2. Epigenetics Stem Cell/Wnt Protein Tyrosine Kinase/RTK JAK/STAT Signaling NF-κB
  3. JAK STAT NF-κB
  4. Curculigoside

Curculigoside is the main saponin in C. orchioide, exerts significant antioxidant, anti-osteoporosis, antidepressant and neuroprotection effects. Curculigoside possesses significant anti-arthritic effects in vivo and in vitro via regulation of the JAK/STAT/NF-κB signaling pathway.

For research use only. We do not sell to patients.

CAS No. : 85643-19-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Based on 9 publication(s) in Google Scholar

Other Forms of Curculigoside:

Curculigoside Related Antibodies

Top Publications Citing Use of Products

    Curculigoside purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2023 Nov;52(5):107.  [Abstract]

    Colon morphology was analyzed by hematoxylin and eosin staining treated with Curculigoside (CUR) (10, 20 mg/kg, i.g.).

    Curculigoside purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2023 Nov;52(5):107.  [Abstract]

    Serum were determined by ELISA treated with Curculigoside (CUR) (10, 20 mg/kg, i.g.).

    Curculigoside purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2023 Nov;52(5):107.  [Abstract]

    Caco2 cells were treated with Curculigoside (CUR) (10, 20, 30, 40 μM) for 4 h followed by TNF-α (100 ng/ml) for 24 h. The protein levels of ZO-1 and occludin were assessed by western blot.

    Curculigoside purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2023 Nov;52(5):107.  [Abstract]

    Mitochondrial membrane potential was detected using JC‑1 probe treated with Curculigoside (CUR) (20, 40 μM).

    Curculigoside purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2023 Nov;52(5):107.  [Abstract]

    Caco2 cell ROS levels were measured by DCFHDA ROS assay kit treated with Curculigoside (CUR) (20, 40 μM).

    View All JAK Isoform Specific Products:

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    JAK1 JAK2 JAK3 Tyk2 JAK

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    STAT3 STAT5 STAT6 STAT1

    View All NF-κB Isoform Specific Products:

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    NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Curculigoside is the main saponin in C. orchioide, exerts significant antioxidant, anti-osteoporosis, antidepressant and neuroprotection effects. Curculigoside possesses significant anti-arthritic effects in vivo and in vitro via regulation of the JAK/STAT/NF-κB signaling pathway[1].

    IC50 & Target

    JAK; STAT; NF-κB[1]
    In Vitro

    Curculigoside (1-64 μg/ml; 72 hours) exerts significant inhibitory effects on MH7A cell viability[1].
    ? Curculigoside (4-16 μg/ml; 24 hours) decreases the protein expression of JAK1, JAK3 and STAT3 compared to the TNF-α group[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Curculigoside Related Antibodies

    Cell Viability Assay[1]

    Cell Line: MH7A cells
    Concentration: 1 μg/ml; 2 μg/ml; 4 μg/ml; 8 μg/ml; 16 μg/ml; 32 μg/ml; 64 μg/ml
    Incubation Time: 72 hours
    Result: Had inhibitory effect on MH7A cells.

    Western Blot Analysis[1]

    Cell Line: MH7A cells
    Concentration: 4 μg/ml; 64 μg/ml
    Incubation Time: 24 hours
    Result: Downregulated JAK1, JAK3 and STAT3 protein expression.
    Molecular Weight

    466.44

    Formula

    C22H26O11
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(C(C(OC)=CC=C1)=C1OC)OCC(C=C(O)C=C2)=C2O[C@@H]([C@@H]([C@@H](O)[C@@H]3O)O)O[C@@H]3CO
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (214.39 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : ≥ 2 mg/mL (4.29 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1439 mL 10.7195 mL 21.4390 mL
    5 mM 0.4288 mL 2.1439 mL 4.2878 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

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    Concentration (final)

    C2

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (4.46 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (4.46 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.82%

    SDS (252 KB)

    COA (254 KB) HNMR (283 KB) LCMS (94 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 2.1439 mL 10.7195 mL 21.4390 mL 53.5975 mL
    DMSO 5 mM 0.4288 mL 2.1439 mL 4.2878 mL 10.7195 mL
    10 mM 0.2144 mL 1.0719 mL 2.1439 mL 5.3597 mL
    15 mM 0.1429 mL 0.7146 mL 1.4293 mL 3.5732 mL
    20 mM 0.1072 mL 0.5360 mL 1.0719 mL 2.6799 mL
    25 mM 0.0858 mL 0.4288 mL 0.8576 mL 2.1439 mL
    30 mM 0.0715 mL 0.3573 mL 0.7146 mL 1.7866 mL
    40 mM 0.0536 mL 0.2680 mL 0.5360 mL 1.3399 mL
    50 mM 0.0429 mL 0.2144 mL 0.4288 mL 1.0719 mL
    60 mM 0.0357 mL 0.1787 mL 0.3573 mL 0.8933 mL
    80 mM 0.0268 mL 0.1340 mL 0.2680 mL 0.6700 mL
    100 mM 0.0214 mL 0.1072 mL 0.2144 mL 0.5360 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Curculigoside Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Curculigoside85643-19-2JAKSTATNF-κBJanus kinaseNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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