Ac-WEHD-AFC
Ac-WEHD-AFC is a fluorogenic caspase-1 substrate. Ac-WEHD-AFC can measure caspase-1 fluorogenic activity and can be used for the research of tumor and inflammation.
For research use only. We do not sell to patients.
- CAS No.: 210344-99-3
- Formula: C38H37F3N8O11
- Molecular Weight:838.74
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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Caspase-1 |
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Caspase-1 Fluorogenic Activity Assay[1]:
1. Incubate the cells according to your normal protocol.
2. Cells are transiently transfected with caspase-1 in combination with pcDNA3.1, Myc-cIAP2, Myc-cIAP1, or Myc-XIAP and collected 24 hr later.
3. Cells were lysed in CHEGG buffer before sonicating for 15 s at 60% amplitude (for cleavage assays).
4. Lysates were incubated with 10 mM of the fluorogenic caspase-1 substrate Ac-WEHD-AFC.
5. The release of free AFC was monitored continuously for 1 hr (excitation 380 nm, emission 460 nm) in 1 min intervals and expressed as arbitrary fluorescence units per minute.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 210344-99-3
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Molecular Weight 838.74
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Formula C38H37F3N8O11
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Sequence Shortening
Ac-WEHD-{AFC}
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Ac-WEHD-AFC Related Classifications
HY-P2617 Related Classifications
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