Duocarmycin TM
Based on 1 publication(s) in Google Scholar
Duocarmycin TM (CBI-TMI) is a potent antitumor antibiotic. Duocarmycin TM induces a sequence-selective alkylation of duplex DNA.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.82%
- No. CAS: 157922-77-5
- Fòrmula: C25H23ClN2O5
- Peso molecular:466.91
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Duocarmycin TM
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Actividad biológica
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Duocarmycins |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CHO-AA8 | IC50 |
0.21 nM
Compound: 33 (CBI-TMI)
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In vitro cellular toxicity against chinese hamster AA8 cell line after 4 hr of compound exposure
In vitro cellular toxicity against chinese hamster AA8 cell line after 4 hr of compound exposure
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[PMID: 12747786] |
| EMT6 | IC50 |
0.13 nM
Compound: 33 (CBI-TMI)
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In vitro cellular toxicity against murine mammary carcinoma EMT6 cell line after 4 hr of compound exposure
In vitro cellular toxicity against murine mammary carcinoma EMT6 cell line after 4 hr of compound exposure
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[PMID: 12747786] |
| SK-OV-3 | IC50 |
0.29 nM
Compound: 33 (CBI-TMI)
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In vitro cellular toxicity against human ovarian carcinoma SKOV3 cell line after 4 hr of compound exposure
In vitro cellular toxicity against human ovarian carcinoma SKOV3 cell line after 4 hr of compound exposure
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[PMID: 12747786] |
| UV4 | IC50 |
0.09 nM
Compound: 33 (CBI-TMI)
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In vitro cellular toxicity against chinese hamster UV4 cell line after 4 hr of compound exposure
In vitro cellular toxicity against chinese hamster UV4 cell line after 4 hr of compound exposure
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[PMID: 12747786] |
Duocarmycin TM (60 μM; 4 d; BJAB and WSU-DLCL2 cells) is a cytotoxic agent that inhibits the proliferation of tumor cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:BJAB and WSU-DLCL2 cells
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Concentration:60 μM
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Incubation Time:4 days
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Result:Inhibited the proliferation of tumor cells with IC50 values of 0.153 μM and 0.079μM for BJAB and WSU-DLCL2 cells, respectively.
Chemical Information
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No. CAS 157922-77-5
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Appearance Solid
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Peso molecular 466.91
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Fòrmula C25H23ClN2O5
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Color Light yellow to yellow
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SMILES
ClC[C@H]1C2=C(C=CC=C3)C3=C(O)C=C2N(C(C4=CC(C=C(OC)C(OC)=C5OC)=C5N4)=O)C1
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Synonyms
CBI-TMI
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Cell Chem Biol
A non-reactive natural product precursor of the duocarmycin family has potent and selective antimalarial activity. [Abstract]2022 May 19;29(5):840-853.e6. PMID: 34710358
Solvente y solubilidad
DMSO : ≥ 50 mg/mL (107.09 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.35 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1417 mL | 10.7087 mL | 21.4174 mL | 53.5435 mL |
| 5 mM | 0.4283 mL | 2.1417 mL | 4.2835 mL | 10.7087 mL | |
| 10 mM | 0.2142 mL | 1.0709 mL | 2.1417 mL | 5.3544 mL | |
| 15 mM | 0.1428 mL | 0.7139 mL | 1.4278 mL | 3.5696 mL | |
| 20 mM | 0.1071 mL | 0.5354 mL | 1.0709 mL | 2.6772 mL | |
| 25 mM | 0.0857 mL | 0.4283 mL | 0.8567 mL | 2.1417 mL | |
| 30 mM | 0.0714 mL | 0.3570 mL | 0.7139 mL | 1.7848 mL | |
| 40 mM | 0.0535 mL | 0.2677 mL | 0.5354 mL | 1.3386 mL | |
| 50 mM | 0.0428 mL | 0.2142 mL | 0.4283 mL | 1.0709 mL | |
| 60 mM | 0.0357 mL | 0.1785 mL | 0.3570 mL | 0.8924 mL | |
| 80 mM | 0.0268 mL | 0.1339 mL | 0.2677 mL | 0.6693 mL | |
| 100 mM | 0.0214 mL | 0.1071 mL | 0.2142 mL | 0.5354 mL |
Duocarmycin TM Related Classifications
HY-107769 Related Classifications
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