f-MKKFRW
f-MKKFRW is a formylated MgrB signal sequence peptide and Fpr3 agonist. f-MKKFRW can be produced by bacteria. f-MKKFRW is selectively recognized by a subset of vomeronasal sensory neurons and activates Fpr3-expressing and B2m-expressing VSNs, but not NP2-expressing VSNs. f-MKKFRW activates TRPM5-dependent signaling, triggering acetylcholine release. f-MKKFRW drives innate avoidance behavior requiring Fpr3, Gαo, and Trpc2.
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- Formule: C44H66N12O8S
- Masse moléculaire:923.14
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
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Activité biologique
Description
IC50 & Target
[2]|
TRPM5 |
In Vitro
f-MKKFRW (8 min) stimulates PTS in isolated mouse trachea through a chemosensory cell-dependent, TRPM5/PLCβ2/IP3R3-dependent, Atropine (HY-B1205)- and 4-DAMP-sensitive pathway[2].
f-MKKFRW (8 min) increases the ciliary beating frequency in isolated mouse trachea from 8.7 Hz to 14.2 Hz[2].
f-MKKFRW (50 µM) elicits transient Ca2+ elevation in a subset of TRPM5+ tracheal chemosensory cells (19/92; 21%)[2].
f-MKKFRW (10 µM; 15 min) triggers TRPM5-dependent acetylcholine release in isolated mouse trachea, which can be detected by M3 reporter cells and HPLC[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6N and transgenic lines (B6; ΔFpr3-lacZ, cGαo, Trpc2; adult male; 8-14 weeks old for behavioral tests; adult male for in-vivo VSN activation)[1]
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Dosage:100 μM in PBS; 100 μM final concentration in 50% FEU; 100 μM final concentration in FEU; 50 μM final concentration in FEU
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Administration:oronasal application; single exposure; free investigation/nasal contact; 10 min
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Result:In freely behaving mice, both oronasal application and free investigation at 100 μM induced Egr1 expression in approximately 50% of Fpr3-positive VSNs; this activation was not observed with control stimuli.
In behavioral tests, 100 μM elicited robust avoidance in B6 mice, ΔFpr3-lacZ+/+, cGαo+/−, and Trpc2+/− mice.
Mice still displayed avoidance at 50 μM.
Avoidance was absent in ΔFpr3-lacZ−/−, cGαo−/−, and Trpc2−/− mice.
Chemical Information
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Masse moléculaire 923.14
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Formule C44H66N12O8S
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Sequence
{fMet}-Lys-Lys-Phe-Arg-Trp
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Sequence Shortening
{fMet}-KKFRW
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
f-MKKFRW Related Classifications
HY-P11704 Related Classifications
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