2. Epigenetics TGF-beta/Smad
  3. PKC
  4. Aprinocarsen sodium

Aprinocarsen sodium  (Synonyms: ISIS 3521 sodium)

Cat. No.: HY-148413 Purity: 90.35%
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Aprinocarsen (ISIS 3521) sodium, a specific antisense oligonucleotide inhibitor of protein kinase C-alpha (PKC-α). Aprinocarsen sodium is a 20-mer oligonucleotide, it regulates cell differentiation and proliferation. Aprinocarsen sodium inhibits the growth of human tumor cell lines in nude mice. Aprinocarsen sodium shows the value as a chemotherapeutic compound of human cancers.

For research use only. We do not sell to patients.

DNA, d(P-thio)(G-T-T-C-T-C-G-C-T-G-G-T-G-A-G-T-T-T-C-A), sodium salt (1:19)

Aprinocarsen sodium Chemical Structure

CAS No. : 331257-53-5

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1 mg USD 131 In-stock
5 mg USD 288 In-stock
10 mg USD 539 In-stock
25 mg USD 1078 In-stock
50 mg USD 1960 In-stock
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Based on 1 publication(s) in Google Scholar

Aprinocarsen sodium Related Antibodies

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PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKCι

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Description

Aprinocarsen (ISIS 3521) sodium, a specific antisense oligonucleotide inhibitor of protein kinase C-alpha (PKC-α). Aprinocarsen sodium is a 20-mer oligonucleotide, it regulates cell differentiation and proliferation. Aprinocarsen sodium inhibits the growth of human tumor cell lines in nude mice. Aprinocarsen sodium shows the value as a chemotherapeutic compound of human cancers[1].

In Vitro

Aprinocarsen sodium dose-dependently and oligonucleotide sequence specifically inhibits PKC-alpha in human bladder carcinoma (T-24) cells[1].
Aprinocarsen sodium shows an IC50 value of 50-100 nM for PKC-α mRNA reduction, but it shows no effect on the expression of other members of the PKC family of genes (PKC-eta and zeta)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Aprinocarsen sodium Related Antibodies
In Vivo

Aprinocarsen sodium (100 mg/kg; i.v.; at repeat doses for 14 days) shows well tolerated character with no apparently acute toxicity[1].
Aprinocarsen sodium (i.v.) dose-dependently inhibits the growth of T-24 bladder, human lung carcinoma (A549) and Colo 205 colon carcinoma human tumor cell lines in nude mice with ID50 values of 0.06-0.6 mg/kg daily[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

6852.85

Formula

C196H230N68Na19O105P19S19
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

DNA, d(P-thio)(G-T-T-C-T-C-G-C-T-G-G-T-G-A-G-T-T-T-C-A)
SMILES

[Aprinocarsen (sodium)]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (14.59 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.1459 mL 0.7296 mL 1.4592 mL
5 mM 0.0292 mL 0.1459 mL 0.2918 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.1459 mL 0.7296 mL 1.4592 mL 3.6481 mL
5 mM 0.0292 mL 0.1459 mL 0.2918 mL 0.7296 mL
10 mM 0.0146 mL 0.0730 mL 0.1459 mL 0.3648 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Aprinocarsen sodium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Aprinocarsen331257-53-5ISIS 3521ISIS3521ISIS-3521PKCProtein kinase Cacute toxicityT-24 bladderA549Colo 205 colon carcinomamiceInhibitorinhibitorinhibit

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