2. Apoptosis
  3. Caspase Apoptosis
  4. Ac-DEVD-CMK TFA

Ac-DEVD-CMK TFA  (Synonyms: Caspase-3 Inhibitor III TFA)

Cat. No.: HY-P0034A Purity: 99.07%
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Ac-DEVD-CMK (Caspase-3 Inhibitor III) TFA is a selective and irreversible caspase-3 inhibitor. Ac-DEVD-CMK TFA significantly inhibits apoptosis induced by high levels of glucose or 3,20-dibenzoate (IDB; HY-137295). Ac-DEVD-CMK TFA can be used in a variety of experimental approaches to inhibit apoptosis.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Ac-DEVD-CMK TFA Chemical Structure

Ac-DEVD-CMK TFA Chemical Structure

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1 mg USD 100 In-stock
5 mg USD 400 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Ac-DEVD-CMK TFA:

Ac-DEVD-CMK TFA Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Ac-DEVD-CMK TFA

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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  • Purity & Documentation

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  • Customer Review

Description

Ac-DEVD-CMK (Caspase-3 Inhibitor III) TFA is a selective and irreversible caspase-3 inhibitor. Ac-DEVD-CMK TFA significantly inhibits apoptosis induced by high levels of glucose or 3,20-dibenzoate (IDB; HY-137295). Ac-DEVD-CMK TFA can be used in a variety of experimental approaches to inhibit apoptosis[1][2][3].

In Vitro

Ac-DEVD-CMK TFA (100 μM; 24 h) inhibits IDB-induced apoptosis[3].
Ac-DEVD-CMK TFA inhibits (10 μM; 36 h) inhibits citrate (10 mM)-induced p21 cleavage and G2/M accumulation in human pharyngeal squamous carcinoma FaDu and Detroit 562 cell lines[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ac-DEVD-CMK TFA Related Antibodies

Western Blot Analysis[4]

Cell Line: FaDu and Detroit 562 cell
Concentration: 10 μM; with 10 mM citrate
Incubation Time: 36 h
Result: Inhibited citrate to induce p21 cleavage.
In Vivo

Ac-DEVD-CMK TFA (Caspase-3 Inhibitor III; 25 mg/kg; IP; single dose; 3 hours post-APAP) significantly attenuates Acetaminophen (APAP; HY-66005)-induced liver injury (AILI)[5].
Ac-DEVD-CMK TFA (25 mg/kg; ip; single dose) significantly attenuates APAP-induced liver injury (AILI) in susceptible Sdc1−/− mice[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Sdc1−/− mice induced by APAP[6]
Dosage: 25 mg/kg
Administration: IP; single dose, 3 hours post-APAP
Result: Inhibited GSK‐3β or caspase‐3 activity to mitigate liver damage.
Molecular Weight

664.97

Formula

C23H32ClF3N4O13
Appearance

Solid

Color

White to off-white

Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture and light, under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (150.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 50 mg/mL (75.19 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5038 mL 7.5191 mL 15.0383 mL
5 mM 0.3008 mL 1.5038 mL 3.0077 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.5038 mL 7.5191 mL 15.0383 mL 37.5957 mL
5 mM 0.3008 mL 1.5038 mL 3.0077 mL 7.5191 mL
10 mM 0.1504 mL 0.7519 mL 1.5038 mL 3.7596 mL
15 mM 0.1003 mL 0.5013 mL 1.0026 mL 2.5064 mL
20 mM 0.0752 mL 0.3760 mL 0.7519 mL 1.8798 mL
25 mM 0.0602 mL 0.3008 mL 0.6015 mL 1.5038 mL
30 mM 0.0501 mL 0.2506 mL 0.5013 mL 1.2532 mL
40 mM 0.0376 mL 0.1880 mL 0.3760 mL 0.9399 mL
50 mM 0.0301 mL 0.1504 mL 0.3008 mL 0.7519 mL
60 mM 0.0251 mL 0.1253 mL 0.2506 mL 0.6266 mL
DMSO 80 mM 0.0188 mL 0.0940 mL 0.1880 mL 0.4699 mL
100 mM 0.0150 mL 0.0752 mL 0.1504 mL 0.3760 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Ac-DEVD-CMK TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ac-DEVD-CMKCaspase-3 Inhibitor IIICaspaseApoptosisIrreversibleglucose,3,20-dibenzoateIDBInhibitorinhibitorinhibit

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