AICAR
Based on 216 publication(s) in Google Scholar
AICAR (Acadesine) is an adenosine analog and a AMPK activator. AICAR is also an autophagy, YAP and mitophagy inhibitor. AICAR regulates the glucose and lipid metabolism, and inhibits proinflammatory cytokines and iNOS production. AICAR inhibits autophagy through a mechanism independent of AMPK activity.
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- Pureté: 99.99%
- CAS No.: 2627-69-2
- Formule: C9H14N4O5
- Masse moléculaire:258.23
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Publications Citing Use of MedChemExpress (MCE) AICAR
More- Signal Transduct Target Ther. 2025 Sep 26;10(1):315. [Abstract]
- Signal Transduct Target Ther. 2023 Mar 6;8(1):95. [Abstract]
- Signal Transduct Target Ther. 2022 Feb 14;7(1):40. [Abstract]
- Nature. 2025 Feb;638(8052):1112-1121. [Abstract]
- Cancer Cell. 2020 Sep 14;38(3):350-365.e7. [Abstract]
- Cell Metab. 2025 Jun 3;37(6):1364-1380.e6. [Abstract]
- Cell Metab. 2019 Jul 2;30(1):157-173.e7 [Abstract]
- Cancer Res. 2025 Mar 14;85(6):1113-1129. [Abstract]
- Cancer Res. 2023 Dec 15;83(24):4063-4079. [Abstract]
- ACS Nano. 2025 Aug 26;19(33):30441-30454. [Abstract]
- Cell Death Differ. 2025 May 22. [Abstract]
- Cell Death Differ. 2021 Apr;28(4):1222-1236. [Abstract]
- Adv Sci (Weinh). 2025 Dec 5:e20444. [Abstract]
- Adv Sci (Weinh). 2025 Dec 22:e13400. [Abstract]
- Chem Eng J. 2025 Sep 15.
- J Exp Clin Cancer Res. 2021 Jun 23;40(1):206. [Abstract]
- J Nanobiotechnology. 2025 Jul 5;23(1):486. [Abstract]
- Redox Biol. 2026 Apr:91:104088. [Abstract]
- Redox Biol. 2021 Oct:46:102082. [Abstract]
- Gut Microbes. 2024 Jan-Dec;16(1):2412381. [Abstract]
- Pharmacol Res. 2023 Feb:188:106666. [Abstract]
- Pharmacol Res. 2022 Jan:175:105985. [Abstract]
- Pharmacol Res. 2021 May:167:105561. [Abstract]
- Cell Mol Biol Lett. 2022 Aug 9;27(1):66. [Abstract]
- Cancer Lett. 2026 Apr 28:644:218311. [Abstract]
- Cancer Lett. 2025 May 23:217810. [Abstract]
- Int J Biol Sci. 2024 Aug 19;20(11):4476-4495. [Abstract]
- Int J Biol Sci. 2021 Jul 22;17(12):3091-3103. [Abstract]
- Cell Death Dis. 2025 Feb 3;16(1):66. [Abstract]
- Cell Death Dis. 2024 Sep 30;15(9):697. [Abstract]
- Cell Death Dis. 2021 May 18;12(6):506. [Abstract]
- Cell Death Dis. 2018 May 1;9(5):515. [Abstract]
- Proc Natl Acad Sci U S A. 2024 Oct 29;121(44):e2321305121. [Abstract]
- Cell Commun Signal. 2024 Nov 7;22(1):534. [Abstract]
- Cell Commun Signal. 2023 May 4;21(1):99. [Abstract]
- Int J Biol Macromol. 2025 Oct 12;330(Pt 4):148291. [Abstract]
- Int J Biol Macromol. 2025 Sep;321(Pt 4):146448. [Abstract]
- Int J Biol Macromol. 2024 Feb 5;262(Pt 1):129950. [Abstract]
- Int J Biol Macromol. 2023 Aug 30:247:125816. [Abstract]
- Phytomedicine. 2024 Dec:135:156111. [Abstract]
- Phytomedicine. 2024 Jul:129:155534. [Abstract]
- Phytomedicine. 2021 May:85:153526. [Abstract]
- NPJ Parkinsons Dis. 2025 May 6;11(1):114. [Abstract]
- Free Radic Biol Med. 2025 Apr 25:S0891-5849(25)00250-3. [Abstract]
- Free Radic Biol Med. 2023 Feb 1:195:89-102. [Abstract]
- Regen Biomater. 2024 Mar 1:11:rbae020. [Abstract]
- Food Res Int. 2025 Dec;221(Pt 3):117392. [Abstract]
- Clin Sci. 2025 Oct 22;139(20):1163-1185. [Abstract]
- Br J Pharmacol. 2025 May 13. [Abstract]
- Alzheimers Res Ther. 2025 Jun 23;17(1):141. [Abstract]
- Biomed Pharmacother. 2023 Aug:164:114907. [Abstract]
- Europace. 2025 Oct 31;27(11):euaf288. [Abstract]
- Stem Cell Res Ther. 2025 Apr 24;16(1):206. [Abstract]
- Environ Pollut. 2023 Feb 7;322:121202. [Abstract]
- Oncogene. 2019 Sep;38(38):6537-6549. [Abstract]
- Cell Chem Biol. 2026 May 21;33(5):683-698.e7. [Abstract]
- Curr Res Food Sci. 2024 Mar 28:8:100728. [Abstract]
- Cell Death Discov. 2024 Jan 17;10(1):34. [Abstract]
- Cell Rep. 2022 Nov 22;41(8):111707. [Abstract]
- Cell Rep. 2022 Aug 16;40(7):111204. [Abstract]
- Cell Rep. 2021 Sep 21;36(12):109739. [Abstract]
- Arch Toxicol. 2019 Jun;93(6):1697-1712. [Abstract]
- Br J Cancer. 2023 May;128(9):1647-1664. [Abstract]
- Clin Transl Med. 2022 Mar;12(3):e777. [Abstract]
- Clin Transl Med. 2020 Oct;10(6):e205. [Abstract]
- Neural Regen Res. 2026 Jan 1;21(1):377-391. [Abstract]
- J Anim Sci Biotechnol. 2026 Feb 12;17(1):29. [Abstract]
- J Anim Sci Biotechnol. 2023 Nov 2;14(1):141. [Abstract]
- Phytother Res. 2019 Jul;33(7):1794-1804. [Abstract]
- J Agric Food Chem. 2025 Dec 22. [Abstract]
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- J Agric Food Chem. 2024 May 8;72(18):10339-10354. [Abstract]
- J Agric Food Chem. 2022 Sep 14;70(36):11324-11335. [Abstract]
- J Agric Food Chem. 2021 Aug 11;69(31):8714-8725. [Abstract]
- J Agric Food Chem. 2019 Jun 26;67(25):7060-7072. [Abstract]
- Ecotoxicol Environ Saf. 2024 Jan 15:270:115831. [Abstract]
- Antioxid Redox Signal. 2024 Jan;40(1-3):16-39. [Abstract]
- JCI Insight. 2022 Mar 8;7(5):e154595. [Abstract]
- Cell Biol Toxicol. 2025 Feb 3;41(1):39. [Abstract]
- Front Immunol. 2021 Nov 8:12:753681. [Abstract]
- Int J Mol Med. 2025 Feb;55(2):26. [Abstract]
- Int J Mol Med. 2023 Apr;51(4):34. [Abstract]
- Int J Mol Med. 2018 Aug;42(2):799-810. [Abstract]
- Int J Mol Med. 2018 May;41(5):2535-2544. [Abstract]
- Chin Med. 2026 Mar 1;21(1):74. [Abstract]
- Chin Med. 2025 Jul 7;20(1):108. [Abstract]
- Biochem Pharmacol. 2025 Jul 12:241:117162. [Abstract]
- Am J Chin Med. 2020;48(7):1693-1713. [Abstract]
- Food Funct. 2026 Apr 14;17(7):3172-3185. [Abstract]
- J Ethnopharmacol. 2026 Apr 6:360:121151. [Abstract]
- J Ethnopharmacol. 2024 Jan 30;319(Pt 1):117162. [Abstract]
- J Ethnopharmacol. 2023 Dec 5:317:116731. [Abstract]
- Food Funct. 2020 Apr 1;11(4):3073-3083. [Abstract]
- Arab J Chem. 2024 Aug.
- Cells. 2022 Aug 30;11(17):2706. [Abstract]
- Commun Biol. 2026 Mar 10;9(1):566. [Abstract]
- Commun Biol. 2025 May 30;8(1):830. [Abstract]
- Nutrients. 2021 May 21;13(6):1740. [Abstract]
- Nutrients. 2020 Oct 19;12(10):3195. [Abstract]
- J Affect Disord. 2026 Jun 15:403:121439. [Abstract]
- Int J Mol Sci. 2025 Feb 11;26(4):1521. [Abstract]
- Front Pharmacol. 2025 Mar 26:16:1527316. [Abstract]
- Front Pharmacol. 2023 Apr 18:14:1148611. [Abstract]
- Front Pharmacol. 2022 May 13;13:887497. [Abstract]
- Front Pharmacol. 2021 Jul 7:12:687394. [Abstract]
- Front Pharmacol. 2020 Jul 31;11:1038. [Abstract]
- Front Pharmacol. 2018 Jul 16:9:761. [Abstract]
- Front Pharmacol. 2018 Apr 16:9:345. [Abstract]
- Int Immunopharmacol. 2026 Apr 15:175:116446. [Abstract]
- mBio. 2025 Aug 13;16(8):e0097625. [Abstract]
- Eur J Pharmacol. 2024 Jun 5:972:176543. [Abstract]
- Int Immunopharmacol. 2022 Oct:111:109165. [Abstract]
- Int Immunopharmacol. 2022 Oct:111:109114. [Abstract]
- Int Immunopharmacol. 2022 May:106:108600. [Abstract]
- Neuropharmacology. 2024 Nov 1:110206. [Abstract]
- Front Microbiol. 2020 Feb 28;11:320. [Abstract]
- FASEB J. 2026 Mar 31;40(6):e71712. [Abstract]
- Poult Sci. 2026 Feb 1;105(4):106573. [Abstract]
- Exp Neurol. 2025 Feb 2:386:115174. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2024 Jun 25:167314. [Abstract]
- Mol Nutr Food Res. 2024 Jun 11:e2300915. [Abstract]
- J Cell Mol Med. 2023 Apr;27(8):1069-1082. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2022 May 1;1868(5):166369. [Abstract]
- J Cell Mol Med. 2022 Feb;26(3):893-912. [Abstract]
- FASEB J. 2020 Nov;34(11):14946-14959. [Abstract]
- J Cell Mol Med. 2020 Aug;24(16):9135-9144. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2018 Nov;1864(11):3723-3738. [Abstract]
- iScience. 2023 Jul 12;26(8):107295. [Abstract]
- Biomed Environ Sci. 2019 Dec;32(12):944-948. [Abstract]
- Antiviral Res. 2025 Jan 16:235:106081. [Abstract]
- J Funct Foods. 2024 May, 116, 106168.
- Antiviral Res. 2023 Mar:211:105542. [Abstract]
- J Integr Med. 2022 Jul;20(4):365-375. [Abstract]
- J Cell Physiol. 2018 Dec;233(12):9701-9715. [Abstract]
- Sci Rep. 2025 Mar 13;15(1):8689. [Abstract]
- Aquac Nutr. 2025 Feb 26:2025:9406490. [Abstract]
- Aquaculture. 2025 Jan 15.
- J Biol Chem. 2024 Jul 11:107549. [Abstract]
- J Biol Chem. 2022 Dec;298(12):102587. [Abstract]
- Biomedicines. 2022 Jan 13;10(1):165. [Abstract]
- Neurochem Res. 2025 Jun 5;50(3):180. [Abstract]
- J Virol. 2024 Mar 19;98(3):e0175123. [Abstract]
- Biochim Biophys Acta Mol Cell Res. 2026 Mar;1873(3):120111. [Abstract]
- Cell Signal. 2025 Nov:135:111985. [Abstract]
- Cardiovasc Toxicol. 2024 Sep;24(9):889-903. [Abstract]
- Cell Signal. 2024 Jun:118:111125. [Abstract]
- Aquac Rep. 2023 Jun, 30, 101549.
- Cell Signal. 2023 Jan:101:110502. [Abstract]
- Biochim Biophys Acta Mol Cell Res. 2022 Aug;1869(8):119265. [Abstract]
- Enzyme Microb Technol. 2021 Mar:144:109737. [Abstract]
- J Photochem Photobiol B. 2020 Dec;213:112075. [Abstract]
- Mol Cell Biochem. 2020 Aug;471(1-2):91-100. [Abstract]
- Mol Cell Endocrinol. 2026 May:615:112739. [Abstract]
- Mol Cell Endocrinol. 2025 Jan 21:599:112464. [Abstract]
- Heliyon. 2023 Nov 28;9(12):e22939. [Abstract]
- J Immunol Res. 2022 Feb 8;2022:2619781. [Abstract]
- Food Chem Toxicol. 2024 Jun 5:114790. [Abstract]
- Med Oncol. 2023 Dec 26;41(1):29. [Abstract]
- Food Chem Toxicol. 2023 Nov:181:114089. [Abstract]
- Toxicol Appl Pharmacol. 2026 Mar 14:511:117797. [Abstract]
- J Immunol. 2022 Dec 1;209(11):2181-2191. [Abstract]
- Front Physiol. 2021 Oct 27:12:745058. [Abstract]
- Acta Biochim Biophys Sin (Shanghai). 2021 Jan 12;53(1):112-118. [Abstract]
- Acta Biochim Biophys Sin (Shanghai). 2018 Feb 1;50(2):144-155. [Abstract]
- Biochim Biophys Acta Mol Cell Biol Lipids. 2024 Mar;1869(2):159428. [Abstract]
- Cell Biol Int. 2024 Jan;48(1):46-59. [Abstract]
- Cell Biol Int. 2023 Nov;47(11):1813-1824. [Abstract]
- Adipocyte. 2022 Dec;11(1):562-571. [Abstract]
- J Leukoc Biol. 2022 Aug;112(2):299-311. [Abstract]
- Curr Res Toxicol. 2026 Mar 10:10:100288. [Abstract]
- Curr Issues Mol Biol. 2025 Nov 19;47(11):963. [Abstract]
- Arch Biochem Biophys. 2023 Aug:744:109689. [Abstract]
- J Orthop Surg Res. 2023 Jun 27;18(1):462. [Abstract]
- Genes (Basel). 2022 Dec 16;13(12):2384. [Abstract]
- Mol Biol Rep. 2019 Dec;46(6):6435-6451. [Abstract]
- Exp Eye Res. 2024 Apr:241:109851. [Abstract]
- Exp Eye Res. 2020 Apr;193:107972. [Abstract]
- Tissue Cell. 2025 Oct 14:98:103186. [Abstract]
- Theriogenology. 2024 May 24:225:152-161. [Abstract]
- Acta Cardiol. 2023 Jul;78(5):594-606. [Abstract]
- Exp Ther Med. 2022 Jun 1;24(1):485. [Abstract]
- Biochem Biophys Res Commun. 2023 Oct 1:675:54-60. [Abstract]
- J Cardiovasc Pharmacol. 2022 May 1;79(5):730-738. [Abstract]
- Connect Tissue Res. 2025 Feb 11:1-14. [Abstract]
- Endocr J. 2022 Apr 28;69(4):385-397. [Abstract]
- Curr Eye Res. 2025 Jun;50(6):600-609. [Abstract]
- Curr Eye Res. 2022 May 25;1-13. [Abstract]
- Virus Genes. 2026 Jan 5. [Abstract]
- Drug Chem Toxicol. 2026 Jan 3:1-12. [Abstract]
- Virus Genes. 2022 Apr;58(2):133-142. [Abstract]
- Nutr Res Pract. 2024 Aug;18(4):479-497. [Abstract]
- Cardiol Res. 2024 Oct;15(5):404-414. [Abstract]
- Res Sq. 2025 Nov 19.
- bioRxiv. 2025 Sep 29.
- University of Michigan. 2025.
- Justus Liebig University Giessen. 2025.
- bioRxiv. 2025 Aug 28:2025.08.28.672931. [Abstract]
- bioRxiv. 2025 Jul 12:2025.07.08.663754. [Abstract]
- SSRN. 2025 May 8.
- bioRxiv. 2025 May 05.
- SSRN. 2024 Sep 28.
- Res Sq. 2024 Jun 25.
- bioRxiv. 2024 May 25.
- Research Square Preprint. 2023 Jun 26.
- Herald of Medicine. 2023 Jun, 42(6): 781-789.
- Research Square Preprint. 2023 May 26.
- Research Square Print. 2023 Jan 24.
- Research Square Print. December 19th, 2022.
- Oxid Med Cell Longev. 2022 Oct 4:2022:2353115. [Abstract]
- Authorea. September 07, 2022.
- Oxid Med Cell Longev. 2022 Aug 5:2022:3982613. [Abstract]
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- J Oncol. 2021 Jul 14:2021:8281718. [Abstract]
- bioRxiv. 2021 Jan 31.
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Voir tous les produits spécifiques à Isoform AMPK
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Activité biologique
|
AMPK |
Autophagy |
Mitophagy |
Human Endogenous Metabolite |
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| CCRF-CEM | IC50 |
210 μM
Compound: 13, AICA
|
Cytostatic activity against human CEM cells assessed as growth inhibition after 3 days by particle counting analysis
Cytostatic activity against human CEM cells assessed as growth inhibition after 3 days by particle counting analysis
|
[PMID: 24177359] |
| HCT-116 | EC50 |
>100 μM
Compound: AICAR
|
Cytotoxicity against human HCT-116 cells incubated for 48 hrs by MTT assay
Cytotoxicity against human HCT-116 cells incubated for 48 hrs by MTT assay
|
[PMID: 36518704] |
| HCT-116 | EC50 |
>100 μM
Compound: AICAR
|
Cytotoxicity against human HCT-116 cells overexpressing human BCRP incubated for 48 hrs by MTT assay
Cytotoxicity against human HCT-116 cells overexpressing human BCRP incubated for 48 hrs by MTT assay
|
[PMID: 36518704] |
| HCT-116 | EC50 |
>100 μM
Compound: AICAR
|
Cytotoxicity against human HCT-116 cells overexpressing human MDR1 incubated for 48 hrs by MTT assay
Cytotoxicity against human HCT-116 cells overexpressing human MDR1 incubated for 48 hrs by MTT assay
|
[PMID: 36518704] |
| HEK293 | EC50 |
>100 μM
Compound: AICAR
|
Cytotoxicity against human HEK293 cells incubated for 48 hrs by MTT assay
Cytotoxicity against human HEK293 cells incubated for 48 hrs by MTT assay
|
[PMID: 36518704] |
| HEL | EC50 |
>100 μM
Compound: 13, AICA
|
Antiviral activity against acyclovir-resistant thymidine kinase-deficient Herpes simplex virus 1 KOS infected in HEL cells assessed as inhibition of virus-induced cytopathicity
Antiviral activity against acyclovir-resistant thymidine kinase-deficient Herpes simplex virus 1 KOS infected in HEL cells assessed as inhibition of virus-induced cytopathicity
|
[PMID: 24177359] |
| HEL | EC50 |
>100 μM
Compound: 13, AICA
|
Antiviral activity against Herpes simplex virus 1 KOS infected in HEL cells assessed as inhibition of virus-induced cytopathicity
Antiviral activity against Herpes simplex virus 1 KOS infected in HEL cells assessed as inhibition of virus-induced cytopathicity
|
[PMID: 24177359] |
| HEL | EC50 |
>100 μM
Compound: 13, AICA
|
Antiviral activity against Herpes simplex virus 2 G infected in HEL cells assessed as inhibition of virus-induced cytopathicity
Antiviral activity against Herpes simplex virus 2 G infected in HEL cells assessed as inhibition of virus-induced cytopathicity
|
[PMID: 24177359] |
| HeLa | IC50 |
165 μM
Compound: 13, AICA
|
Cytostatic activity against human HeLa cells assessed as growth inhibition after 3 days by particle counting analysis
Cytostatic activity against human HeLa cells assessed as growth inhibition after 3 days by particle counting analysis
|
[PMID: 24177359] |
| HeLa | IC50 |
≥250 μM
Compound: 13, AICA
|
Cytostatic activity against thymidine kinase-deficient human HeLa cells assessed as growth inhibition after 3 days by particle counting analysis
Cytostatic activity against thymidine kinase-deficient human HeLa cells assessed as growth inhibition after 3 days by particle counting analysis
|
[PMID: 24177359] |
| K562 | IC50 |
800 μM
Compound: Aca
|
Antileukemic activity against human K562 cells after 48 hrs by XTT assay
Antileukemic activity against human K562 cells after 48 hrs by XTT assay
|
[PMID: 28094938] |
| K562 | IC50 |
800 μM
Compound: AICAR
|
Cytotoxicity in drug sensitive human K562 cells assessed as reduction cell viability incubated for 48 hrs by XTT assay
Cytotoxicity in drug sensitive human K562 cells assessed as reduction cell viability incubated for 48 hrs by XTT assay
|
[PMID: 29655981] |
| K-562R | IC50 |
800 μM
Compound: AICAR
|
Cytotoxicity in imatinib-resistant human K562R cells assessed as reduction cell viability incubated for 48 hrs by XTT assay
Cytotoxicity in imatinib-resistant human K562R cells assessed as reduction cell viability incubated for 48 hrs by XTT assay
|
[PMID: 29655981] |
| L1210 | IC50 |
≥250 μM
Compound: 13, AICA
|
Cytostatic activity against mouse L1210 cells assessed as growth inhibition after 2 days by particle counting analysis
Cytostatic activity against mouse L1210 cells assessed as growth inhibition after 2 days by particle counting analysis
|
[PMID: 24177359] |
| MDA-MB-231 | IC50 |
1 mM
Compound: AICAR
|
Anticancer activity against human MDA-MB-231 cells after 48 hrs by XTT assay
Anticancer activity against human MDA-MB-231 cells after 48 hrs by XTT assay
|
[PMID: 28325600] |
| MDA-MB-453 | IC50 |
1700 μM
Compound: AICAR
|
Antiproliferation activity against human MDA-MB-453 cells assessed as reduction in cell viability measured after 4 days by Celltiter-glo luminescent cell viability assay
Antiproliferation activity against human MDA-MB-453 cells assessed as reduction in cell viability measured after 4 days by Celltiter-glo luminescent cell viability assay
|
[PMID: 32007387] |
| RCC4 | IC50 |
250 μM
Compound: AICAR
|
Anticancer activity against human RCC4 cells after 48 hrs by XTT assay
Anticancer activity against human RCC4 cells after 48 hrs by XTT assay
|
[PMID: 28325600] |
| SK-BR-3 | IC50 |
180 μM
Compound: AICAR
|
Antiproliferation activity against human SK-BR-3 cells assessed as reduction in cell viability measured after 4 days by Celltiter-glo luminescent cell viability assay
Antiproliferation activity against human SK-BR-3 cells assessed as reduction in cell viability measured after 4 days by Celltiter-glo luminescent cell viability assay
|
[PMID: 32007387] |
HepG2 cells are treated with various concentrations of AICAR (0.1-1.0 mM) for 12, 24, and 48 h, respectively. The expression level of IR-β significantly decreases with 0.25, 0.5, and 1.0 mM of AICAR at 48 h to 50%, 53%, and 46% of the control, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
-
CAS No. 2627-69-2
-
Appearance Solid
-
Masse moléculaire 258.23
-
Formule C9H14N4O5
-
Color White to light yellow
-
SMILES
OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(N)=C(N=C2)C(N)=O)O1
-
Synonyms
Acadesine; AICA Riboside
-
Structure Classification
-
Initial Source
-
Livraison
Room temperature in continental US; may vary elsewhere.
-
Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (216)
-
Journal Impact Factor
-
Most Recent
-
Signal Transduct Target Ther
A two-strata energy flux system driven by a stress hormone prioritizes cardiac energetics. [Abstract]2025 Sep 26;10(1):315. PMID: 40998786 -
Signal Transduct Target Ther
Phosphorylation of PHF2 by AMPK releases the repressive H3K9me2 and inhibits cancer metastasis. [Abstract]2023 Mar 6;8(1):95. PMID: 36872368 -
Signal Transduct Target Ther
Nuclear genome-derived circular RNA circPUM1 localizes in mitochondria and regulates oxidative phosphorylation in esophageal squamous cell carcinoma. [Abstract]2022 Feb 14;7(1):40. PMID: 35153295 -
Nature
2025 Feb;638(8052):1112-1121. PMID: 39814884 -
Cancer Cell
SETD2 Restricts Prostate Cancer Metastasis by Integrating EZH2 and AMPK Signaling Pathways. [Abstract]2020 Sep 14;38(3):350-365.e7. PMID: 32619406 -
Cell Metab
Dietary timing enhances exercise by modulating fat-muscle crosstalk via adipocyte AMPKα2 signaling. [Abstract]2025 Jun 3;37(6):1364-1380.e6. PMID: 40088888 -
Cell Metab
2019 Jul 2;30(1):157-173.e7 PMID: 31155494 -
Cancer Res
MTHFD2 Enhances cMYC O-GlcNAcylation to Promote Sunitinib Resistance in Renal Cell Carcinoma. [Abstract]2025 Mar 14;85(6):1113-1129. PMID: 39804969 -
Cancer Res
Fructose-induced mTORC1 Activation Promotes Pancreatic Cancer Progression through Inhibition of Autophagy. [Abstract]2023 Dec 15;83(24):4063-4079. PMID: 37738413 -
ACS Nano
Reprogramming of Myeloid Responses to Volumetric Muscle Loss via Engineered Protein Nanoparticles. [Abstract]2025 Aug 26;19(33):30441-30454. PMID: 40788769 -
Cell Death Differ
NDUFS3 promotes proliferation via glucose metabolism reprogramming inducing AMPK phosphorylating PRPS1 to increase the purine nucleotide synthesis in melanoma. [Abstract]2025 May 22. PMID: 40404919 -
Cell Death Differ
Branched-chain amino acid aminotransferase 2 regulates ferroptotic cell death in cancer cells. [Abstract]2021 Apr;28(4):1222-1236. PMID: 33097833 -
Adv Sci (Weinh)
NADH-Reductive Stress Induced by Dihydrolipoamide Dehydrogenase Activation Contributes to Cuproptosis. [Abstract]2025 Dec 5:e20444. PMID: 41346305 -
Adv Sci (Weinh)
Flipping the Switch: MeCP2-Mediated Lactylation Rewires Microglial Metabolism and Inflammation via the HK2/mTOR Axis in Poststroke Neuroinflammation. [Abstract]2025 Dec 22:e13400. PMID: 41429663 -
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J Exp Clin Cancer Res
2021 Jun 23;40(1):206. PMID: 34162423 -
J Nanobiotechnology
Liposome-encapsulated AICAR hydrogel regulates macrophage metabolic reprogramming via SIK1 activation to alleviate osteoarthritis. [Abstract]2025 Jul 5;23(1):486. PMID: 40616103 -
Redox Biol
Apolipoprotein C3 exacerbates vascular calcification by promoting ferroptosis via the TLR2/AMPK pathway in vascular smooth muscle cells. [Abstract]2026 Apr:91:104088. PMID: 41734579 -
Redox Biol
Pharmaceutical targeting of succinate dehydrogenase in fibroblasts controls bleomycin-induced lung fibrosis. [Abstract]2021 Oct:46:102082. PMID: 34343908 -
Gut Microbes
High-fat diet-induced L-saccharopine accumulation inhibits estradiol synthesis and damages oocyte quality by disturbing mitochondrial homeostasis. [Abstract]2024 Jan-Dec;16(1):2412381. PMID: 39410876 -
Pharmacol Res
PXR triggers YAP-TEAD binding and Sirt2-driven YAP deacetylation and polyubiquitination to promote liver enlargement and regeneration in mice. [Abstract]2023 Feb:188:106666. PMID: 36657504 -
Pharmacol Res
Lycorine improves peripheral nerve function by promoting Schwann cell autophagy via AMPK pathway activation and MMP9 downregulation in diabetic peripheral neuropathy. [Abstract]2022 Jan:175:105985. PMID: 34863821 -
Pharmacol Res
Hyperoside relieves particulate matter-induced lung injury by inhibiting AMPK/mTOR-mediated autophagy deregulation. [Abstract]2021 May:167:105561. PMID: 33737241 -
Cell Mol Biol Lett
SESN2 prevents the slow-to-fast myofiber shift in denervated atrophy via AMPK/PGC-1α pathway. [Abstract]2022 Aug 9;27(1):66. PMID: 35945510 -
Cancer Lett
SLC5A11 mediates metformin-induced PD-L1 suppression to enhance cancer immunotherapy through AMPK-IRF1 signaling. [Abstract]2026 Apr 28:644:218311. PMID: 41690450 -
Cancer Lett
Fusobacterium nucleatum enhances cholesterol biosynthesis in colorectal cancer via miR-130a-3p-mediated AMPK inhibition, a process counteracted by butyrate. [Abstract]2025 May 23:217810. PMID: 40414519 -
Int J Biol Sci
Chronic Stress-induced Serotonin Impairs Intestinal Epithelial Cell Mitochondrial Biogenesis via the AMPK-PGC-1α Axis. [Abstract]2024 Aug 19;20(11):4476-4495. PMID: 39247815 -
Int J Biol Sci
URI1 suppresses irradiation-induced reactive oxygen species (ROS) by activating autophagy in hepatocellular carcinoma cells. [Abstract]2021 Jul 22;17(12):3091-3103. PMID: 34421352 -
Cell Death Dis
Irisin regulates oxidative stress and mitochondrial dysfunction through the UCP2-AMPK pathway in prion diseases. [Abstract]2025 Feb 3;16(1):66. PMID: 39900919 -
Cell Death Dis
LncRNA PVT1 induces mitochondrial dysfunction of podocytes via TRIM56 in diabetic kidney disease. [Abstract]2024 Sep 30;15(9):697. PMID: 39349450 -
Cell Death Dis
High glucose suppresses autophagy through the AMPK pathway while it induces autophagy via oxidative stress in chondrocytes. [Abstract]2021 May 18;12(6):506. PMID: 34006821 -
Cell Death Dis
Iron overload promotes mitochondrial fragmentation in mesenchymal stromal cells from myelodysplastic syndrome patients through activation of the AMPK/MFF/Drp1 pathway. [Abstract]2018 May 1;9(5):515. PMID: 29725013
AICAR purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2018 May 1;9(5):515. [Abstract]
Protein expression levels of AMPKα, p-AMPKα-thr172, p-ACC-ser79, LC3, Beclin-1, ATG5 and ATG7 in WT MSCs or control CrispCas MSCs, or AMPKα DKO MSCs before and after treatment with FAC or AICAR.
-
Proc Natl Acad Sci U S A
2024 Oct 29;121(44):e2321305121. PMID: 39436655 -
Cell Commun Signal
Inhibition of SGLT2 protects podocytes in diabetic kidney disease by rebalancing mitochondria-associated endoplasmic reticulum membranes. [Abstract]2024 Nov 7;22(1):534. PMID: 39511548 -
Cell Commun Signal
Promoting AMPK/SR-A1-mediated clearance of HMGB1 attenuates chemotherapy-induced peripheral neuropathy. [Abstract]2023 May 4;21(1):99. PMID: 37143083 -
Int J Biol Macromol
A novel long non-coding RNA, Lnc-GAS7, modulates myosin heavy chain isoform switching via AMPK signaling in ovine muscle. [Abstract]2025 Oct 12;330(Pt 4):148291. PMID: 41086893 -
Int J Biol Macromol
NDRG2 regulates milkfatbiosynthesis by targeting the AMPK/SREBP1 axis in bovine mammary epithelial cells. [Abstract]2025 Sep;321(Pt 4):146448. PMID: 40749917 -
Int J Biol Macromol
Downregulation of VEGFA accelerates AGEs-mediated nucleus pulposus degeneration through inhibiting protective mitophagy in high glucose environments. [Abstract]2024 Feb 5;262(Pt 1):129950. PMID: 38320636 -
Int J Biol Macromol
NPFR regulates the synthesis and metabolism of lipids and glycogen via AMPK: Novel targets for efficient corn borer management. [Abstract]2023 Aug 30:247:125816. PMID: 37451386 -
Phytomedicine
Swiprosin-1 participates in the berberine-regulated AMPK/MLCK pathway to attenuate colitis-induced tight junction damage. [Abstract]2024 Dec:135:156111. PMID: 39369569 -
Phytomedicine
Insights into the mechanism of action of pterostilbene against influenza A virus-induced acute lung injury. [Abstract]2024 Jul:129:155534. PMID: 38583346 -
Phytomedicine
Arctium lappa L. roots ameliorates cerebral ischemia through inhibiting neuronal apoptosis and suppressing AMPK/mTOR-mediated autophagy. [Abstract]2021 May:85:153526. PMID: 33691269 -
NPJ Parkinsons Dis
Disruption of axonal transport in Parkinson's disease: the role of pathological α-Syn and AMPK/p38 MAPK signaling. [Abstract]2025 May 6;11(1):114. PMID: 40328804 -
Free Radic Biol Med
Methionine sulfoxide reductase A deficiency aggravated ferroptosis in LPS-induced acute kidney injury by inhibiting the AMPK/NRF2 axis and activating the CaMKII/HIF-1α pathway. [Abstract]2025 Apr 25:S0891-5849(25)00250-3. PMID: 40288699 -
Free Radic Biol Med
Empagliflozin attenuates the renal tubular ferroptosis in diabetic kidney disease through AMPK/NRF2 pathway. [Abstract]2023 Feb 1:195:89-102. PMID: 36581059 -
Regen Biomater
Fabrication of a 3D bioprinting model for posterior capsule opacification using GelMA and PLMA hydrogel-coated resin. [Abstract]2024 Mar 1:11:rbae020. PMID: 38529352 -
Food Res Int
Quercetin alleviates ethanol-induced hepatocyte senescence by interacting with glycinamide ribonucleotide transformylase. [Abstract]2025 Dec;221(Pt 3):117392. PMID: 41215006 -
Clin Sci
Glutamine alleviates immunosuppression in polymicrobial sepsis by augmenting bacterial phagocytosis through sustaining the GFAT-DRP1 dependent mitochondrial calcium dynamics. [Abstract]2025 Oct 22;139(20):1163-1185. PMID: 41082631 -
Br J Pharmacol
Contributions of synaptic energetic dysfunction by microtubule dynamics and microtubule-based mitochondrial transport disorder to morphine tolerance. [Abstract]2025 May 13. PMID: 40361281 -
Alzheimers Res Ther
Photobiomodulation mitigates blood-brain barrier disruption in APP/PS1 mouse model of Alzheimer's disease by activating the AMPK pathway. [Abstract]2025 Jun 23;17(1):141. PMID: 40551167 -
Biomed Pharmacother
Canagliflozin mitigates carfilzomib-induced endothelial apoptosis via an AMPK-dependent pathway. [Abstract]2023 Aug:164:114907. PMID: 37247463 -
Europace
Promoting differentiation of human-induced pluripotent stem cells into sinoatrial node-like cells through programmed regulation of AMPK signalling pathway. [Abstract]2025 Oct 31;27(11):euaf288. PMID: 41206581 -
Stem Cell Res Ther
2025 Apr 24;16(1):206. PMID: 40275329 -
Environ Pollut
Maltol attenuates polystyrene nanoplastic-induced enterotoxicity by promoting AMPK/mTOR/TFEB-mediated autophagy and modulating gut microbiota. [Abstract]2023 Feb 7;322:121202. PMID: 36736819 -
Oncogene
Negative regulation of AMPKα1 by PIM2 promotes aerobic glycolysis and tumorigenesis in endometrial cancer. [Abstract]2019 Sep;38(38):6537-6549. PMID: 31358902 -
Cell Chem Biol
Genetically encoded manipulation of ATP/ADP ratio in human cells uncovers proteomic and physiological signatures of energy stress. [Abstract]2026 May 21;33(5):683-698.e7. PMID: 41980778 -
Curr Res Food Sci
Dietary kaempferol exerts anti-obesity effects by inducing the browing of white adipocytes via the AMPK/SIRT1/PGC-1α signaling pathway. [Abstract]2024 Mar 28:8:100728. PMID: 38577419 -
Cell Death Discov
2024 Jan 17;10(1):34. PMID: 38233385 -
Cell Rep
circPRKAA1 activates a Ku80/Ku70/SREBP-1 axis driving de novo fatty acid synthesis in cancer cells. [Abstract]2022 Nov 22;41(8):111707. PMID: 36417875 -
Cell Rep
2022 Aug 16;40(7):111204. PMID: 35977508 -
Cell Rep
DOT1L O-GlcNAcylation promotes its protein stability and MLL-fusion leukemia cell proliferation. [Abstract]2021 Sep 21;36(12):109739. PMID: 34551297 -
Arch Toxicol
Upregulation of phosphoinositide 3-kinase prevents sunitinib-induced cardiotoxicity in vitro and in vivo. [Abstract]2019 Jun;93(6):1697-1712. PMID: 31025080 -
Br J Cancer
An intrinsic purine metabolite AICAR blocks lung tumour growth by targeting oncoprotein mucin 1. [Abstract]2023 May;128(9):1647-1664. PMID: 36810913 -
Clin Transl Med
MD2 deficiency prevents high-fat diet-induced AMPK suppression and lipid accumulation through regulating TBK1 in non-alcoholic fatty liver disease. [Abstract]2022 Mar;12(3):e777. PMID: 35343085 -
Clin Transl Med
Low-glucose-sensitive TRPC6 dysfunction drives hypoglycemia-induced cognitive impairment in diabetes. [Abstract]2020 Oct;10(6):e205. PMID: 33135341 -
Neural Regen Res
Chitosan alleviates symptoms of Parkinson's disease by reducing acetate levels, which decreases inflammation and promotes repair of the intestinal barrier and blood-brain barrier. [Abstract]2026 Jan 1;21(1):377-391. PMID: 38934394 -
J Anim Sci Biotechnol
Vitamin A-activated PPARγ signaling enhances intramuscular fat accumulation by overriding AMPK-mediated inhibition in late-fattening beef cattle. [Abstract]2026 Feb 12;17(1):29. PMID: 41673695 -
J Anim Sci Biotechnol
Single-base editing in IGF2 improves meat production and intramuscular fat deposition in Liang Guang Small Spotted pigs. [Abstract]2023 Nov 2;14(1):141. PMID: 37919760 -
Phytother Res
Aqueous extract of clove inhibits tumor growth by inducing autophagy through AMPK/ULK pathway. [Abstract]2019 Jul;33(7):1794-1804. PMID: 30993793 -
J Agric Food Chem
Natural Dietary Flavonoid Apigenin Mitigates Ulcerative Colitis via Modulating the AMPK/NF-κB/NLRP3 Signaling Axis. [Abstract]2025 Dec 22. PMID: 41428381 -
J Agric Food Chem
Exposure to Succinate Leads to Steatosis in Non-Obese Non-Alcoholic Fatty Liver Disease by Inhibiting AMPK/PPARα/FGF21-Dependent Fatty Acid Oxidation. [Abstract]2024 Sep 25;72(38):21052-21064. PMID: 39268842 -
J Agric Food Chem
Platycodin D Ameliorates Type 2 Diabetes-Induced Myocardial Injury by Activating the AMPK Signaling Pathway. [Abstract]2024 May 8;72(18):10339-10354. PMID: 38682702 -
J Agric Food Chem
CIDEA Regulates De Novo Fatty Acid Synthesis in Bovine Mammary Epithelial Cells by Targeting the AMPK/PPARγ Axis and Regulating SREBP1. [Abstract]2022 Sep 14;70(36):11324-11335. PMID: 36040348 -
J Agric Food Chem
Regulation on Citrate Influx and Metabolism through Inhibiting SLC13A5 and ACLY: A Novel Mechanism Mediating the Therapeutic Effects of Curcumin on NAFLD. [Abstract]2021 Aug 11;69(31):8714-8725. PMID: 34323067 -
J Agric Food Chem
Protocatechuic Acid-Ameliorated Endothelial Oxidative Stress through Regulating Acetylation Level via CD36/AMPK Pathway. [Abstract]2019 Jun 26;67(25):7060-7072. PMID: 31240928 -
Ecotoxicol Environ Saf
Aluminum-maltol induced oxidative stress and reduced AMPK activity via BCK-related energy supply failure in C6 cell. [Abstract]2024 Jan 15:270:115831. PMID: 38101974 -
Antioxid Redox Signal
Mitofusin2 ameliorated ER stress and mitochondrial ROS through maintaining mitochondria-associated ER membrane integrity in cisplatin-induced acute kidney injury. [Abstract]2024 Jan;40(1-3):16-39. PMID: 37053105
AICAR purchased from MedChemExpress. Usage Cited in: Antioxid Redox Signal. 2024 Jan;40(1-3):16-39. [Abstract]
AICAR (0.5 mM; 12 h) significantly increases the expression of Mfn2 in BUMPT Cells.
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JCI Insight
Recurrent moderate hypoglycemia accelerates the progression of Alzheimer's disease through impairment of the TRPC6/GLUT3 pathway. [Abstract]2022 Mar 8;7(5):e154595. PMID: 35077394 -
Cell Biol Toxicol
UHRF1 promotes calcium oxalate-induced renal fibrosis by renal lipid deposition via bridging AMPK dephosphorylation. [Abstract]2025 Feb 3;41(1):39. PMID: 39899077 -
Front Immunol
LPS Stimulation Induces Small Heterodimer Partner Expression Through the AMPK-NRF2 Pathway in Large Yellow Croaker (Larimichthys crocea). [Abstract]2021 Nov 8:12:753681. PMID: 34819934 -
Int J Mol Med
Jinlida granules alleviate podocyte apoptosis and mitochondrial dysfunction via the AMPK/PGC‑1α pathway in diabetic nephropathy. [Abstract]2025 Feb;55(2):26. PMID: 39670303 -
Int J Mol Med
Free fatty acids induce coronary microvascular dysfunction via inhibition of the AMPK/KLF2/eNOS signaling pathway. [Abstract]2023 Apr;51(4):34. PMID: 36928181 -
Int J Mol Med
High glucose suppresses the viability and proliferation of HTR‑8/SVneo cells through regulation of the miR‑137/PRKAA1/IL‑6 axis. [Abstract]2018 Aug;42(2):799-810. PMID: 29786111 -
Int J Mol Med
AMP-activated protein kinase stimulates osteoblast differentiation and mineralization through autophagy induction. [Abstract]2018 May;41(5):2535-2544. PMID: 29484369
AICAR purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2018 May;41(5):2535-2544. [Abstract]
Cells are treated with AICAR and AICAR (A++, 10 μM) + Compound C (C++, 1 μM) for 24 h, after which western blot analysis is performed.
AICAR purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2018 May;41(5):2535-2544. [Abstract]
MC3T3-E1 cells are treated with AICAR (10 μM) in the presence or absence of 3-MA (5 mM) or CQ (10 μM) for 24 h, after which western blot analysis is conducted.
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Chin Med
A novel Chinese medicine formula against hyperuricemia-induced kidney inflammation involving pyroptosis inhibition via modulating AMPK-TLR4-NLRP3 pathway. [Abstract]2026 Mar 1;21(1):74. PMID: 41764514 -
Chin Med
Buyang Huanwu Decoction improves energy metabolism disorders after cerebral ischemia-reperfusion by regulating the SIRT1/AMPK signaling pathway to promote glycolysis and the tricarboxylic acid cycle. [Abstract]2025 Jul 7;20(1):108. PMID: 40624684 -
Biochem Pharmacol
Glimepiride overcomes acquired resistance to EGFR TKIs in lung cancer via the AMPK/ERK/MMP7 signaling pathway. [Abstract]2025 Jul 12:241:117162. PMID: 40659128 -
Am J Chin Med
Using Network Pharmacology for Systematic Understanding of Geniposide in Ameliorating Inflammatory Responses in Colitis Through Suppression of NLRP3 Inflammasome in Macrophage by AMPK/Sirt1 Dependent Signaling. [Abstract]2020;48(7):1693-1713. PMID: 33202149 -
Food Funct
Kaempferol alleviates right ventricular hypertrophy in high-altitude pulmonary hypertension rats by modulating the AMPK/ACC/CPT1B axis and the AMPK-mediated LDHA/PDHA1 pathway. [Abstract]2026 Apr 14;17(7):3172-3185. PMID: 41804727 -
J Ethnopharmacol
Huaiqihuang granule attenuate renal injury in IgA vasculitis nephritis by activating AMPK/mTOR-mediated autophagy. [Abstract]2026 Apr 6:360:121151. PMID: 41490554 -
J Ethnopharmacol
Corydalis saxicola Bunting total alkaloids improve NAFLD by suppressing de novo lipogenesis through the AMPK-SREBP1 axis. [Abstract]2024 Jan 30;319(Pt 1):117162. PMID: 37690477 -
J Ethnopharmacol
Mailuoning oral liquid attenuates convalescent cerebral ischemia by inhibiting AMPK/mTOR-associated apoptosis and promoting CREB/BDNF-mediated neuroprotection. [Abstract]2023 Dec 5:317:116731. PMID: 37277084 -
Food Funct
Activation of AMPK/Sirt3 pathway by phloretin reduces mitochondrial ROS in vascular endothelium by increasing the activity of MnSOD via deacetylation. [Abstract]2020 Apr 1;11(4):3073-3083. PMID: 32195489 -
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Cells
The Degradation of TMEM166 by Autophagy Promotes AMPK Activation to Protect SH-SY5Y Cells Exposed to MPP. [Abstract]2022 Aug 30;11(17):2706. PMID: 36078115 -
Commun Biol
The fungistatic mechanism of benzaldehyde against the nematophagous fungus Arthrobotrys oligospora suggests a method for manipulating soil fungistasis. [Abstract]2026 Mar 10;9(1):566. PMID: 41807727 -
Commun Biol
Human placenta mesenchymal stromal cells alleviate intestinal inflammation and repair intestinal barrier function by activating AMPK-FXR pathway. [Abstract]2025 May 30;8(1):830. PMID: 40447793 -
Nutrients
High Fat Activates O-GlcNAcylation and Affects AMPK/ACC Pathway to Regulate Lipid Metabolism. [Abstract]2021 May 21;13(6):1740. PMID: 34063748 -
Nutrients
The Extract of Arctium lappa L. Fruit (Arctii Fructus) Improves Cancer-Induced Cachexia by Inhibiting Weight Loss of Skeletal Muscle and Adipose Tissue. [Abstract]2020 Oct 19;12(10):3195. PMID: 33086629 -
J Affect Disord
Ketamine ameliorates postpartum depression-like behaviors in rats exposed to sevoflurane during pregnancy through the AMPK/SIRT1/NLRP3 pathway. [Abstract]2026 Jun 15:403:121439. PMID: 41698615 -
Int J Mol Sci
Vitisin A Outperforms Cyanidin-3-O-Glucoside in Triglyceride Reduction by Modulating Hepatic Lipogenesis and Fatty Acid β-Oxidation. [Abstract]2025 Feb 11;26(4):1521. PMID: 40003987 -
Front Pharmacol
Metformin attenuates diabetic osteoporosis by suppressing ferroptosis via the AMPK/Nrf2 pathway. [Abstract]2025 Mar 26:16:1527316. PMID: 40206070 -
Front Pharmacol
Berberine stimulates lysosomal AMPK independent of PEN2 and maintains cellular AMPK activity through inhibiting the dephosphorylation regulator UHRF1. [Abstract]2023 Apr 18:14:1148611. PMID: 37144221 -
Front Pharmacol
Metformin Ameliorates Chronic Colitis-Related Intestinal Fibrosis via Inhibiting TGF-β1/Smad3 Signaling. [Abstract]2022 May 13;13:887497. PMID: 35645830 -
Front Pharmacol
Geniposide Combined With Notoginsenoside R1 Attenuates Inflammation and Apoptosis in Atherosclerosis via the AMPK/mTOR/Nrf2 Signaling Pathway. [Abstract]2021 Jul 7:12:687394. PMID: 34305600 -
Front Pharmacol
Eye Drops of Metformin Prevents Fibrosis After Glaucoma Filtration Surgery in Rats via Activating AMPK/Nrf2 Signaling Pathway. [Abstract]2020 Jul 31;11:1038. PMID: 32903813 -
Front Pharmacol
Activating AMPK to Restore Tight Junction Assembly in Intestinal Epithelium and to Attenuate Experimental Colitis by Metformin. [Abstract]2018 Jul 16:9:761. PMID: 30061832
AICAR purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2018 Jul 16:9:761. [Abstract]
Epithelial cells from colon tissue are extracted from mice in each group (n=6-8 per group) and protein level of p-AMPK (Thr172) and AMPKα1/2 are measured by western blot.
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Front Pharmacol
Astragaloside IV Inhibits Triglyceride Accumulation in Insulin-Resistant HepG2 Cells via AMPK-Induced SREBP-1c Phosphorylation. [Abstract]2018 Apr 16:9:345. PMID: 29713279
AICAR purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2018 Apr 16:9:345. [Abstract]
AMP-activated protein kinase (AMPK) activation by AST blunts dysfunction of lipid metabolism and resistance in HepG2 cells. Representative bands of SREBP-1c nuclear protein and cytoplasmic proteins are visualized in the immunoblotting assay.
AICAR purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2018 Apr 16:9:345. [Abstract]
AMP-activated protein kinase activation by AST inhibits the expression of sterol regulatory element binding protein-1c (SREBP-1c) and SREBP-1c related genes. AST promotes AMPKa1 mRNA expression, AMPKa2 mRNA expression, and suppresses the expression of SREBP-1c, and its downstream genes FAS, ACC1, and also AMPK downstream gene SCD1.
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Int Immunopharmacol
Baicalein limits subchondral bone lesions via AMPKα/BECN1 activation in osteoarthritis osteoblast. [Abstract]2026 Apr 15:175:116446. PMID: 41791307 -
mBio
The swine acute diarrhea syndrome coronavirus spike protein promotes syncytial formation via upregulation of cellular cholesterol synthesis. [Abstract]2025 Aug 13;16(8):e0097625. PMID: 40586601 -
Eur J Pharmacol
Resveratrol attenuates cyclosporin A-induced upregulation of the thromboxane A2 receptor and hypertension via the AMPK/SIRT1 and MAPK/NF-κB pathways in the rat mesenteric artery. [Abstract]2024 Jun 5:972:176543. PMID: 38582274 -
Int Immunopharmacol
Acadesine alleviates acute pancreatitis-related lung injury by mediating the barrier protective function of pulmonary microvascular endothelial cells. [Abstract]2022 Oct:111:109165. PMID: 35987144 -
Int Immunopharmacol
The role of AMPK-Sirt1-autophagy pathway in the intestinal protection process by propofol against regional ischemia/reperfusion injury in rats. [Abstract]2022 Oct:111:109114. PMID: 35933747 -
Int Immunopharmacol
SIRT3-AMPK signaling pathway as a protective target in endothelial dysfunction of early sepsis. [Abstract]2022 May:106:108600. PMID: 35217431 -
Neuropharmacology
SARM1 deficiency induced depressive-like behavior via AMPKα/p-eEF2 axis to synapse dysfunction. [Abstract]2024 Nov 1:110206. PMID: 39489288 -
Front Microbiol
Porcine Circovirus Type 2 ORF5 Protein Induces Autophagy to Promote Viral Replication via the PERK-eIF2α-ATF4 and mTOR-ERK1/2-AMPK Signaling Pathways in PK-15 Cells. [Abstract]2020 Feb 28;11:320. PMID: 32184774 -
FASEB J
Regulator of Calcineurin 1 Aggravates Tubular Epithelial Cell Injury in Diabetic Mice Through Promoting Liver Kinase B1 Ubiquitination. [Abstract]2026 Mar 31;40(6):e71712. PMID: 41854627 -
Poult Sci
2026 Feb 1;105(4):106573. PMID: 41650643 -
Exp Neurol
Exercise-induced Sesn2 mediates autophagic flux to alleviate neural damage after ischemic stroke in mice. [Abstract]2025 Feb 2:386:115174. PMID: 39904418 -
Biochim Biophys Acta Mol Basis Dis
IFN-γ-responsiveness of lymphatic endothelial cells inhibits melanoma lymphatic dissemination via AMPK-mediated metabolic control. [Abstract]2024 Jun 25:167314. PMID: 38936516 -
Mol Nutr Food Res
Hyperglycemia Inhibits Hepatic SHBG Synthesis Through the NGBR-AMPK-HNF4 Pathway in Rats with Polycystic Ovary Syndrome Induced by Letrozole in Combination with a High-Fat Diet. [Abstract]2024 Jun 11:e2300915. PMID: 38862276 -
J Cell Mol Med
NR3C2 inhibits the proliferation of colorectal cancer via regulating glucose metabolism and phosphorylating AMPK. [Abstract]2023 Apr;27(8):1069-1082. PMID: 36950803 -
Biochim Biophys Acta Mol Basis Dis
Myeloid differential protein-2 inhibition improves diabetic cardiomyopathy via p38MAPK inhibition and AMPK pathway activation. [Abstract]2022 May 1;1868(5):166369. PMID: 35176461 -
J Cell Mol Med
Interfering with mitochondrial dynamics sensitizes glioblastoma multiforme to temozolomide chemotherapy. [Abstract]2022 Feb;26(3):893-912. PMID: 34964241 -
FASEB J
Rspo1/Rspo3-LGR4 signaling inhibits hepatic cholesterol synthesis through the AMPKα-SREBP2 pathway. [Abstract]2020 Nov;34(11):14946-14959. PMID: 32926477 -
J Cell Mol Med
TIPE1-mediated autophagy suppression promotes nasopharyngeal carcinoma cell proliferation via the AMPK/mTOR signalling pathway. [Abstract]2020 Aug;24(16):9135-9144. PMID: 32588529 -
Biochim Biophys Acta Mol Basis Dis
Alpha-lipoic acid regulates the autophagy of vascular smooth muscle cells in diabetes by elevating hydrogen sulfide level. [Abstract]2018 Nov;1864(11):3723-3738. PMID: 30251691
AICAR purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta Mol Basis Dis. 2018 Nov;1864(11):3723-3738. [Abstract]
The effect of AICAR on the expression of AMPK, p-AMPK, mTOR, p-mTOR, and autophagy-related protein by Western blot analysis.
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iScience
Roquin-1 resolves sepsis-associated acute liver injury by regulating inflammatory profiles via miRNA cargo in extracellular vesicles. [Abstract]2023 Jul 12;26(8):107295. PMID: 37554446 -
Biomed Environ Sci
2019 Dec;32(12):944-948. PMID: 31918802 -
Antiviral Res
Metformin inhibits EV-A71 and CVA16 infections by regulating TRIB3-SCARB2 axis and activating AMPK. [Abstract]2025 Jan 16:235:106081. PMID: 39826812 -
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Antiviral Res
Identification of desoxyrhapontigenin as a novel antiviral agent against congenital Zika virus infection. [Abstract]2023 Mar:211:105542. PMID: 36646387 -
J Integr Med
Qili Qiangxin, a compound herbal medicine formula, alleviates hypoxia-reoxygenation-induced apoptotic and autophagic cell death via suppression of ROS/AMPK/mTOR pathway in vitro. [Abstract]2022 Jul;20(4):365-375. PMID: 35534381 -
J Cell Physiol
Hyaluronidase2 (Hyal2) modulates low shear stress-induced glycocalyx impairment via the LKB1/AMPK/NADPH oxidase-dependent pathway. [Abstract]2018 Dec;233(12):9701-9715. PMID: 30078213
AICAR purchased from MedChemExpress. Usage Cited in: J Cell Physiol. 2018 Dec;233(12):9701-9715. [Abstract]
HUVECs are pretreated with Compound C (5, 10, or 20 μM) for 18 hr or AICAR (1, 2, or 4 mM) for 1 hr, and then exposed to LSS for 30 min.
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Sci Rep
AMPK agonist AICAR ameliorates maternal hepatic lipid metabolism disorder, inflammation, and fibrosis caused by PM2.5 exposure during pregnancy. [Abstract]2025 Mar 13;15(1):8689. PMID: 40082541 -
Aquac Nutr
Branched-Chain Amino Acids Target miR-203a/fosb Axis to Promote Skeletal Muscle Growth in Common Carp (Cyprinus carpio). [Abstract]2025 Feb 26:2025:9406490. PMID: 40046093 -
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J Biol Chem
ACADM inhibits AMPK activation to modulate PEDV-induced lipophagy and β-oxidation for impairing viral replication. [Abstract]2024 Jul 11:107549. PMID: 39002673 -
J Biol Chem
Glucose-6-phosphate dehydrogenase exerts anti-stress effects independently of its enzymatic activity. [Abstract]2022 Dec;298(12):102587. PMID: 36243112 -
Biomedicines
Microtubule Affinity-Regulating Kinase 4 Promotes Oxidative Stress and Mitochondrial Dysfunction by Activating NF-κB and Inhibiting AMPK Pathways in Porcine Placental Trophoblasts. [Abstract]2022 Jan 13;10(1):165. PMID: 35052845 -
Neurochem Res
miR-210 Regulates Autophagy Through the AMPK/mTOR Signaling Pathway, Reduces Neuronal Cell Death and Inflammatory Responses, and Enhances Functional Recovery Following Cerebral Hemorrhage in Mice. [Abstract]2025 Jun 5;50(3):180. PMID: 40471451 -
J Virol
2024 Mar 19;98(3):e0175123. PMID: 38319105 -
Biochim Biophys Acta Mol Cell Res
Development of a TEAD-independent reporter system for specific and sensitive measurement of YAP/TAZ activity. [Abstract]2026 Mar;1873(3):120111. PMID: 41534572 -
Cell Signal
AMPK impairment caused by gelatin disturbs mitochondrial dynamics leading to enhanced phagocytosis of bacteria in PMA-stimulated U937 cells. [Abstract]2025 Nov:135:111985. PMID: 40653043 -
Cardiovasc Toxicol
Ciprofloxacin Accelerates Angiotensin-II-Induced Vascular Smooth Muscle Cells Senescence Through Modulating AMPK/ROS pathway in Aortic Aneurysm and Dissection. [Abstract]2024 Sep;24(9):889-903. PMID: 39138741 -
Cell Signal
AMPK-mediated autophagy pathway activation promotes ΔFosB degradation to improve levodopa-induced dyskinesia. [Abstract]2024 Jun:118:111125. PMID: 38432574 -
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Cell Signal
Autophagy activation attenuates the circadian clock oscillators in U2OS cells via the ATG5 pathway. [Abstract]2023 Jan:101:110502. PMID: 36280090 -
Biochim Biophys Acta Mol Cell Res
DDIT3/CHOP mediates the inhibitory effect of ER stress on chondrocyte differentiation by AMPKα-SIRT1 pathway. [Abstract]2022 Aug;1869(8):119265. PMID: 35381294 -
Enzyme Microb Technol
Expression and purification of asprosin in Pichia pastoris and investigation of its increase glucose uptake activity in skeletal muscle through activation of AMPK. [Abstract]2021 Mar:144:109737. PMID: 33541572 -
J Photochem Photobiol B
Photobiomodulation reduces hepatic lipogenesis and enhances insulin sensitivity through activation of CaMKKβ/AMPK signaling pathway. [Abstract]2020 Dec;213:112075. PMID: 33152638 -
Mol Cell Biochem
Advanced glycation end-products suppress autophagy by AMPK/mTOR signaling pathway to promote vascular calcification. [Abstract]2020 Aug;471(1-2):91-100. PMID: 32514882 -
Mol Cell Endocrinol
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Ginsenoside Rg1 inhibits apoptosis by increasing autophagy via the AMPK/mTOR signaling in serum deprivation macrophages. [Abstract]2018 Feb 1;50(2):144-155. PMID: 29324976
AICAR purchased from MedChemExpress. Usage Cited in: Acta Biochim Biophys Sin (Shanghai). 2018 Feb 1;50(2):144-155. [Abstract]
Raw264.7 macrophages with serum deprivation are treated with 50 μM Rg1 for 48 h in absence or presence of compound C (10 mM) or AICAR (250 μM). Western blots of the protein expressions of Atg5, Beclin1, LC3, and p62/SQSMT1.
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bioRxiv
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bioRxiv
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Solvant et solubilité
DMSO : 116.67 mg/mL (451.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 65 mg/mL (251.71 mM; Need ultrasonic and warming)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (19.36 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (19.36 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 110 mg/mL (425.98 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Protocole
HepG2 cells (5×105 cells) are plated in 6-well culture plate dishes and then are incubated in the serum-free media for 12 h before transfection. One microgram of plasmid is transfected with FuGENE6 Transfection Reagent. After 5 h of transfection, the culture media are removed and then media supplemented with or without AICAR (0.1-1.0 mM) are added to each well. The stimulation media are changed every 24 h[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Mice[2]
Fourteen-week-old lean (Lepob/+ or Lepob/+) and ob/ob (Lepob/Lepob) male mice are uesd. After the 14-day experimental treatment (24 h after AICAR injection, including a 12-h fast), the plantar flexor complex muscle is cleanly (tendon-to-tendon) excised from an anesthetized mouse. The muscle is quickly weighed and then processed for histology or frozen in liquid nitrogen and stored at -80°C. The anesthetized mice are killed by transection of the diaphragm and removal of the entire heart, after blood collection via needle puncture directly into the heart. AICAR or saline (control) is injected subcutaneously into the lateral distal portion of the back. AICAR is administered at 0.5 mg/g per day one time for 14 days. Saline (control) is injected in volumes identical to those used for AICAR treatment in a manner identical to that of AICAR treatment. Body weight is measured prior to death.
Rats[3]
Male 5-week-old ZDF rats are either subcutaneously injected with a single dose of AICAR (0.5 mg/g body wt) or underwent a single bout of treadmill running (60 min, speed of 25 m/min at a 5% incline). Untreated ZDF rats serve as controls (n=5 in each group). One hour after the subcutaneous AICAR injection or immediately after treadmill running, rats are killed by cervical dislocation. To avoid any effect of muscle spasm and hypoxia, red and white gastrocnemius muscles are removed within seconds and immediately freeze clamped for later determination of AMPK activity.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureté et documentation
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Fiche technique (286 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Nakamaru K, et al. AICAR, an activator of AMP-activated protein kinase, down-regulates the IR expression in HepG2 cells. Biochem Biophys Res Commun. 2005 Mar 11;328(2):449-54 [Content Brief]
[2]. Giri S, et al. 5-aminoimidazole-4-carboxamide-1-beta-4-ribofuranoside inhibits proinflammatory response in glial cells: a possible role of AMP-activated protein kinase. J Neurosci. 2004 Jan 14;24(2):479-87. [Content Brief]
[3]. Drake JC, et al. AICAR treatment for 14 days normalizes obesity-induced dysregulation of TORC1 signaling and translational capacity in fasted skeletal muscle. Am J Physiol Regul Integr Comp Physiol. 2010 Dec;299(6):R1546-54. [Content Brief]
[4]. Pold R, et al. Long-term AICAR administration and exercise prevents diabetes in ZDF rats.Diabetes. 2005 Apr;54(4):928-34. [Content Brief]
[5]. Ajaybabu V Pobbati, et al. A combat with the YAP/TAZ-TEAD oncoproteins for cancer therapy. Theranostics. 2020 Feb 18;10(8):3622-3635. [Content Brief]
[6]. Viana R, et al. Role of AMP-activated protein kinase in autophagy and proteasome function. Biochem Biophys Res Commun. 2008 May 9;369(3):964-8. [Content Brief]
Complete Work Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.8725 mL | 19.3626 mL | 38.7252 mL | 96.8129 mL |
| 5 mM | 0.7745 mL | 3.8725 mL | 7.7450 mL | 19.3626 mL | |
| 10 mM | 0.3873 mL | 1.9363 mL | 3.8725 mL | 9.6813 mL | |
| 15 mM | 0.2582 mL | 1.2908 mL | 2.5817 mL | 6.4542 mL | |
| 20 mM | 0.1936 mL | 0.9681 mL | 1.9363 mL | 4.8406 mL | |
| 25 mM | 0.1549 mL | 0.7745 mL | 1.5490 mL | 3.8725 mL | |
| 30 mM | 0.1291 mL | 0.6454 mL | 1.2908 mL | 3.2271 mL | |
| 40 mM | 0.0968 mL | 0.4841 mL | 0.9681 mL | 2.4203 mL | |
| 50 mM | 0.0775 mL | 0.3873 mL | 0.7745 mL | 1.9363 mL | |
| 60 mM | 0.0645 mL | 0.3227 mL | 0.6454 mL | 1.6135 mL | |
| 80 mM | 0.0484 mL | 0.2420 mL | 0.4841 mL | 1.2102 mL | |
| 100 mM | 0.0387 mL | 0.1936 mL | 0.3873 mL | 0.9681 mL |
* The product is commonly used at millimolar (mM) concentrations in cell experiments. We recommend weighing the desired amount and dissolving it in medium, followed by sterile filtration using a 0.22 μm filter before immediate use.
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