VPC01091.4
Based on 1 Customer Validation
VPC01091.4 (VPC4) is a TRPM7 inhibitor and blocks TRPM7 current at low micromolar concentrations. VPC01091.4 can penetrate the blood-brain barrier. VPC01091.4 is an efficacious anti-inflammatory agent that arrests systemic inflammation in vivo.
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- Pureté: 98.04%
- CAS No.: 945604-76-2
- Formule: C20H33NO
- Masse moléculaire:303.48
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Immunology/Inflammation Compound Library
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Mechanoreceptors Compound Library
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Pain-Related Compound Library
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Membrane Protein-targeted Compound Library
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Membrane Receptor-targeted Compound Library
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Ion Channel Compound Library
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Bioactive Compound Library Max
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MCE Bioactive Compound Library
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High-Throughput Bioactive Compound Library
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Activité biologique
VPC01091.4 (0.1-10 µM) effectively suppresses TRPM7 current (IC50 = 0.665 µ M) in HEK 293T cells[1].
VPC01091.4 (0.1, 1, 5, 10 µM, Pre treatment for 10 minutes and LPS (HY-D1056) stimulation for 3 hours) can reduce the expression of inflammatory factors in macrophages[1].
VPC01091.4 (0.1-25 μM, 24 h) has no significant toxicity at concentrations ≤ 10 µM and mild toxicity at 25 µM in HeLa cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RAW 264.7 cells, BMDMs or mouse primary alveolar macrophages
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Concentration:0.1, 1, 5, 10 µM
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Incubation Time:Pre treatment for 10 minutes and LPS stimulation for 3 hours
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Result:Significantly inhibited the expression of IL-1β, TNFα, CCL4, and IL-6.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:LPS induced C57BL/6J mice (12 weeks)[1]
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Dosage:30 mg/kg
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Administration:Intraperitoneal injection (i.p.), single dose
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Result:Significantly reduced the elevation of IL-1β, IFNγ, TNFα caused by LPS, and increased IL-10 levels.
Its concentration in the brain was about 9 times that of blood, and in the lungs it was about 32 times.
Significantly inhibited the expression of IL-1 β, IL-6, TNF α, and CCL4 in brain and lung tissues.
Reduced clinical symptom scores, delayed weight loss, and improved the survival rate of mice.
Chemical Information
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CAS No. 945604-76-2
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Appearance Solid
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Masse moléculaire 303.48
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Formule C20H33NO
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Color White to off-white
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SMILES
CCCCCCCCC1=CC=C([C@@H]2CC[C@@](CO)(C2)N)C=C1
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Synonyms
VPC4
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
DMSO : 100 mg/mL (329.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (269 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2951 mL | 16.4755 mL | 32.9511 mL | 82.3777 mL |
| 5 mM | 0.6590 mL | 3.2951 mL | 6.5902 mL | 16.4755 mL | |
| 10 mM | 0.3295 mL | 1.6476 mL | 3.2951 mL | 8.2378 mL | |
| 15 mM | 0.2197 mL | 1.0984 mL | 2.1967 mL | 5.4918 mL | |
| 20 mM | 0.1648 mL | 0.8238 mL | 1.6476 mL | 4.1189 mL | |
| 25 mM | 0.1318 mL | 0.6590 mL | 1.3180 mL | 3.2951 mL | |
| 30 mM | 0.1098 mL | 0.5492 mL | 1.0984 mL | 2.7459 mL | |
| 40 mM | 0.0824 mL | 0.4119 mL | 0.8238 mL | 2.0594 mL | |
| 50 mM | 0.0659 mL | 0.3295 mL | 0.6590 mL | 1.6476 mL | |
| 60 mM | 0.0549 mL | 0.2746 mL | 0.5492 mL | 1.3730 mL | |
| 80 mM | 0.0412 mL | 0.2059 mL | 0.4119 mL | 1.0297 mL | |
| 100 mM | 0.0330 mL | 0.1648 mL | 0.3295 mL | 0.8238 mL |
VPC01091.4 Related Classifications
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