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Cat. No.: HY-P11704

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f-MKKFRW

f-MKKFRW is a formylated MgrB signal sequence peptide and Fpr3 agonist. f-MKKFRW can be produced by bacteria. f-MKKFRW is selectively recognized by a subset of vomeronasal sensory neurons and activates Fpr3-expressing and B2m-expressing VSNs, but not NP2-expressing VSNs. f-MKKFRW activates TRPM5-dependent signaling, triggering acetylcholine release. f-MKKFRW drives innate avoidance behavior requiring Fpr3, Gαo, and Trpc2.

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화학식: C₄₄H₆₆N₁₂O₈S
분자량:923.14

보관:

Please store the product under the recommended conditions in the Certificate of Analysis.

f-MKKFRW Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
순도&문서
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

IC₅₀ & Target

^[2]

TRPM5

All Endogenous Metabolite Isoforms :

All TRP Channel Isoforms :

In Vitro

Bacterial InfectionOthers

Bacterial Infection

f-MKKFRW (3 μM) selectively activates HEK293T cells expressing Fpr3, and MgrB-H6 bacterial supernatant containing the MgrB-derived fMet peptide elicits a strong Fpr3-dependent Ca²⁺ response^[1].

Others

f-MKKFRW (1 μM) activates Fpr3-dependent Ca²⁺responses in VSNs of mouse VNO whole mounts, with a detection threshold ≤0.1 μM and a response proportion of approximately 0.15%^[1].
f-MKKFRW (8 min) stimulates PTS in isolated mouse trachea through a chemosensory cell-dependent, TRPM5/PLCβ2/IP3R3-dependent, Atropine (HY-B1205)- and 4-DAMP-sensitive pathway^[2].
f-MKKFRW (8 min) increases the ciliary beating frequency in isolated mouse trachea from 8.7 Hz to 14.2 Hz^[2].
f-MKKFRW (50 µM) elicits transient Ca²⁺ elevation in a subset of TRPM5⁺ tracheal chemosensory cells (19/92; 21%)^[2].
f-MKKFRW (10 µM; 15 min) triggers TRPM5-dependent acetylcholine release in isolated mouse trachea, which can be detected by M3 reporter cells and HPLC^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

f-MKKFRW (50-100 μM; oronasal administration/free exploration; single exposure) activates Fpr3-expressing vomeronasal sensory neurons in vivo and drives Fpr3-dependent innate avoidance behavior in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6N and transgenic lines (B6; ΔFpr3-lacZ, cGαo, Trpc2; adult male; 8-14 weeks old for behavioral tests; adult male for in-vivo VSN activation)^[1]
Dosage:

100 μM in PBS; 100 μM final concentration in 50% FEU; 100 μM final concentration in FEU; 50 μM final concentration in FEU
Administration:

oronasal application; single exposure; free investigation/nasal contact; 10 min
Result:

In freely behaving mice, both oronasal application and free investigation at 100 μM induced Egr1 expression in approximately 50% of Fpr3-positive VSNs; this activation was not observed with control stimuli.
In behavioral tests, 100 μM elicited robust avoidance in B6 mice, ΔFpr3-lacZ+/+, cGαo+/−, and Trpc2+/− mice.
Mice still displayed avoidance at 50 μM.
Avoidance was absent in ΔFpr3-lacZ−/−, cGαo−/−, and Trpc2−/− mice.

Chemical Information

분자량 923.14
화학식 C₄₄H₆₆N₁₂O₈S
Sequence

{fMet}-Lys-Lys-Phe-Arg-Trp
Sequence Shortening

{fMet}-KKFRW
선적

Room temperature in continental US; may vary elsewhere.
보관

Please store the product under the recommended conditions in the Certificate of Analysis.

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (291 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Bufe B, et al. Bacterial MgrB peptide activates chemoreceptor Fpr3 in mouse accessory olfactory system and drives avoidance behaviour. Nature communications. 2019 Oct 25;10(1):4889. [Content Brief]

[2]. Perniss A, et al. Chemosensory Cell-Derived Acetylcholine Drives Tracheal Mucociliary Clearance in Response to Virulence-Associated Formyl Peptides. Immunity. 2020 Apr 14;52(4):683-699.e11.

[3]. Haga-Yamanaka S, et al. From Receptors to Behavior: Molecular and Functional Logic of Sensory Coding in the Mouse Accessory Olfactory System. Chem Senses. 2026 Jun 24:bjag017.

Calculators

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

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Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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Amount: USD 0.00

Biological Activity
- 제품 설명
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
순도&문서
References

상품명:
Cat. No.:
수량:
MCE Japan Authorized Agent:

상품명:
CAS No.:
Species:
Purity:
수량:

f-MKKFRW

All Endogenous Metabolite Isoforms

All TRP Channel Isoforms