Cat. No.: HY-18260

Bisphenol A

16 Cited Publications

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Based on 16 publication(s) in Google Scholar

Bisphenol A is a phenolic, organic synthetic compound widely used in the production of polycarbonate plastics and epoxy resins. Bisphenol A is a reproductive, developmental, and systemic toxicant, often classified as an endocrine-disrupting compound (EDC). Bisphenol A is associated with many diseases, including cancers, cardiovascular diseases, respiratory diseases, diabetes, kidney diseases, obesity, and reproductivedisorders.

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Pureté: 99.91%
CAS No.: 80-05-7
Formule: C₁₅H₁₆O₂
Masse moléculaire:228.29

Stockage:

Store at room temperature 3 years.

In solvent -80°C, 2 years , -20°C, 1 year

Bisphenol A Chemical Structure

CAS No.: 80-05-7

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Activité biologique
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (16)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

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Cellular Effect

Cell Line	Type	Value	Description	References
B16	IC₅₀	0.0086 M Compound: BAP	Compound was tested for the in vitro growth inhibition of B-16 melanoma cells after incubation for 3 days (cytotoxicity), the dose that failed to inhibit cell growth by more than 50% was determined Compound was tested for the in vitro growth inhibition of B-16 melanoma cells after incubation for 3 days (cytotoxicity), the dose that failed to inhibit cell growth by more than 50% was determined	[PMID: 9288164]
HEK293	EC₅₀	1689 nM Compound: 3; BPA	Selective estrogen receptor down-regulator activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ERalpha degradation by luciferase reporter gene assay Selective estrogen receptor down-regulator activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ERalpha degradation by luciferase reporter gene assay	[PMID: 30940565]
HEK293	EC₅₀	>10000 nM Compound: 3; BPA	Agonist activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ER-alpha-mediated transcriptional activity by luciferase reporter gene assay Agonist activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ER-alpha-mediated transcriptional activity by luciferase reporter gene assay	[PMID: 30940565]
HEK293	IC₅₀	>10000 nM Compound: 3; BPA	Antagonist activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as reduction in E2-induced ER-alpha-mediated transcriptional activity by luciferase reporter gene assay Antagonist activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as reduction in E2-induced ER-alpha-mediated transcriptional activity by luciferase reporter gene assay	[PMID: 30940565]
HeLa	EC₅₀	757 nM Compound: BPA	Agonist activity at ERbeta (unknown origin) expressed in human HeLa cells assessed as transcriptional activation measured after 24 hrs by luciferase reporter gene assay Agonist activity at ERbeta (unknown origin) expressed in human HeLa cells assessed as transcriptional activation measured after 24 hrs by luciferase reporter gene assay	[PMID: 31879182]
HeLa	EC₅₀	797 nM Compound: BPA	Agonist activity at ERalpha (unknown origin) expressed in human HeLa cells assessed as transcriptional activation measured after 24 hrs by luciferase reporter gene assay Agonist activity at ERalpha (unknown origin) expressed in human HeLa cells assessed as transcriptional activation measured after 24 hrs by luciferase reporter gene assay	[PMID: 31879182]
HT-22	IC₅₀	97 μM Compound: 33	Cytotoxicity against mouse HT-22 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay Cytotoxicity against mouse HT-22 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay	[PMID: 36876904]
MCF7	EC₅₀	0.29 μM Compound: 4; BPA	Agonist activity at estrogen receptor in human MCF7 cells assessed as ERE-mediated transcriptional activity after 24 hrs by luciferase assay Agonist activity at estrogen receptor in human MCF7 cells assessed as ERE-mediated transcriptional activity after 24 hrs by luciferase assay	[PMID: 26905830]

In Vitro

Bisphenol A significantly reduces the viability, increases and decreases levels of intracellular ROS production and antioxidant capacity, respectively, in KGN cells^[1].
Bisphenol A (100 fM-100 μM, 24-72 h) decreases granulosa cell viability and induces apoptosis and G2-to-M arrest of ovarian granulosa cells^[3].
Bisphenol A (0-400 μM, 24 h) suppresses proliferation and induces apoptosis in human colon cancer cells HCT116 through mitochondrial and MAPK/AKT pathways^[4].
Bisphenol A (0.5-12.5 μM, 5 days) significantly inhibits RANKL-induced, TRAP-positive multinucleated cell formation in bone marrow-derived macrophages and RAW 264.7 cell cultures^[5].
Bisphenol A (0.5-12.5 μM) suppresses ALP activities and bone nodule formation in MC3T3-E1 cells^[5].
Bisphenol A (0.06-500 μM, 1 h) induces morphological and biochemical alterations in human peripheral blood mononuclear cells^[6].
Bisphenol A (0.0020-2.0 μM, 24-48 h) decreases viability and induces dysfunction of insulin secretion and apoptosis through the damage of mitochondria in rat insulinoma (INS-1) cells^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[5]

Cell Line:

RAW264.7 cells
Concentration:

0.5, 2.5, 12.5 μM
Incubation Time:

24 h
Result:

Significantly reduced to 80% at 12.5 μM.

Apoptosis Analysis^[5]

Cell Line:

RAW264.7 cells
Concentration:

0.5, 2.5, 12.5 μM
Incubation Time:

24 h
Result:

Stimulated apoptosis in a dose-dependent manner in both cells.
Up-regulated Bax and down-regulated Bcl2 in both cells.

In Vivo

Bisphenol A (200 mg/kg/day, s.c., 4 weeks) significantly decreases testis, epididymis, prostate and seminal vesicle weights, and the testicular daily sperm production in Jcl:Wistar rats^[8].
Bisphenol A (500-1250 mg/kg/day, gastric intubation, gestational days 6 through 15) induces maternal mortality and fetal toxicity in CD-1 mice^[9].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 80-05-7
Appearance Solid
Masse moléculaire 228.29
Formule C₁₅H₁₆O₂
Color White to off-white
SMILES

CC(C1=CC=C(O)C=C1)(C2=CC=C(O)C=C2)C
Structure Classification
- Phenols
- Polyphenols
Initial Source
- Endogenous metabolite
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Publications (16)

All WBIn Vivo Efficacy StudyBio/Physico-chemical AssayHistological Imaging/Staining

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Journal Impact Factor

Environ Sci Technol

Bisphenol A Disrupts Spermatogenesis via Excessive Mitophagy-Driven Ferroptosis. [Abstract]

2025 Oct 28;59(42):22423-22438. PMID: 41104744

Bisphenol A purchased from MedChemExpress. Usage Cited in: Environ Sci Technol. 2025 Oct 28;59(42):22423-22438. [Abstract]

Bisphenol A (BPA) (20-2000 μg/kg; i.g.; once daily for 4 weeks) significantly downregulated GPX4 expression in the testes of mice in a dose-dependent manner.

Bisphenol A purchased from MedChemExpress. Usage Cited in: Environ Sci Technol. 2025 Oct 28;59(42):22423-22438. [Abstract]

Bisphenol A (BPA) (20-2000 μg/kg; i.g.; once daily for 4 weeks) induced testicular atrophy and significantly decreased the index of the mice testis.

Bisphenol A purchased from MedChemExpress. Usage Cited in: Environ Sci Technol. 2025 Oct 28;59(42):22423-22438. [Abstract]

Bisphenol A (BPA) (20-2000 μg/kg; i.g.; once daily for 4 weeks) increased iron and malondialdehyde (MDA) content while significantly decreased the GSH/GSSG ratio in mice testis.

Bisphenol A purchased from MedChemExpress. Usage Cited in: Environ Sci Technol. 2025 Oct 28;59(42):22423-22438. [Abstract]

Histological changes and TEM imaging images of mitochondria structures in the testes of Bisphenol A (BPA) (20-2000 μg/kg; i.g.; once daily for 4 weeks)-treated mice. NST, normal seminiferous tubule; DST, damaged seminiferous tubule; LC, Leydig cells; Sg, spermatogonia. M, mitochondria; LC, loss of mitochondrial cristae; rOM, ruptured mitochondrial outer membrane; AL, autolysosome; SM, shrunken mitochondria.

Bisphenol A purchased from MedChemExpress. Usage Cited in: Environ Sci Technol. 2025 Oct 28;59(42):22423-22438. [Abstract]

Bisphenol A (BPA) (20-2000 μg/kg; i.g.; once daily for 4 weeks) significantly upregulated the protein expression of both estrogen receptors ERα and ERβ, as well as the G protein-coupled estrogen receptor (GPER) in mice testes, in a dose-dependent manner.
Biosens Bioelectron

Highly sensitive detection for endocrine disruptors using human cell biosensors expressing red fluorescence protein mScarlet3. [Abstract]

2026 Sep 1:307:118716. PMID: 42070441
Environ Int

2026 Mar 28.
Free Radic Biol Med

Bisphenol A induces placental ferroptosis and fetal growth restriction via the YAP/TAZ-ferritinophagy axis. [Abstract]

2023 Dec 14:211:127-144. PMID: 38103660
Sci Total Environ

New insights into the effect of bisphenol AF exposure on maternal mammary glands at various stages of gestation in mice. [Abstract]

2022 Dec 1:850:157793. PMID: 35934037
Environ Pollut

Comparative effects of genistein and bisphenol A on non-alcoholic fatty liver disease in laying hens. [Abstract]

2021 Nov 1:288:117795. PMID: 34274649
Environ Pollut

Environmental level bisphenol A accelerates alterations of the reno-cardiac axis by the MAPK cascades in male diabetic rats: An analysis based on transcriptomic profiling and bioinformatics. [Abstract]

2021 Oct 15:287:117671. PMID: 34435562
Environ Health (Wash)

Fish Skin Mucus Vitellogenin as a Noninvasive, Sensitive Biomarker for Aquatic Xenoestrogens. [Abstract]

2025 Jan 10;3(4):414-424. PMID: 40270534
J Agric Food Chem

Co-Exposure to Polystyrene Microplastics and Bisphenol A Contributes to the Formation of Liver Fibrosis in Mice through Inhibition of the BMAL1/E-Cad Signaling Pathway. [Abstract]

2025 Mar 26;73(12):7405-7422. PMID: 40073227
Ecotoxicol Environ Saf

Association of maternal bisphenol exposure during pregnancy with spontaneous preterm birth and preliminary mechanistic exploration in a prospective cohort. [Abstract]

2026 May 14:318:120237. PMID: 42134009
Ecotoxicol Environ Saf

Bisphenol A exacerbates osteoblast ferroptosis via the p53/SLC7A11 axis: A novel mechanistic insight into environmental osteoporosis pathogenesis. [Abstract]

2025 Dec 13:309:119560. PMID: 41391364
Ecotoxicol Environ Saf

Prenatal BPA exposure disrupts androgen synthesis in testicular Leydig cells via epigenetic modifications of steroidogenic factor 1. [Abstract]

2025 Nov 15:307:119409. PMID: 41252971
Ecotoxicol Environ Saf

Revealing the role of bisphenol A on prostate cancer progression and identifying potential targets: A comprehensive analysis from population cohort to molecular mechanism. [Abstract]

2025 Apr 17:296:118209. PMID: 40249974
Biofactors

Naringenin protects swine testis cells from bisphenol A-induced apoptosis via Keap1/Nrf2 signaling pathway. [Abstract]

2022 Jan;48(1):190-203. PMID: 34914851
Food Chem Toxicol

Rapamycin protects Sertoli cells against BPA-induced autophagy disorders. [Abstract]

2024 Apr:186:114510. PMID: 38365117
SSRN

2025 Aug 31.

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (438.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.3804 mL

21.9020 mL

43.8039 mL

5 mM

0.8761 mL

4.3804 mL

8.7608 mL

10 mM

0.4380 mL

2.1902 mL

4.3804 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (9.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (9.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (9.11 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

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Références

[1]. Huang M, et al. Bisphenol A and its analogues bisphenol S, bisphenol F and bisphenol AF induce oxidative stress and biomacromolecular damage in human granulosa KGN cells. Chemosphere. 2020 Apr 9;253:126707. [Content Brief]

[2]. Rubin BS, et al. Bisphenol A: an endocrine disruptor with widespread exposure and multiple effects. J Steroid Biochem Mol Biol. 2011 Oct;127(1-2):27-34. [Content Brief]

[3]. Xu J, et al. Bisphenol A induces apoptosis and G2-to-M arrest of ovarian granulosa cells. Biochem Biophys Res Commun. 2002 Mar 29;292(2):456-62. [Content Brief]

[4]. Qu W, et al. Bisphenol A suppresses proliferation and induces apoptosis in colonic epithelial cells through mitochondrial and MAPK/AKT pathways. Life Sci. 2018 Sep 1;208:167-174. [Content Brief]

[5]. Hwang JK, et al. Bisphenol A reduces differentiation and stimulates apoptosis of osteoclasts and osteoblasts. Life Sci. 2013 Sep 17;93(9-11):367-72. [Content Brief]

[6]. Michałowicz J, et al. Bisphenol A and its analogs induce morphological and biochemical alterations in human peripheral blood mononuclear cells (in vitro study). Toxicol In Vitro. 2015 Oct;29(7):1464-72. [Content Brief]

[7]. Lin Y, et al. Exposure to bisphenol A induces dysfunction of insulin secretion and apoptosis through the damage of mitochondria in rat insulinoma (INS-1) cells. Cell Death Dis. 2013 Jan 17;4(1):e460. [Content Brief]

[8]. Takahashi O, et al. Testicular toxicity of dietarily or parenterally administered bisphenol A in rats and mice. Food Chem Toxicol. 2003 Jul;41(7):1035-44. [Content Brief]

[9]. Morrissey RE, et al. The developmental toxicity of bisphenol A in rats and mice. Fundam Appl Toxicol. 1987 May;8(4):571-82. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.3804 mL	21.9020 mL	43.8039 mL	109.5098 mL
	5 mM	0.8761 mL	4.3804 mL	8.7608 mL	21.9020 mL
	10 mM	0.4380 mL	2.1902 mL	4.3804 mL	10.9510 mL
	15 mM	0.2920 mL	1.4601 mL	2.9203 mL	7.3007 mL
	20 mM	0.2190 mL	1.0951 mL	2.1902 mL	5.4755 mL
	25 mM	0.1752 mL	0.8761 mL	1.7522 mL	4.3804 mL
	30 mM	0.1460 mL	0.7301 mL	1.4601 mL	3.6503 mL
	40 mM	0.1095 mL	0.5475 mL	1.0951 mL	2.7377 mL
	50 mM	0.0876 mL	0.4380 mL	0.8761 mL	2.1902 mL
	60 mM	0.0730 mL	0.3650 mL	0.7301 mL	1.8252 mL
	80 mM	0.0548 mL	0.2738 mL	0.5475 mL	1.3689 mL
	100 mM	0.0438 mL	0.2190 mL	0.4380 mL	1.0951 mL

Keywords

Bisphenol A

16 Cited Publications

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Bisphenol A

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Publications Citing Use of MedChemExpress (MCE) Bisphenol A

Voir tous les produits spécifiques à Isoform Akt

Voir tous les produits spécifiques à Isoform Endogenous Metabolite

Voir tous les produits spécifiques à Isoform p38 MAPK

Activité biologique

Chemical Information

Publications (16)

Solvant et solubilité

Pureté et documentation

Références