H2AX - H2A.X variant histone Gene

Also Known as H2A.X; H2A/X; H2AFX

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 3014

About H2AX

Cytogenetic location: 11q23.3 Genomic coordinates (GRCh38): 11:119,093,874-119,095,465 (from NCBI)

This gene has 2 transcripts (splice variants), 204 orthologues and 27 paralogues.

Summary

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015]

H2AX Products (1)

mRNA	Protein	Name
NM_002105.3	NP_002096.1	histone H2AX

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	Verweise	Source
enables chromatin-protein adaptor activity	IDA IDA: Inferred from direct assay	12419185	GOA
enables enzyme binding	IPI IPI: Inferred from physical interaction	19234442	GOA
enables histone binding	IPI IPI: Inferred from physical interaction	19234442	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	12419185	GOA

Biological Process GO Annotation	Evidence	Verweise	Source
involved in DNA damage checkpoint signaling	IDA IDA: Inferred from direct assay	17974976	GOA
involved in DNA damage response	IDA IDA: Inferred from direct assay	15149599	GOA
involved in DNA damage response	IMP IMP: Inferred from mutant phenotype	27248496	GOA
involved in protein localization to site of double-strand break	IDA IDA: Inferred from direct assay	12419185	GOA

Cellular Component GO Annotation	Evidence	Verweise	Source
located in centrosome	IDA IDA: Inferred from direct assay	17498979	GOA
colocalizes with chromosome, telomeric region	IDA IDA: Inferred from direct assay	15149599	GOA
located in nucleus	IDA IDA: Inferred from direct assay	11331621	GOA
located in nucleus	IMP IMP: Inferred from mutant phenotype	24507776	GOA
located in site of DNA damage	IMP IMP: Inferred from mutant phenotype	27248496	GOA
is active in site of double-strand break	IDA IDA: Inferred from direct assay	12419185	GOA
located in site of double-strand break	IDA IDA: Inferred from direct assay	21270334	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

H2AX Protein Structure

Histone

0
100
143 a.a.

Protein Preferred Names	Protein Names
histone H2AX	H2A histone family member X

H2AX Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	Verweise
Intra	H2AX	P16104	PPM1D	Homo sapiens	O15297	Anti Tag CoIP	20118229
Cross	H2AX	P16104	Eya3	Mus musculus	Q6P4T3	Anti Tag CoIP	19234442
Cross	H2AX	P16104	Eya3	Mus musculus	Q6P4T3	Phosphatase Assay	19234442
Intra	H2AX	P16104	VRK1	Homo sapiens	Q99986	Protein Kinase Assay	25923214
Intra	H2AX	P16104	MAPK8	Homo sapiens	P45983	Pull Down	19234442
Intra	H2AX	P16104	H4C16	Homo sapiens	P62805	Anti Tag CoIP	20000738
Intra	H2AX	P16104	H4C16	Homo sapiens	P62805	Anti Tag CoIP	33961781
Intra	H2AX	P16104	H4C16	Homo sapiens	P62805	Anti Tag CoIP	20224553
Intra	H2AX	P16104	H4C16	Homo sapiens	P62805	Anti Tag CoIP	28514442
Intra	H2AX	P16104	H4C16	Homo sapiens	P62805	TAP	24981860
Intra	H2AX	P16104	SMARCA4	Homo sapiens	P51532	Anti Tag CoIP	20224553
Intra	H2AX	P16104	BRCA1	Homo sapiens	P38398	IF	18001825
Intra	H2AX	P16104	BRCA1	Homo sapiens	P38398	Confocal	18001824
Intra	H2AX	P16104	LMNA	Homo sapiens	P02545	Anti Tag CoIP	23658700
Intra	H2AX	P16104	LMNA	Homo sapiens	P02545	Anti Tag CoIP	20000738
Intra	H2AX	P16104	LMNA	Homo sapiens	P02545	PLA	23658700
Intra	H2AX	P16104	H3-4	Homo sapiens	Q16695	Anti Tag CoIP	20224553
Intra	H2AX	P16104	MRE11	Homo sapiens	P49959	Imaging	12419185
Intra	H2AX	P16104	MRE11	Homo sapiens	P49959	Pull Down	19234442
Intra	H2AX	P16104	MRE11	Homo sapiens	P49959	Pull Down	16377563
Intra	H2AX	P16104	MRE11	Homo sapiens	P49959	CoIP	12419185
Intra	H2AX	P16104	MRE11	Homo sapiens	P49959	Pull Down	18583988
Intra	H2AX	P16104	TP53BP1	Homo sapiens	Q12888	IF	21317870
Intra	H2AX	P16104	TP53BP1	Homo sapiens	Q12888	Confocal	18001824
Intra	H2AX	P16104	H2BC5	Homo sapiens	P58876	Crosslink	30021884
Intra	H2AX	P16104	H2BC5	Homo sapiens	P58876	Anti Tag CoIP	33961781
Intra	H2AX	P16104	H2BC5	Homo sapiens	P58876	Anti Tag CoIP	28514442
Intra	H2AX	P16104	NBN	Homo sapiens	O60934	CoIP	12419185
Intra	H2AX	P16104	NBN	Homo sapiens	O60934	Pull Down	16377563
Intra	H2AX	P16104	NBN	Homo sapiens	O60934	IF	22565321
Intra	H2AX	P16104	NBN	Homo sapiens	O60934	Pull Down	18583988
Intra	H2AX	P16104	MDC1	Homo sapiens	Q14676	Imaging	12607005
Intra	H2AX	P16104	MDC1	Homo sapiens	Q14676	Pull Down	16377563
Intra	H2AX	P16104	MDC1	Homo sapiens	Q14676	Pull Down	18583988
Intra	H2AX	P16104	MDC1	Homo sapiens	Q14676	Anti Tag CoIP	19234442
Intra	H2AX	P16104	MDC1	Homo sapiens	Q14676	Pull Down	19234442
Intra	H2AX	P16104	MDC1	Homo sapiens	Q14676	X-Ray Diffraction	16377563
Cross	H2AX	P16104	Q9Z0X1-PRO_0000022031	Mus musculus	Q9Z0X1-PRO_0000022031	SPR	20360685
Intra	H2AX	P16104	TERF2	Homo sapiens	Q15554	Pull Down	21044950
Intra	H2AX	P16104	TERF2	Homo sapiens	Q15554	IF	21824286
Intra	H2AX	P16104	PAXIP1	Homo sapiens	Q6ZW49-1	FPS	22064073
Cross	H2AX	P16104	Apbb1	Rattus norvegicus	P46933-1	Anti Tag CoIP	19234442
Cross	H2AX	P16104	Apbb1	Rattus norvegicus	P46933-1	Pull Down	19234442

Cross: Cross-species interaction Intra: Intraspecies interaction

H2AX Antibodies

Art. -Nr.	Produktname	Anwendung	Reactivity
HY-P80161	Histone H2A.X Antibody (YA380)	WB, ICC/IF, IHC-P, IP	Human, Mouse, Rat
HY-P80463	Phospho-Histone H2A.X (Ser139) Antibody (YA190)	WB, IHC-P, ICC/IF, IF-Tissue	Human, Mouse, Rat, Monkey
HY-P80821	Phospho-Histone H2A.X (Ser139) Antibody (YA191)	WB, IHC-P, ICC/IF, IP	Human, Mouse, Rat
HY-P80821A	Phospho-Histone H2A.X (Ser139) Antibody (YA191)(PBS only)	WB, IP	Human, Mouse, Rat
HY-P80941	Phospho-Histone H2A.X (Ser139) Antibody (YA844)	WB, ICC/IF	Human, Mouse
HY-P80941A	Phospho-Histone H2A.X (Ser139) Antibody (YA844)(PBS only)	WB, ICC/IF	Human, Mouse
HY-P84123	H2A.X Antibody (YA3820)	WB, IHC-P, ICC/IF, FC	Human
HY-P84123A	H2A.X Antibody (YA3820)(PBS only)	WB, IHC-P, ICC/IF, FC	Human
HY-P86516	Phospho-Histone H2A.X(Ser139) Antibody (YA6208)	WB, IHC-P, ICC/IF, IP, ELISA	Human, Mouse, Rat

Related Diseases

Diseases	Alias
Nijmegen Breakage Syndrome	Berlin Breakage Syndrome NBS Microcephaly, Normal Intelligence And Immunodeficiency Ataxia-Telangiectasia Variant Ataxia-Telangiectasia Variant V1 Seemanova Syndrome Ii Immunodeficiency-Microcephaly-Chromosomal Instability Syndrome Seemanova Syndrome Type 2 At-V1 Microcephaly With Normal Intelligence, Immunodeficiency, And Lymphoreticular Malignancies Nonsyndromal Microcephaly, Autosomal Recessive, With Normal Intelligence Immunodeficiency, Microcephaly, And Chromosomal Instability Microcephaly-Immunodeficiency-Lymphoreticuloma Syndrome Microcephaly Immunodeficiency Lymphoreticuloma Microcephaly With Normal Intelligence Immunodeficiency And Lymphoreticular Malignancies Nonsyndromal Microcephaly Autosomal Recessive With Normal Intelligence Seemanova Syndrome 2 Ataxia-Telangiectasia Variant 1 Seemanova Syndrome At V1 Ataxia-Telangiectasia, Variant 1 Microcephaly-Immunodeficiency-Lymphoid Malignancy Syndrome V-At Ataxia Telangiectasia Variant V1
Ataxia-Telangiectasia	Ataxia Telangiectasia Louis-Bar Syndrome AT At1 Ataxia-Telangiectasia Syndrome Ataxia - Telangiectasia Variant Boder-Sedgwick Syndrome Louis Bar Syndrome Cerebello-Oculocutaneous Telangiectasia Immunodeficiency With Ataxia Telangiectasia A-T Ataxia Telangiectasia Syndrome Atm Telangiectasia, Cerebello-Oculocutaneous Ataxia-Telangiectasia Variant
Riddle Syndrome	RIDL Radiosensitivity-Immunodeficiency-Dysmorphic Features-Learning Difficulties Syndrome Rnf168 Deficiency Radiosensitivity, Immunodeficiency, Dysmorphic Facial Features, And Learning Difficulties Radiosensitivity, Immunodeficiency, Dysmorphic Features, And Learning Difficulties
Diffuse Large B-Cell Lymphoma	Dlbcl Diffuse Large B-Cell Lymphoma, Not Otherwise Specified Large B-Cell Diffuse Lymphoma Lymphoma, Large B-Cell, Diffuse Dlbcl - [Diffuse Large B-Cell Lymphoma] Diffuse Large Beta Cell Lymphoma
B-Cell Lymphoma	Lymphoma, B-Cell B-Cell Lymphomas B-Cell Lymphocytic Neoplasm Lymphoma B-Cell B-Cell Lymphoma Nos
Fanconi Anemia, Complementation Group A	Fanconi Anemia Fanconi Pancytopenia Fanconi Anemia Complementation Group A FANCA Fa Fanconi Panmyelopathy Fanconi'S Anemia Fanconi Anaemia Fanconi'S Anaemia Fanconi Hypoplastic Anemia Estren-Dameshek Variant Of Fanconi Anemia Estren-Dameshek Variant Of Fanconi Pancytopenia Fanconi Anemia Estren-Dameshek Variant Fanconis Anemia
Lymphoma	Lymphoid Cancer Lymphomas Lymphoid Cancers Lymphoid Neoplasm Lymphoma Nos Nhl - [Non-Hodgkin Lymphoma] Non-Hodgkin Lymphoma Non-Hodgkin Lymphoma, Nos Non-Hodgkin Malignant Lymphoma Nos
Epicardium Cancer	Malignant Neoplasm Of Epicardium Epicardial Tumor Malignant Epicardial Tumor Neoplasm Of Epicardium
Cornelia De Lange Syndrome	De Lange Syndrome Brachmann De Lange Syndrome Brachmann-De Lange Syndrome Cdls Bdls Typus Degenerativus Amstelodamensis
Autosomal Recessive Cerebellar Ataxia	Arca
Ovarian Cancer	Ovarian Carcinoma Ovarian Neoplasm Malignant Tumour Of Ovary Cancer Of The Ovary Epithelial Ovarian Cancer Neoplasm Of Ovary Ovarian Neoplasms Ovarian Cancers Malignant Neoplasm Of Ovary Primary Malignant Neoplasm Of Ovary Ovarian Cancer, Somatic Malignant Ovarian Tumor Ovary Neoplasm Primary Ovarian Cancer Tumor Of The Ovary Malignant Neoplasm Of The Ovary Malignant Tumor Of The Ovary Ovarian Malignant Tumor OC Ovarian Carcinomas Cancer, Ovarian Cancer Of Ovary Ovary Cancer Ca Ovary
Bone Cancer	Malignant Bone Neoplasm Bone Carcinoma Bone Neoplasms Bone Neoplasm Bone Tumour Ca - Bone Cancer Malignant Bone Tumour Malignant Neoplasm Of Bone Malignant Osseous Tumor Neoplasm Of Bone Osseous Tumor Osteosarcoma Of Bone Osteogenic Neoplasm
Xeroderma Pigmentosum, Variant Type	Xeroderma Pigmentosum XPV Xeroderma Pigmentosum Variant Type Xeroderma Pigmentosum With Normal Dna Repair Rates Photosensitivity With Defective Dna Synthesis Xp De Sanctis-Cacchione Syndrome Desanctis-Cacchione Syndrome Xeroderma Pigmentosa Xerodermic Idiocy Xeroderma Pigmentosum Variant Xp - [Xeroderma Pigmentosum] Atrophoderma Pigmentosum
Colorectal Cancer	Colon Cancer Colorectal Carcinoma Colon Carcinoma Colorectal Cancer, Susceptibility To Carcinoma Of Colon CRC Colorectal Cancer With Chromosomal Instability, Somatic Colon Cancer, Somatic Colon Cancer, Susceptibility To Colonic Neoplasms Colorectal Neoplasms Colorectal Cancer, Somatic Colon Cancer, Advanced, Somatic Colonic Carcinoma Colorectal Carcinomas Colon Cancers Colorectal Cancers Cancer, Colorectal, Somatic Cancer, Colon Cancer, Colorectal, Susceptibility To Colorectal Neoplasm Colonic Neoplasm Malignant Tumor Of Colon
Cerebellar Disease	Cerebellar Diseases Cerebellar Dysfunction Cerebellar Abnormality Cerebellar Disorders
Breast Cancer	Breast Carcinoma Male Breast Cancer Breast Cancer, Familial Malignant Neoplasm Of Breast Breast Cancer, Susceptibility To Breast Cancer, Early-Onset Malignant Tumor Of Breast Carcinoma Of Male Breast Breast Cancer, Invasive Ductal Breast Cancer, Protection Against Breast Cancer, Somatic Breast Cancer, Male Breast Cancer, Lobular, Somatic Breast Tumor Mammary Cancer Mammary Tumor Malignant Neoplasm Of Male Breast Mammary Carcinoma Male Breast Carcinoma Familial Cancer Of Breast Invasive Ductal Breast Carcinoma Breast Cancer Susceptibility Breast Cancer, Male, Susceptibility To Breast Cancer, Early-Onset, Susceptibility To Malignant Tumor Of The Breast Mammary Neoplasm Primary Breast Cancer Neoplasm Of Male Breast Carcinoma Of Breast Breast Cancer In Men Familial Breast Cancer Cancer Of Breast BC Breast Cancer Familial Breast Cancer Familial Male Breast Cancer, Familial Male Breast Male Carcinoma Breast Neoplasms Breast Neoplasms, Male Mammary Tumors Mammary Carcinomas Cancer, Breast Cancer, Breast, Susceptibility Invasive Breast Ductal Carcinoma Breast Neoplasm Susceptibility To Breast Cancer Mammary Neoplasms Animal Mammary Neoplasms Primary Malignant Neoplasm Of Breast Infiltrating Ductal Carcinoma Of Breast Infiltrating Duct Carcinoma Of Unspecified Site Infiltrating Ductular Carcinoma Of Unspecified Site Invasive Breast Carcinoma Of No Special Type Microinvasive Carcinoma Of Breast Carcinoma With Apocrine Differentiation
Aplastic Anemia	Aplastic Anemia, Susceptibility To Anemia Aplastic Idiopathic Aplastic Anemia Secondary Aplastic Anemia Idiopathic Bone Marrow Failure Aplastic Anemia Idiopathic AA Anemia, Aplastic Aplastic Anemia, Idiopathic Erythroid Aplasia Aa - [Aplastic Anaemia] Haematopoietic Aplasia Aleukia Haemorrhagica Anaemia Due To Decreased Red Cell Production Aplasia Bone Marrow Aplastic Bone Marrow Hypoplastic Anaemia Nos Myeloid Bone Marrow Aplasia Pancytopenia Panhaematopenia Hypoproliferative Anaemia Medullary Hypoplasia Red Blood Cells Hypoplastic Anaemia Panmyelophthisis Panhemocytopenia Refractive Hypoproliferative Anaemia Toxic Anaemia Toxic Aplastic Anaemia Aplastic Anaemia Due To Toxic Cause Idiopathic Aplastic Anaemia Nos
Hereditary Breast Ovarian Cancer Syndrome	Hereditary Breast And Ovarian Cancer Syndrome Brca1- And Brca2-Associated Hereditary Breast And Ovarian Cancer Breast And/Or Ovarian Cancer Breast And Ovarian Cancer Syndrome Hboc Syndrome Hereditary Breast And Ovarian Cancer Brca1- Brca2-Associated Hboc

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Approval
Clinical Phase
Pre-clinical Phase

Approval

Bioactive Molecules (1)

Art. -Nr.	Produktname	Wirkung	Reinheit	Rare Diseases
HY-113041	Prostaglandin A2		99.9%	Unkown

Clinical Phase

Bioactive Molecules (6)

Art. -Nr.	Produktname	Reinheit	Phase
HY-12037	Rigosertib sodium	99.57%	Phase 3
HY-18174	Prexasertib	99.64%	Phase 2
HY-18174A	Prexasertib dihydrochloride	99.73%	Phase 2
HY-18174E	Prexasertib dimesylate	98.90%	Phase 2
HY-18174B	Prexasertib Mesylate Hydrate	/	Phase 2
HY-18174C	Prexasertib mesylate	/	Phase 2

Pre-clinical Phase

Bioactive Molecules (35)

Art. -Nr.	Produktname	Wirkung	Reinheit	Rare Diseases
HY-117693	(E/Z)-Mirin	Inhibitor	99.22%	Unkown
HY-123834	FEN1-IN-1		99.85%	Unkown
HY-12037A	Rigosertib		99.01%	Unkown
HY-101089	RHPS4		99.88%	Unkown
HY-122181	OTS186935	Inhibitor	99.69%	Unkown
HY-158975	VRK1/CK1-IN-1	Inhibitor	98.69%	Unkown
HY-122181B	OTS186935 hydrochloride	Inhibitor	99.58%	Unkown
HY-W1126504	ETN029	Ligand	99.91%	Unkown
HY-N0316	Mollugin		99.79%	Unkown
HY-103241	Ro 90-7501		98.50%	Unkown
HY-163944	LL-K12-18		98.42%	Unkown
HY-122182	OTS193320		98.12%	Unkown
HY-N2445	Flavokawain C		99.79%	Unkown
HY-181557	SY-589		98.50%	Unkown
HY-RS05957	H2AX Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-122181A	OTS186935 trihydrochloride	Inhibitor	/	Unkown
HY-117688	WJ35435		/	Unkown
HY-121777	SFOM-0046	Inhibitor	/	Unkown
HY-122860	SKLB-C05		/	Unkown
HY-132972	TrxR-IN-2		/	Unkown
HY-145288	Antitumor agent-36		/	Unkown
HY-145289	Antitumor agent-37		/	Unkown
HY-162985	JHD205	Inhibitor	/	Unkown
HY-167237	Calactin		/	Unkown
HY-168877	FMP	Activator	/	Unkown
HY-174989	ATM-IN-2		/	Unkown
HY-175700	YCJ-02		/	Unkown
HY-181018	Topo I/DDX5-IN-1		/	Unkown
HY-181678	DNA-PK/HDAC6-IN-1		/	Unkown
HY-183330	Topo I/II-IN-3		/	Unkown
HY-184416	GDD-261		/	Unkown
HY-W414334	ICR-191 dihydrochloride	Activator	≥95.0%	Unkown
HY-18174H	Prexasertib lactate		/	Unkown
HY-RS16644	H2ax Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS23078	H2ax Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Macaca mulatta	H2AX	VGNC	VGNC:109694
Felis catus	H2AX	VGNC	VGNC:109892
Rattus norvegicus	H2AX	RGD	RGD:1566119
Bos taurus	H2AX	VGNC	VGNC:83617
Mus musculus	H2AX	MGD	MGI:102688
Canis familiaris	H2AX	VGNC	VGNC:83734
Others	H2AX	NCBI