CCR4 antagonist 4
Based on 1 publication(s) in Google Scholar
CCR4 antagonist 4 is a selective CCR4 antagonist with an IC50 of 0.02 μM that blocks MDC/CCL22-mediated signaling. CCR4 antagonist 4 blocks MDC-mediated chemotaxis and calcium mobilization and reduces eosinophil infiltration into the allergic pulmonary airway in mouse models. CCR4 antagonist 4 can be used for research on allergic pulmonary inflammation.
For research use only. We do not sell to patients.
- Purity : 98.92%
- CAS No.: 668980-17-4
- Formula: C24H27Cl2N7O
- Molecular Weight:500.42
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) CCR4 antagonist 4
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Biological Activity
Description
IC50 & Target
[1]|
CCR4 20 nM (IC50) |
MDC |
CCL22 |
In Vitro
CCR4 antagonist 4 blocks MDC-mediated chemotaxis with an IC50 of 0.007 μM[1].
CCR4 antagonist 4 is >500-fold more selective for CCR4 than for CCR3, CCR2, CXCR3, 5-HT1A, 5-HT6, and 5-HT7[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Mice pre-
viously immunized with Ovalbumin[1] -
Dosage:3, 10, and 30 mg/kg
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Administration:s.c.; twice daily
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Result:Reduced eosinophil recruitment with an ED50 of ~10 mg/kg.
At 30 mg/kg, was as efficacious as Dexamethasone (HY-14648) in reducing eosinophilic infiltration into mouse BAL.
Chemical Information
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CAS No. 668980-17-4
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Appearance Solid
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Molecular Weight 500.42
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Formula C24H27Cl2N7O
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Color White to off-white
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SMILES
ClC(C=C(C=C1)Cl)=C1CNC2=NC(N3CCN(CC3)C([C@H]4CCCCN4)=O)=NC5=C2C=CC=N5
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Commun Biol
Macrophages foster anti-tumor immunity by ZEB1-dependent cytotoxic T cell chemoattraction. [Abstract]2025 Jul 1;8(1):976. PMID: 40595293
Solvent & Solubility
In Vitro:
DMSO : ≥ 100 mg/mL (199.83 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.00 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.00 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.9983 mL | 9.9916 mL | 19.9832 mL | 49.9580 mL |
| 5 mM | 0.3997 mL | 1.9983 mL | 3.9966 mL | 9.9916 mL | |
| 10 mM | 0.1998 mL | 0.9992 mL | 1.9983 mL | 4.9958 mL | |
| 15 mM | 0.1332 mL | 0.6661 mL | 1.3322 mL | 3.3305 mL | |
| 20 mM | 0.0999 mL | 0.4996 mL | 0.9992 mL | 2.4979 mL | |
| 25 mM | 0.0799 mL | 0.3997 mL | 0.7993 mL | 1.9983 mL | |
| 30 mM | 0.0666 mL | 0.3331 mL | 0.6661 mL | 1.6653 mL | |
| 40 mM | 0.0500 mL | 0.2498 mL | 0.4996 mL | 1.2490 mL | |
| 50 mM | 0.0400 mL | 0.1998 mL | 0.3997 mL | 0.9992 mL | |
| 60 mM | 0.0333 mL | 0.1665 mL | 0.3331 mL | 0.8326 mL | |
| 80 mM | 0.0250 mL | 0.1249 mL | 0.2498 mL | 0.6245 mL | |
| 100 mM | 0.0200 mL | 0.0999 mL | 0.1998 mL | 0.4996 mL |
CCR4 antagonist 4 Related Classifications
HY-157453 Related Classifications
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