assay results of cleaved caspase 3 were used to analyze apoptosis in cells treated with or without 2 μM VER-155008 (48 h) in MDA-MB-231 and in MCF-7.
VER-155008 purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Apr:70:103035. [Abstract]
Western blot results of cleaved caspase 3 were used to analyze apoptosis in cells treated with or without 2 μM VER-155008 (48 h) in MDA-MB-231 and in MCF-7.
-
-
Cell Rep
HSPA8 dampens SCAP/INSIG split and SREBP activation by reducing PKR-mediated INSIG phosphorylation. [Abstract]2025 Feb 19;44(3):115339. PMID: 39977267 -
J Med Chem
Discovery of D-Ring-Contracted Artemisinin as a Potent Hydrophobic Tag for Targeted Protein Degradation. [Abstract]2025 Jan 23;68(2):1619-1631. PMID: 39748630 -
Phytother Res
2018 Jul;32(7):1320-1331. PMID: 29516568
VER-155008 purchased from MedChemExpress. Usage Cited in: Phytother Res. 2018 Jul;32(7):1320-1331. [Abstract]
Panc-28 cells are treated with VER-155008 (20 μM) for 48 hr, and the expression of HSPA8 is determined using western blot
-
EMBO Rep
HSC70 coordinates COP9 signalosome and SCF ubiquitin ligase activity to enable a prompt stress response. [Abstract]2025 Mar;26(5):1344-1366. PMID: 39915298 -
-
Int J Mol Sci
HSP90 Inhibition Disrupts 27-Hydroxycholesterol-Induced Inflammatory Signaling in Monocytic Cells. [Abstract]2025 Oct 13;26(20):9963. PMID: 41155259 -
Int J Mol Sci
TBG096 Ameliorates Memory Deficiency in AD Mouse Model via Promoting Neurogenesis and Regulation of Hsc70/HK2/PKM2/LAMP2A Signaling Pathway. [Abstract]2025 Mar 20;26(6):2804. PMID: 40141445 -
Mol Plant Pathol
A plant reovirus hijacks the DNAJB12-Hsc70 chaperone complex to promote viral spread in its planthopper vector. [Abstract]2022 Jun;23(6):805-818. PMID: 34668642 -
Food Microbiol
Dual-phase Hog1 activation and transporter gene reprogramming enable extreme sugar tolerance in food osmophilic yeasts. [Abstract]2026 Jan:133:104879. PMID: 40889845 -
Molecules
Vitexin Regulates Heat Shock Protein Expression by Modulating ROS Levels Thereby Protecting against Heat-Stress-Induced Apoptosis. [Abstract]2023 Nov 17;28(22):7639. PMID: 38005362 -
Mol Oncol
Patient therapy outcome modeling in cancer organoids is improved by cancer-associated fibroblasts and organoid assembly convolution. [Abstract]2026 Jun 5. PMID: 42246237 -
Comp Biochem Physiol C Toxicol Pharmacol
Bisphenol AF induces lipid metabolism disorders, oxidative stress and upregulation of heat shock protein 70 in zebrafish. [Abstract]2025 Jul:293:110164. PMID: 40020955 -
Exp Neurol
The crosstalk between chaperone-mediated autophagy and apoptosis via ATM/p53-mitochondria-dependent signaling contributed to ischemia-induced cerebral injuries. [Abstract]2026 May:399:115673. PMID: 41620198 -
Poult Sci
Targeting the pan-viral host protein HSPA8 with oxymatrine as a novel therapy against duck hepatitis a virus infection. [Abstract]2025 Dec 24;105(2):106336. PMID: 41455214 -
J Biol Chem
Diarylheptanoid 35d overcomes EGFR TKI resistance by inducing hsp70-mediated lysosomal degradation of EGFR in EGFR-mutant lung adenocarcinoma. [Abstract]2023 Jun;299(6):104814. PMID: 37178919 -
J Biol Chem
Heat shock protein 90 facilitates SARS-CoV-2 structural protein-mediated virion assembly and promotes virus-induced pyroptosis. [Abstract]2023 May;299(5):104668. PMID: 37011862 -
J Virol
The oncolytic avian reovirus p17 protein triggers chaperone-mediated autophagy by modulating Hsp90 and the T-complex protein-1 ring complex chaperones and co-chaperones to activate the IKK/NF-κB signaling. [Abstract]2025 Dec 23;99(12):e0108925. PMID: 41334925 -
J Virol
2025 Jun 5:e0050225. PMID: 40470959 -
Microbiol Spectr
Heat Shock Protein Member 8 (HSPA8) Is Involved in Porcine Reproductive and Respiratory Syndrome Virus Attachment and Internalization. [Abstract]2022 Feb 23;10(1):e0186021. PMID: 35138165 -
Biochim Biophys Acta Mol Cell Res
Heat shock protein 70 regulates m6A modification in response to heat shock in esophageal squamous cell carcinoma. [Abstract]2025 Jul 24;1872(8):120027. PMID: 40714122 -
J Cell Sci
Stress-specific NONO interactomes reveal a key role of Hsp70 chaperone activity in regulation of paraspeckle formation. [Abstract]2025 Dec 19:jcs.264115. PMID: 41416371 -
Vet Res
Heat shock protein 70 enhances viral replication by stabilizing Senecavirus A nonstructural proteins L and 3D. [Abstract]2024 Dec 18;55(1):158. PMID: 39695881 -
Microb Pathog
HSP/HSC70 activity is required for Bombyx mori nucleopolyhedrovirus replication at the early infectious phase. [Abstract]2021 Apr;153:104647. PMID: 33246089 -
Front Physiol
Deacetylation of HSC70-4 Promotes Bombyx mori Nucleopolyhedrovirus Proliferation via Proteasome-Mediated Nuclear Import. [Abstract]2021 Feb 19;12:609674. PMID: 33679433 -
Pathogens
Proteomic Discovery of VEEV E2-Host Partner Interactions Identifies GRP78 Inhibitor HA15 as a Potential Therapeutic for Alphavirus Infections. [Abstract]2021 Mar 2;10(3):283. PMID: 33801554 -
Am J Physiol Gastrointest Liver Physiol
Dopamine D2 receptor activator quinpirole protects against trypsinogen activation during acute pancreatitis via upregulating HSP70. [Abstract]2020 Jun 1;318(6):G1000-G1012. PMID: 32308041 -
Mol Biol Cell
HSPA8 inhibitors augment cancer chemotherapeutic effectiveness via potentiating necroptosis. [Abstract]2024 Jul 3:mbcE24040194. PMID: 38959101 -
Biochem Biophys Res Commun
2025 Sep 16:780:152466. PMID: 40829478 -
Domest Anim Endocrin
HSP70 inhibits pig pituitary gonadotrophin synthesis and secretion by regulating the corticotropin-releasing hormone signaling pathway and targeting SMAD3. [Abstract]2021 Jan;74:106533. PMID: 32992141 -
-
bioRxiv
2025 Jul 12:2025.07.08.663754. PMID: 40672312 -
bioRxiv
2025 Mar 26:2025.02.20.639340. PMID: 40060522 -
-
-
Solvent & Solubility
DMSO : 100 mg/mL (179.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.49 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.49 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
Embryos are removed from a pregnant ddY mouse at 14 days of gestation. Cells are treated with or without 10 μM Aβ25-35 for 3 days, followed by the addition of 0.05, 0.5, or 5 μM VER-155008 or vehicle solution (0.1% DMSO) for 4 days. The Aβ25-35 is incubated at 37°C for 4 days prior to treatment to facilitate aggregation. The cells are fixed with 4% paraformaldehyde and immunostained at 4°C for 24 h with antibodies against the axonal marker, mouse phosphorylated neurofilament heavy subunit, and against the neuronal marker, rabbit microtubule-associated protein 2. Alexa Fluor 488-conjugated goat anti-mouse IgG (1:400) and Alexa Fluor 568-conjugated goat anti-rabbit IgG (1:400) are used as secondary antibodies. Fluorescence images (864.98 μm × 645.62 μm) are captured using a fluorescence microscopy system. The lengths of the pNF-H-positive axons are measured using MetaMorph version 7.8[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Female BALB/c mice are dosed intravenously with 25 mg/kg VER-155008 into the lateral tail vein as a solution in 10% DMSO/5% Tween 80/85% saline (v/v/v). Animals are sacrificed at 5, 15 and 30 min, 1, 2, 4 and 6 h post dose[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
-
Data Sheet (286 KB)
-
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Portuguese - PT (392 KB)
-
Handling Instructions (2659 KB)
References
[1]. Massey AJ, et al. A novel, small molecule inhibitor of Hsc70/Hsp70 potentiates Hsp90 inhibitor induced apoptosis in HCT116 colon carcinoma cells. Cancer Chemother Pharmacol. 2010 Aug;66(3):535-45. [Content Brief]
[2]. Yang X, et al. Heat Shock Cognate 70 Inhibitor, VER-155008, Reduces Memory Deficits and Axonal Degeneration in a Mouse Model of Alzheimer's Disease. Front Pharmacol. 2018 Jan 30;9:48. [Content Brief]
[3]. Kita K, et al. Heat shock protein 70 inhibitors suppress androgen receptor expression in LNCaP95 prostate cancer cells. Cancer Sci. 2017 Sep;108(9):1820-1827. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7973 mL | 8.9863 mL | 17.9727 mL | 44.9317 mL |
| 5 mM | 0.3595 mL | 1.7973 mL | 3.5945 mL | 8.9863 mL | |
| 10 mM | 0.1797 mL | 0.8986 mL | 1.7973 mL | 4.4932 mL | |
| 15 mM | 0.1198 mL | 0.5991 mL | 1.1982 mL | 2.9954 mL | |
| 20 mM | 0.0899 mL | 0.4493 mL | 0.8986 mL | 2.2466 mL | |
| 25 mM | 0.0719 mL | 0.3595 mL | 0.7189 mL | 1.7973 mL | |
| 30 mM | 0.0599 mL | 0.2995 mL | 0.5991 mL | 1.4977 mL | |
| 40 mM | 0.0449 mL | 0.2247 mL | 0.4493 mL | 1.1233 mL | |
| 50 mM | 0.0359 mL | 0.1797 mL | 0.3595 mL | 0.8986 mL | |
| 60 mM | 0.0300 mL | 0.1498 mL | 0.2995 mL | 0.7489 mL | |
| 80 mM | 0.0225 mL | 0.1123 mL | 0.2247 mL | 0.5616 mL | |
| 100 mM | 0.0180 mL | 0.0899 mL | 0.1797 mL | 0.4493 mL |
Powered by Bioz