Endoproteinase Asp-N (MS grade)

Product Information
Asp-N protease is a recombinantly expressed metalloprotease from Pseudomonas fragi bacteria with a molecular weight of 22.4 kDa. It has optimal activity at pH 7.5-8.5. The product contains trehalose and Tris-HCl and can be used for protein sequencing characterization and proteomics research.

Instructions
1. Reconstitution: 20 μL ultrapure water can be used to reconstitute (0.1 mg/mL). After dissolution, it can be divided and stored at -20°C to avoid repeated freezing and thawing. It can be stored for 1-2 weeks.
2. Enzyme digestion
1) Applicable enzyme digestion buffer solution: 20 mmol Tris, 50 mmol HEPES buffer; pH value is in the range of 7.5-8.5
2) Reaction conditions: 1:10-1:50 (w/w, enzyme: denatured protein sample), the protein concentration of the enzyme digestion reaction is controlled at 0.2-0.5 μg/μL, and incubated at 37°C for 2 h.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.