Cat. No.: HY-B1051

Flumethasone

(Synonyms: Flumetasone)

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

Flumethasone (Flumetasone) is an orally active, high selective and potent glucocorticoid receptor (GR) agonist. Flumethasone activates GR to inhibit nuclear factor kappa B (NF-κB)-mediated pro-inflammatory cytokine production (TNF-α, IL-1β) and promotes anti-inflammatory gene expression (IL-10), while also regulating metabolic enzyme activity (tyrosine aminotransferase induction). Flumethasone is promising for research of inflammatory diseases, cancer, and endocrine regulation.

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Pureté: 99.84%
CAS No.: 2135-17-3
Formule: C₂₂H₂₈F₂O₅
Masse moléculaire:410.45

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Flumethasone Chemical Structure

CAS No.: 2135-17-3

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Activité biologique
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

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In Vitro

Flumethasone (0-200 nM; 0-24 h) reduces Nrf2 protein levels through glucocorticoid receptor (GCR)-dependent proteasomal degradation in A549 and H460 cells^[1].
Flumethasone (100 nM; 4 h) enhances ubiquitination of Nrf2 in A549 and H460 cells, an effect blocked by GCR knockdown^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

A549 and H460 cells
Concentration:

50 nM, 100 nM, 200 nM
Incubation Time:

0-24 h
Result:

Decreased Nrf2 protein levels in a concentration-dependent manner, with significant reduction observed at 100 nM and 200 nM.

Immunofluorescence^[1]

Cell Line:

A549 and H460 cells
Concentration:

100 nM
Incubation Time:

4 h
Result:

Promoted Nrf2 ubiquitination, and this effect was abrogated in GCR-knockdown cells.

In Vivo

Flumethasone (10^-8 to 10^-6 moles/kg, i.p., a single dose) induces a 4- to 10-fold increase in liver tyrosine aminotransferase (TA) activity within 4–6 hours, with prolonged induction compared to hydrocortisone derivatives in adrenalectomized C57BL/6J mice or obese C57BL/6J-ob mutant mice ^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Adult male C57BL/6J mice and C57BL/6J-ob obese mutant mice^[2]
Dosage:

10^-8 to 10^-6 moles/kg
Administration:

i.p., a single dose
Result:

Induced a 10-fold increase in liver TA activity at 4 hr, significantly higher than hydrocortisone derivatives in adrenalectomized C57BL/6J mice.

Chemical Information

CAS No. 2135-17-3
Appearance Solid
Masse moléculaire 410.45
Formule C₂₂H₂₈F₂O₅
Color White to off-white
SMILES

C[C@@]12[C@](C(CO)=O)(O)[C@H](C)C[C@@]1([H])[C@]3([H])C[C@H](F)C4=CC(C=C[C@]4(C)[C@@]3(F)[C@@H](O)C2)=O
Synonyms

Flumetasone
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (1)

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Drug Test Anal

High-throughput liquid chromatography tandem mass spectrometry assay as initial testing procedure for analysis of total urinary fraction. [Abstract]

2021 Feb;13(2):283-298. PMID: 32852861

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (243.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4364 mL

12.1818 mL

24.3635 mL

5 mM

0.4873 mL

2.4364 mL

4.8727 mL

10 mM

0.2436 mL

1.2182 mL

2.4364 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (282 KB)
SDS (418 KB)
- English - EN (418 KB)
- Français - FR (418 KB)
- Deutsch - DE (418 KB)
- Norwegian - NO (418 KB)
- Español - ES (418 KB)
- Swedish - SV (418 KB)
- Italian - IT (418 KB)
- Korean - KR (418 KB)
- Portuguese - PT (418 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Zhou Y, et al. Flumethasone enhances the efficacy of chemotherapeutic drugs in lung cancer by inhibiting Nrf2 signaling pathway. Cancer Lett. 2020 Apr 1;474:94-105. [Content Brief]

[2]. Blake RL. Flumethasone induction of liver tyrosine aminotransferase activity in inbred strains and obese mutant mice. Biochem Pharmacol. 1970 May;19(5):1803-15. [Content Brief]

[3]. Bednarek D, et al. The effect of steroidal and non-steroidal anti-inflammatory drugs on the cellular immunity of calves with experimentally-induced local lung inflammation. Vet Immunol Immunopathol. 1999 Oct 1;71(1):1-15. [Content Brief]

[4]. Willi RA, et al. Environmental glucocorticoids corticosterone, betamethasone and flumethasone induce more potent physiological than transcriptional effects in zebrafish embryos. Sci Total Environ. 2019 Jul 1;672:183-191. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4364 mL	12.1818 mL	24.3635 mL	60.9088 mL
	5 mM	0.4873 mL	2.4364 mL	4.8727 mL	12.1818 mL
	10 mM	0.2436 mL	1.2182 mL	2.4364 mL	6.0909 mL
	15 mM	0.1624 mL	0.8121 mL	1.6242 mL	4.0606 mL
	20 mM	0.1218 mL	0.6091 mL	1.2182 mL	3.0454 mL
	25 mM	0.0975 mL	0.4873 mL	0.9745 mL	2.4364 mL
	30 mM	0.0812 mL	0.4061 mL	0.8121 mL	2.0303 mL
	40 mM	0.0609 mL	0.3045 mL	0.6091 mL	1.5227 mL
	50 mM	0.0487 mL	0.2436 mL	0.4873 mL	1.2182 mL
	60 mM	0.0406 mL	0.2030 mL	0.4061 mL	1.0151 mL
	80 mM	0.0305 mL	0.1523 mL	0.3045 mL	0.7614 mL
	100 mM	0.0244 mL	0.1218 mL	0.2436 mL	0.6091 mL

Keywords

Flumethasone

1 Cited Publications

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Flumethasone

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Publications Citing Use of MedChemExpress (MCE) Flumethasone

Voir tous les produits spécifiques à Isoform TNF Receptor

Voir tous les produits spécifiques à Isoform Interleukin Related

Activité biologique

Chemical Information

Publications (1)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Flumethasone Related Classifications

HY-B1051 Related Classifications