Cat. No.: HY-N0705

Curculigoside

9 Cited Publications

Customer Review

Based on 9 publication(s) in Google Scholar

Curculigoside is the main saponin in C. orchioide, exerts significant antioxidant, anti-osteoporosis, antidepressant and neuroprotection effects. Curculigoside possesses significant anti-arthritic effects in vivo and in vitro via regulation of the JAK/STAT/NF-κB signaling pathway.

For research use only. We do not sell to patients.

Purity: 99.82%
CAS No.: 85643-19-2
Formula: C₂₂H₂₆O₁₁
Molecular Weight:466.44

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Curculigoside Chemical Structure

CAS No.: 85643-19-2

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (9)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

JAK; STAT; NF-κB^[1]

All JAK Isoforms :

All STAT Isoforms :

All NF-κB Isoforms :

In Vitro

Curculigoside (1-64 μg/ml; 72 hours) exerts significant inhibitory effects on MH7A cell viability^[1].
? Curculigoside (4-16 μg/ml; 24 hours) decreases the protein expression of JAK1, JAK3 and STAT3 compared to the TNF-α group^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

MH7A cells
Concentration:

1 μg/ml; 2 μg/ml; 4 μg/ml; 8 μg/ml; 16 μg/ml; 32 μg/ml; 64 μg/ml
Incubation Time:

72 hours
Result:

Had inhibitory effect on MH7A cells.

Western Blot Analysis^[1]

Cell Line:

MH7A cells
Concentration:

4 μg/ml; 64 μg/ml
Incubation Time:

24 hours
Result:

Downregulated JAK1, JAK3 and STAT3 protein expression.

Chemical Information

CAS No. 85643-19-2
Appearance Solid
Molecular Weight 466.44
Formula C₂₂H₂₆O₁₁
Color White to off-white
SMILES

O=C(C(C(OC)=CC=C1)=C1OC)OCC(C=C(O)C=C2)=C2O[C@@H]([C@@H]([C@@H](O)[C@@H]3O)O)O[C@@H]3CO
Structure Classification
- Phenols
- Monophenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (9)

All Histological Imaging/StainingELISAWBIFFlow Cytometry

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Journal Impact Factor

Osteoarthritis Cartilage

Curculigoside upregulates BMAL1 to decrease nucleus pulposus cell apoptosis by inhibiting the JAK/STAT3 pathway. [Abstract]

2025 Apr;33(4):412-425. PMID: 39622432
Cell Commun Signal

Nesfatin-1 enhances vascular smooth muscle calcification through facilitating BMP-2 osteogenic signaling. [Abstract]

2024 Oct 11;22(1):488. PMID: 39394127
Int J Mol Med

Curculigoside mitigates dextran sulfate sodium‑induced colitis by activation of KEAP1‑NRF2 interaction to inhibit oxidative damage and autophagy of intestinal epithelium barrier. [Abstract]

2023 Nov;52(5):107. PMID: 37772380

Curculigoside purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2023 Nov;52(5):107. [Abstract]

Colon morphology was analyzed by hematoxylin and eosin staining treated with Curculigoside (CUR) (10, 20 mg/kg, i.g.).

Curculigoside purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2023 Nov;52(5):107. [Abstract]

Serum were determined by ELISA treated with Curculigoside (CUR) (10, 20 mg/kg, i.g.).

Curculigoside purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2023 Nov;52(5):107. [Abstract]

Caco2 cells were treated with Curculigoside (CUR) (10, 20, 30, 40 μM) for 4 h followed by TNF-α (100 ng/ml) for 24 h. The protein levels of ZO-1 and occludin were assessed by western blot.

Curculigoside purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2023 Nov;52(5):107. [Abstract]

Mitochondrial membrane potential was detected using JC‑1 probe treated with Curculigoside (CUR) (20, 40 μM).

Curculigoside purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2023 Nov;52(5):107. [Abstract]

Caco2 cell ROS levels were measured by DCFHDA ROS assay kit treated with Curculigoside (CUR) (20, 40 μM).
J Ethnopharmacol

Efficacy of curculigoside in protecting against ischemic brain injury through regulation of oxidative stress and NF-κB and PI3K/Akt expression. [Abstract]

2023 Jan 30:301:115804. PMID: 36228892
Life Sci

Curculigoside inhibits ferroptosis in ulcerative colitis through the induction of GPX4. [Abstract]

2020 Oct 15;259:118356. PMID: 32861798
Hum Exp Toxicol

Curculigoside mitigates hepatic ischemia/reperfusion-induced oxidative stress, inflammation, and apoptosis via activation of the Nrf-2/HO-1 pathway. [Abstract]

Jan-Dec 2022;41:9603271221087146. PMID: 35331031
J Orthop Surg Res

Curculigoside promotes osteogenic differentiation of ADSCs to prevent ovariectomized-induced osteoporosis. [Abstract]

2021 Apr 26;16(1):279. PMID: 33902663
Tissue Cell

Curculigoside exhibits multiple therapeutic efﬁcacy to induce apoptosis and ferroptosis in osteosarcoma via modulation of ROS and tumor microenvironment. [Abstract]

2025 Jan 16:93:102745. PMID: 39864205
Acta Cir Bras

Corynoline alleviates renal ischemia-reperfusion injury by enhancing Nrf2/HO-1 pathway. [Abstract]

2025 Aug 18:40:e406825. PMID: 40834223

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (214.39 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 2 mg/mL (4.29 mM)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1439 mL

10.7195 mL

21.4390 mL

5 mM

0.4288 mL

2.1439 mL

4.2878 mL

10 mM

0.2144 mL

1.0719 mL

2.1439 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.46 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (4.46 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (4.46 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Tan S, et al. Curculigoside exerts significant anti arthritic effects in vivo and in vitro via regulation of the JAK/STAT/NF κB signaling pathway. Mol Med Rep. 2019 Mar;19(3):2057-2064. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	2.1439 mL	10.7195 mL	21.4390 mL	53.5975 mL
DMSO	5 mM	0.4288 mL	2.1439 mL	4.2878 mL	10.7195 mL
	10 mM	0.2144 mL	1.0719 mL	2.1439 mL	5.3597 mL
	15 mM	0.1429 mL	0.7146 mL	1.4293 mL	3.5732 mL
	20 mM	0.1072 mL	0.5360 mL	1.0719 mL	2.6799 mL
	25 mM	0.0858 mL	0.4288 mL	0.8576 mL	2.1439 mL
	30 mM	0.0715 mL	0.3573 mL	0.7146 mL	1.7866 mL
	40 mM	0.0536 mL	0.2680 mL	0.5360 mL	1.3399 mL
	50 mM	0.0429 mL	0.2144 mL	0.4288 mL	1.0719 mL
	60 mM	0.0357 mL	0.1787 mL	0.3573 mL	0.8933 mL
	80 mM	0.0268 mL	0.1340 mL	0.2680 mL	0.6700 mL
	100 mM	0.0214 mL	0.1072 mL	0.2144 mL	0.5360 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Curculigoside

9 Cited Publications

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Curculigoside

Epigenetics Compound Library

Immunology/Inflammation Compound Library

JAK/STAT Compound Library

Kinase Inhibitor Library

NF-κB Signaling Compound Library

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Publications Citing Use of MedChemExpress (MCE) Curculigoside

All JAK Isoforms

All STAT Isoforms

All NF-κB Isoforms

Biological Activity

Chemical Information

Publications (9)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Curculigoside Related Classifications

HY-N0705 Related Classifications