Heparan Sulfate
Based on 24 publication(s) in Google Scholar
Heparan sulfate, a complex and linear polysaccharide, exists as part of glycoproteins named heparan sulfate proteoglycans, which are expressed abundantly on the cell surface and in the extracellular matrix.
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- CAS. Nr.: 9050-30-0
- Formel: C12H19NO20S3 (monomer)
- Molecular Weight:593.47 (monomer)
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Speicherung:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Chemical Information
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EOC317FGFR/VEGFR/Tie-2 Inhibitor
Publications Citing Use of MedChemExpress (MCE) Heparan Sulfate
More- Immunity. 2021 May 11;54(5):962-975.e8. [Abstract]
- J Extracell Vesicles. 2026 Feb;15(2):e70238. [Abstract]
- Nat Commun. 2026 Jun 4. [Abstract]
- Nat Commun. 2026 Jun 9. [Abstract]
- Nat Commun. 2021 Jan 8;12(1):134. [Abstract]
- Sci Adv. 2023 Jun 2;9(22):eadg3365. [Abstract]
- Carbohydr Polym. 2019 Oct 15:222:115015. [Abstract]
- Carbohydr Polym. 2019 Jul 1:215:73-81. [Abstract]
- Carbohydr Polym. 2019 Mar 1:207:191-199. [Abstract]
- Int J Biol Macromol. 2020 Oct 15:161:1526-1534. [Abstract]
- Carbohydrate Polymer Technologies and Applications. 2025 Dec 19.
- J Dent Res. 2023 Feb;102(2):207-216. [Abstract]
- Biomolecules. 2024 Oct 19;14(10):1331. [Abstract]
- Front Cell Dev Biol. 2021 Sep 21:9:728352. [Abstract]
- J Thromb Haemost. 2021 Feb;19(2):470-477. [Abstract]
- Microbiol Spectr. 2025 Nov 13:e0234125. [Abstract]
- J Endod. 2024 Aug;50(8):1108-1116. [Abstract]
- Cell Mol Bioeng. 2026 Sep 22.
- Antibodies. 2026 Aug;15(4):52.
- J Clin Med. 2023 Oct 10;12(20):6438. [Abstract]
- Vet Microbiol. 2025 Jun:305:110518. [Abstract]
- Biochemistry. 2025 Jul 1;64(13):2737-2744. [Abstract]
- Biotechnol Appl Biochem. 2025 Feb;72(1):5-16. [Abstract]
- bioRxiv. 2026 Mar 5:2026.03.04.709643. [Abstract]
Customer Validation & Images
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RT-PCR
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Cell Proliferation/Viability Assay
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2D/3D Cell Culture and Differentiation
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Histological Imaging/Staining
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RT-PCR
Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen
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Biologische Aktivität
Beschreibung
IC50 & Target
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Human Endogenous Metabolite |
In Vitro
Heparan sulfate is a linear polysaccharide and belongs to the family of glycosaminoglycans. Heparan sulfate is composed of glucuronic acid (GlcA) and iduronic acid (IdoA) residues as well as N-acetyl glucosamines (GlcNAc) with various sulfation modifications, and is typically 50-200 disaccharides in length. Heparan sulfate interacts with numerous proteins, including growth factors, morphogens, and adhesion molecules, and thereby regulates important developmental processes in invertebrates and vertebrates. Heparan sulfate chains regulate developmental signaling by acting as co-factors through a variety of mechanisms that include but are not limited to maintenance of morphogen gradients and co-receptor functions[1]. Heparan sulfate proteoglycans can act as receptors for proteases and protease inhibitors regulating their spatial distribution and activity. Membrane Heparan sulfate proteoglycans act as coreceptors for various tyrosine kinase-type growth factor receptors, lowering their activation threshold or changing the duration of signaling reactions[2]. Heparan sulfate influences the binding affinity of intestinal epithelium cells to Wnt, thereby promoting activation of canonical Wnt signaling and facilitating regeneration of small intestinal crypts after epithelial injury[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 9050-30-0
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Appearance Solid
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Molecular Weight 593.47 (monomer)
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Formel C12H19NO20S3 (monomer)
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Color White to off-white
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SMILES
O=S(OCC1O[C@@H](OC)[C@H](NS(=O)(O)=O)[C@@H](OS(=O)(O)=O)[C@H]1O[C@@H]2OC(C(O)=O)[C@H](O[C@@H]3OC(COS(=O)(O)=O)[C@H](O[C@@H]4OC(C(O)=O)[C@H](OC)[C@H](O)[C@H]4OS(=O)(O)=O)[C@H](O)[C@H]3NS(=O)(O)=O)[C@H](O)[C@H]2O)(O)=O.[n]
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Structure Classification
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Initial Source
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (24)
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Journal Impact Factor
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Most Recent
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Immunity
Golgi apparatus-synthesized sulfated glycosaminoglycans mediate polymerization and activation of the cGAMP sensor STING. [Abstract]2021 May 11;54(5):962-975.e8. PMID: 33857420 -
J Extracell Vesicles
Extracellular Vesicles Coordinate Bacterial Cloaking in Lung Epithelial Cells to Alleviate Acute Inflammatory Injury. [Abstract]2026 Feb;15(2):e70238. PMID: 41656978 -
Nat Commun
GPI‑anchored protein nanoclusters license migrasome expansion and serve as exocytic platforms for migrasome. [Abstract]2026 Jun 4. PMID: 42243090 -
Nat Commun
2026 Jun 9. PMID: 42259798 -
Nat Commun
2021 Jan 8;12(1):134. PMID: 33420022
Heparan Sulfate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2021 Jan 8;12(1):134. [Abstract]
Calu3 and Caco2 cells were inoculated with Heparan Sulfate (100-500 μg/mL)-pretreated SARS-CoV-2 or SARS-CoV at 0.2 MOI for 2 h at 37 °C. Cell lysates and supernatants were harvested at 24hpi and virus replication was determined with qRT-PCR (n =3).
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Sci Adv
Surficial nano-deposition locoregionally yielding bactericidal super CAR-macrophages expedites periprosthetic osseointegration. [Abstract]2023 Jun 2;9(22):eadg3365. PMID: 37256944 -
Carbohydr Polym
Co-production of chondroitin sulfate and peptide from liquefied chicken sternal cartilage by hot-pressure. [Abstract]2019 Oct 15:222:115015. PMID: 31320074 -
Carbohydr Polym
Liquefaction of chicken sternal cartilage by steam explosion to isolate chondroitin sulfate. [Abstract]2019 Jul 1:215:73-81. PMID: 30981372 -
Carbohydr Polym
2019 Mar 1:207:191-199. PMID: 30599999 -
Int J Biol Macromol
Selective regulation of RANKL/RANK/OPG pathway by heparan sulfate through the binding with estrogen receptor β in MC3T3-E1 cells. [Abstract]2020 Oct 15:161:1526-1534. PMID: 32771510 -
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J Dent Res
2023 Feb;102(2):207-216. PMID: 36281071 -
Biomolecules
A Novel Peptide from VP1 of EV-D68 Exhibits Broad-Spectrum Antiviral Activity Against Human Enteroviruses. [Abstract]2024 Oct 19;14(10):1331. PMID: 39456264
Heparan Sulfate purchased from MedChemExpress. Usage Cited in: Biomolecules. 2024 Oct 19;14(10):1331. [Abstract]
To confirm the interaction of P25 with receptor Heparan Sulfate (HS) on the cell surface, 50 µL P25 or its derivatives at a concentration of 62.5 µg/mL were pre-incubated with 50 µL cell-free HS at different concentrations of 0–0.5 mg/mL at 37 °C for 1 h, mixed with 100 TCID50/50 µL of EV-A71/FY0805, and infected the pre-seeding RD monolayer cells on 96-well plates for 24 h. The CPE was detected and stained using crystal violet. The optical density (OD) at 550 nm was read using the TECAN Infinite 200 PRO instrument.The results showed that HS diminished the infection inhibitions of P25s.
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Front Cell Dev Biol
Cochlear Sox2+ Glial Cells Are Potent Progenitors for Spiral Ganglion Neuron Reprogramming Induced by Small Molecules. [Abstract]2021 Sep 21:9:728352. PMID: 34621745 -
J Thromb Haemost
Skeletal muscle myosin and cardiac myosin attenuate heparin's antithrombin-dependent anticoagulant activity. [Abstract]2021 Feb;19(2):470-477. PMID: 33176060 -
Microbiol Spectr
New insight into the epidemiological trends of respiratory syncytial virus infection and the underlying anti-respiratory syncytial virus mechanisms of andrographolide: integrating Global Burden of Disease database, network pharmacological analysis, and in vitro experiments. [Abstract]2025 Nov 13:e0234125. PMID: 41230964
Heparan Sulfate purchased from MedChemExpress. Usage Cited in: Microbiol Spectr. 2025 Nov 13:e0234125. [Abstract]
Virus load experiment on RSV F protein gene expression treated with andrographolide (12.5 µM; 26.5 h), Heparan Sulfate (HS, 5 mg/mL; 26.5 h), or their combination.
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J Endod
Effect of Heparan Sulfate on Vasculogenesis and Dentinogenesis of Dental Pulp Stem Cells. [Abstract]2024 Aug;50(8):1108-1116. PMID: 38719089
Heparan Sulfate purchased from MedChemExpress. Usage Cited in: J Endod. 2024 Aug;50(8):1108-1116. [Abstract]
The effect of Heparan Sulfate (HS) on proliferation and endothelial differentiation of DPSCs. Photomicrographs of DPSCs cultured in EM containing different concentrations of Heparan Sulfate (HS, 0.01-100 μg/mL) for up to 5 days. Scale bars: 200 μm.
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J Clin Med
The Effects of Heparan Sulfate Infusion on Endothelial and Organ Injury in a Rat Pneumosepsis Model. [Abstract]2023 Oct 10;12(20):6438. PMID: 37892576
Heparan Sulfate purchased from MedChemExpress. Usage Cited in: J Clin Med. 2023 Oct 10;12(20):6438. [Abstract]
H&E staining of representative lung tissue in sham, RL, RL + Heparan Sulfate (HS, 7 mg/kg; administered via a perfusion pump) , albu, and albu + HS groups.
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Vet Microbiol
Surface expression of PEDV nucleocapsid protein facilitates NK cell-mediated ADCC via anti-N nonneutralizing antibody. [Abstract]2025 Jun:305:110518. PMID: 40233683 -
Biochemistry
The Crystal Structure of Acanthamoeba Polyphaga Mimivirus R655 and Its Potential Glycosaminoglycan Glycosyltransferase Activity. [Abstract]2025 Jul 1;64(13):2737-2744. PMID: 40545624 -
Biotechnol Appl Biochem
Characterization of maltose-binding protein-fused heparinases with enhanced thermostability by application of rigid and flexible linkers. [Abstract]2025 Feb;72(1):5-16. PMID: 39072851 -
bioRxiv
Comparative Genomic and Functional Profiling of ECM-Targeting Enzymes in Bacteroides, a Key Genus of the Human Gut Microbiome. [Abstract]2026 Mar 5:2026.03.04.709643. PMID: 41847005
Lösungsmittel & Löslichkeit
In Vitro:
H2O : 100 mg/mL (Need ultrasonic)
In Vivo:
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL; Clear solution; Need ultrasonic
Protokoll
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Fibrosis/Collagen Morphometry
Fibrosis and collagen morphometry is based on the quantitative visualization of fibrillar collagen deposition in tissue sections using histochemical stains such as Sirius Red (Picrosirius Red) or Masson's trichrome, followed by image-based or polarization-enhanced analysis to estimate collagen proportional area as a surrogate of extracellular matrix accumulation during fibrotic remodeling. Sirius Red combined with polarized light microscopy enhances detection of collagen fibers due to birefringence properties, enabling more specific visualization of collagen type I and III fibrils compared to conventional bright-field histology, while whole-section or region-restricted digital morphometry reduces field-selection bias in fibrosis assessment. Alternative quantitative approaches include second harmonic generation (SHG) and two-photon excited fluorescence microscopy, which enable label-free detection of fibrillar collagen and have been validated against histological staining and biochemica
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Collagen: Sirius Red Staining
Sirius Red or picrosirius red staining is a histochemical method for visualizing collagen-rich extracellular matrix in tissue sections, and collagen fibers are detected as red-stained structures under bright-field microscopy with enhanced birefringence under polarized light. Picrosirius red is useful for assessing total collagen organization, distribution, and fibrosis burden, but polarized color should not be interpreted as a definitive collagen type I versus type III readout because color is affected by fiber orientation, thickness, and packing.
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ECM-Embedded Organoid (Matrigel/Dome) Culture
ECM-embedded organoid dome culture embeds epithelial stem cells, crypts, organoid fragments, or tumor-derived epithelial cells in a basement-membrane-like hydrogel such as Matrigel, allowing 3D growth, self-organization, lumen formation, budding or cystic morphogenesis, and lineage maintenance under defined niche-factor-containing medium. The primary readouts are organoid establishment efficiency, growth, morphology, passaging capacity, lineage-marker expression, and, when fluorescently labeled lines are used, microscopy- or flow-cytometry-based quantification of population behavior in 3D culture.
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Matrigel/ECM Transwell Invasion Assay
The Matrigel/ECM Transwell invasion assay measures the ability of cells to move toward a chemoattractant while crossing an extracellular-matrix barrier placed on a porous membrane; therefore, the readout reflects both chemotactic motility and matrix invasion rather than migration alone. Matrigel is a basement-membrane-rich matrix derived from Engelbreth-Holm-Swarm mouse sarcoma and has been used as a reconstituted basement membrane barrier in chemoinvasion assays. The assay readout is generated by quantifying cells that reach the underside of the insert membrane or lower compartment after incubation, commonly by staining and counting invaded cells or by fluorescence-based quantification.
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Matrigel Transwell/Boyden Chamber Invasion Assay
Matrigel Transwell/Boyden chamber invasion assay measures the ability of cells to degrade or traverse an extracellular matrix-coated porous membrane and move from an upper chamber toward a chemoattractant in a lower chamber. Invasion is distinguished from migration by coating the membrane with Matrigel or basement membrane matrix; uncoated inserts measure migration, while coated inserts require cells to cross an ECM barrier before reaching the underside of the membrane.
Reinheit & Dokumentation
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Data Sheet (282 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Kraushaar DC, et al. Heparan sulfate: a key regulator of embryonic stem cell fate. Biol Chem. 2013 Jun;394(6):741-51. [Content Brief]
[2]. Sarrazin S, et al. Heparan sulfate proteoglycans. Cold Spring Harb Perspect Biol. 2011 Jul 1;3(7). pii: a004952. [Content Brief]
[3]. Yamamoto S, et al. Heparan sulfate on intestinal epithelial cells plays a critical role in intestinal crypt homeostasis via Wnt/β-catenin signaling. Am J Physiol Gastrointest Liver Physiol. 2013 Aug 1;305(3):G241-9. [Content Brief]
[4]. Minge D, et al. Heparan Sulfates Support Pyramidal Cell Excitability, Synaptic Plasticity, and Context Discrimination. Cereb Cortex. 2017 Feb 1;27(2):903-918. [Content Brief]
[5]. Gómez-Pinilla F, et al. Regulation of astrocyte proliferation by FGF-2 and heparan sulfate in vivo. J Neurosci. 1995 Mar;15(3 Pt 1):2021-9. [Content Brief]
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Heparan Sulfate Related Classifications
HY-101916 Related Classifications
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