2. Epigenetics
    Stem Cell/Wnt
    Protein Tyrosine Kinase/RTK
    JAK/STAT Signaling
  3. JAK
    STAT
  4. BD750

BD750 

Cat. No.: HY-131140 Purity: 99.79%
COA Handling Instructions

BD750, an effective immunosuppressant and a JAK3/STAT5 inhibitor, inhibits IL-2-induced JAK3/STAT5-dependent T cell proliferation, with IC50 values of 1.5 μM and 1.1 μM in mouse and human T cells, respectively.

For research use only. We do not sell to patients.

BD750 Chemical Structure

BD750 Chemical Structure

CAS No. : 892686-59-8

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 66 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 60 In-stock
Estimated Time of Arrival: December 31
10 mg USD 100 In-stock
Estimated Time of Arrival: December 31
25 mg USD 180 In-stock
Estimated Time of Arrival: December 31
50 mg USD 300 In-stock
Estimated Time of Arrival: December 31
100 mg USD 500 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 3 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE BD750

Cell Viability Assay
WB

    BD750 purchased from MCE. Usage Cited in: FASEB J. 2023 Jan;37(1):e22716.  [Abstract]

    BD750 (24 h) successfully inhibits the JAK–STAT signaling pathway in AML-12 cells. The relevant protein expression levels of the JAK–STAT signaling pathway are detected after transfection of NC-siRNA, P38γ-siRNA, and pEGFP-C1, pEGFP-C1-P38γ and co-cultured with BD750 for 24 h in FFA-treated AML-12 cells.

    BD750 purchased from MCE. Usage Cited in: FASEB J. 2023 Jan;37(1):e22716.  [Abstract]

    BD750 (0, 2.5, 5, 10, 20, 40 μM; 24 h) decreases AML-12 cells vitality in a concentration-dependent manner.

    View All JAK Isoform Specific Products:

    View All Isoforms
    JAK1 JAK2 JAK3 Tyk2 JAK

    View All STAT Isoform Specific Products:

    View All Isoforms
    STAT3 STAT5 STAT6 STAT1

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    BD750, an effective immunosuppressant and a JAK3/STAT5 inhibitor, inhibits IL-2-induced JAK3/STAT5-dependent T cell proliferation, with IC50 values of 1.5 μM and 1.1 μM in mouse and human T cells, respectively[1][2].

    IC50 & Target[2]

    JAK3

     

    STAT5

     

    In Vitro

    BD750 inhibits human T cell proliferation stimulated either by anti-CD3/anti-CD28 mAbs or by alloantigen in a dose-dependent manner with IC50 values of 1.1 ± 0.2 μM and 1.3 ± 0.2 μM respectively[1].
    BD750 also inhibits ConA, PMA/ionomycine or alloantigen-induced mouse T cell proliferation and PHA or PMA/ionomycine-induced human T cell proliferation[1].
    BD750 (5 or 20 μM) inhibits the LPS-induced JAK-STAT5 signaling in DC[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Western Blot Analysis[2]

    Cell Line: Purified immature DCs.
    Concentration: 1, 5 or 20 μM.
    Incubation Time: 12 h.
    Result: Had fewer small dendrites and smaller clusters than typical mDCs (5 or 20 μM).
    At a higher dose significantly reduced the levels of LPS-stimulated IL-6, IL-12, TNF-α, IL-1β and IL-23 production by DCs.
    In Vivo

    BD750 can induce tolerogenic dendritic cells (tolDC) and their function in experimental autoimmune encephalitis (EAE) in mice[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Female C57BL/6 mice (10 wks old, 19-21 g)[2].
    Dosage: 20 μM.
    Administration: IV, treated DC on d 7, 11 and 15 post the first PTX injection (dpi 7, 11 and 15).
    Result: Significantly reduced the frequency of Th1 and Th17 cells and increased the percentage of Tregs compared with mice receiving PBS.
    Molecular Weight

    271.34

    Appearance

    Solid

    Formula

    C14H13N3OS
    CAS No.
    SMILES

    OC1=C2CCCCC2=NN1C3=NC4=CC=CC=C4S3
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 125 mg/mL (460.68 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.6854 mL 18.4271 mL 36.8541 mL
    5 mM 0.7371 mL 3.6854 mL 7.3708 mL
    10 mM 0.3685 mL 1.8427 mL 3.6854 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.08 mg/mL (7.67 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation
    References
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Keywords:

    BD750892686-59-8BD 750BD-750JAKSTATJanus kinaseTcellImmunosuppressantInhibitorinhibitorinhibit

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