RN-1734
Based on 10 publication(s) in Google Scholar
RN-1734 is selective antagonist of the TRPV4 channel, completely antagonizes 4αPDD-mediated activation of TRPV4 with comparable, low micromolar IC50s for all three species (hTRPV4: 2.3 μM, mTRPV4: 5.9 μM, rTRPV4: 3.2 μM). RN-1734 clearly decreases the production of tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) without altering the number of olig2-positive cells.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.75%
- No. CAS: 946387-07-1
- Fòrmula: C14H22Cl2N2O2S
- Peso molecular:353.31
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
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Publications Citing Use of MedChemExpress (MCE) RN-1734
More- Bioact Mater. 2025 Nov 25:57:646-659. [Abstract]
- Adv Sci (Weinh). 2024 Dec 5:e2410360. [Abstract]
- Acs Biomater Sci Eng. 2019 Dec 9;5(12):6520-6529. [Abstract]
- Life Sci. 2024 Jan 1:336:122326. [Abstract]
- Eur J Pharmacol. 2025 Sep 15:1003:177900. [Abstract]
- Cell Calcium. 2026 May 23:136:103155.
- Cell Calcium. 2023 May:111:102715. [Abstract]
- J Biol Chem. 2022 May;298(5):101847. [Abstract]
- PLoS One. 2019 Jun 10;14(6):e0217511. [Abstract]
- Biol Bull. 2022 Feb;242(1):48-61. [Abstract]
Customer Validation & Images
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WB
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IF
Actividad biológica
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hTRPV4 2.3 μM (IC50) |
rTRPV4 3.2 μM (IC50) |
mTRPV4 5.9 μM (IC50) |
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Cell Line
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Type | Value | Description | References |
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| HEK293 | IC50 |
2.3 μM
Compound: RN1734
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Antagonist activity at human TRPV4 expressed in HEK293 cells assessed as inhibition of 4alpha-PDD-induced intracellular calcium after 10 mins in presence of 45Ca2+ by microbeta liquid scintillation counting method
Antagonist activity at human TRPV4 expressed in HEK293 cells assessed as inhibition of 4alpha-PDD-induced intracellular calcium after 10 mins in presence of 45Ca2+ by microbeta liquid scintillation counting method
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[PMID: 28523109] |
RN-1734 (27 hours; 10 μM) reverses the increase in the apoptotic rate of oligodendrocytes induced by CM (LPS-activated microglia group) apoptosis[2].
RN-1734 (27 hours; 10 μM) alleviates CM-induced decreases in CNP[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Microglial cells
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Concentration:27 hours
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Incubation Time:10μM
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Result:Significantly decreased the percentage of cleaved-caspase 3 positive cells.
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Cell Line:Microglial cells
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Concentration:27 hours
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Incubation Time:10 μM
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Result:Alleviated CM (with LPS only)-induced decreases in CNP.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:CPZ-induced demyelination mouse model (C57BL/6 male mice)[2]
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Dosage:0.5 μl (10 μM in 5% DMSO and 0.9% NaCl)
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Administration:Microinjector pump for 5 weeks
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Result:Significantly reversed the decrease in CNP protein and improved myelination in CPZ-induced demyelination mouse.
Chemical Information
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No. CAS 946387-07-1
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Appearance Solid
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Peso molecular 353.31
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Fòrmula C14H22Cl2N2O2S
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Color White to off-white
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SMILES
O=S(C1=CC=C(Cl)C=C1Cl)(N(C(C)C)CCNC(C)C)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (10)
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Journal Impact Factor
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Most Recent
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Bioact Mater
Food-derived β-lactoglobulin nanofibrils: An efficacy, safe, and scalable solution to overcome oral insulin delivery challenges. [Abstract]2025 Nov 25:57:646-659. PMID: 41377894 -
Adv Sci (Weinh)
Targeted SPP1 Inhibition of Tumor-Associated Myeloid Cells Effectively Decreases Tumor Sizes. [Abstract]2024 Dec 5:e2410360. PMID: 39639496 -
Acs Biomater Sci Eng
Cation Channel Transient Receptor Potential Vanilloid 4 Mediates Topography-Induced Osteoblastic Differentiation of Bone Marrow Stem Cells. [Abstract]2019 Dec 9;5(12):6520-6529. PMID: 33417804
RN-1734 purchased from MedChemExpress. Usage Cited in: Acs Biomater Sci Eng. 2019 Dec 9;5(12):6520-6529. [Abstract]
Protein levels of NFATc1 in nuclei of BMSCs cultured on various surfaces with or without GSK101 and RN-1734
RN-1734 purchased from MedChemExpress. Usage Cited in: Acs Biomater Sci Eng. 2019 Dec 9;5(12):6520-6529. [Abstract]
Effects of TRPV4 on NFATc1 nuclear localization in BMSCs on MNTs. Micrographs stained for NFATc1 of BMSCs cultured for 7d on (A) PT, (B) PT with TRPV4 agonist (GSK101) , (C) TNT-30, (D)TNT-100, (E) TNT-100 with TRPV4 antagonist (RN-1734). Red fluorescence: NFATc1; Blue fluorescence: nucleus.
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Life Sci
Regulatory role of Piezo1 channel in endothelium-dependent hyperpolarization-mediated vasorelaxation of small resistance vessels and its anti-inflammatory action. [Abstract]2024 Jan 1:336:122326. PMID: 38056769 -
Eur J Pharmacol
Novel mechanisms of metformin-induced vasorelaxation of mesenteric arterioles via endothelium-dependent hyperpolarization to treat murine colitis. [Abstract]2025 Sep 15:1003:177900. PMID: 40617384 -
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Cell Calcium
The Ca2+ channel TRPV4 is dispensable for Ca2+ influx and cell volume regulation during hypotonic stress response in human keratinocyte cell lines. [Abstract]2023 May:111:102715. PMID: 36933289 -
J Biol Chem
Capsaicin inhibits intestinal Cl- secretion and promotes Na+ absorption by blocking TRPV4 channels in healthy and colitic mice. [Abstract]2022 May;298(5):101847. PMID: 35314195 -
PLoS One
2019 Jun 10;14(6):e0217511. PMID: 31181129 -
Biol Bull
Functional Characterization of TRPV-Like Ion Channels Involved in Nematocyst Discharge from the Sea Anemone Diadumene lineata. [Abstract]2022 Feb;242(1):48-61. PMID: 35245164
Solvente y solubilidad
DMSO : 25 mg/mL (70.76 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3.25 mg/mL (9.20 mM); Clear solution
This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3.25 mg/mL (9.20 mM); Clear solution
This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Kato K, et al. Acidosis environment promotes osteoclast formation by acting on the last phase of preosteoclast differentiation: a study to elucidate the action points of acidosis and search for putative target molecules. Eur J Pharmacol. 2011 Aug 1;663(1-3):27-39. [Content Brief]
[2]. Liu M, et al. TRPV4 Inhibition Improved Myelination and Reduced Glia Reactivity and Inflammation in a Cuprizone-Induced Mouse Model of Demyelination. Front Cell Neurosci. 2018 Nov 5;12:392. [Content Brief]
[3]. Vincent F, et al. Identification and characterization of novel TRPV4 modulators. Biochem Biophys Res Commun. 2009 Nov 20;389(3):490-4. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8304 mL | 14.1519 mL | 28.3038 mL | 70.7594 mL |
| 5 mM | 0.5661 mL | 2.8304 mL | 5.6608 mL | 14.1519 mL | |
| 10 mM | 0.2830 mL | 1.4152 mL | 2.8304 mL | 7.0759 mL | |
| 15 mM | 0.1887 mL | 0.9435 mL | 1.8869 mL | 4.7173 mL | |
| 20 mM | 0.1415 mL | 0.7076 mL | 1.4152 mL | 3.5380 mL | |
| 25 mM | 0.1132 mL | 0.5661 mL | 1.1322 mL | 2.8304 mL | |
| 30 mM | 0.0943 mL | 0.4717 mL | 0.9435 mL | 2.3586 mL | |
| 40 mM | 0.0708 mL | 0.3538 mL | 0.7076 mL | 1.7690 mL | |
| 50 mM | 0.0566 mL | 0.2830 mL | 0.5661 mL | 1.4152 mL | |
| 60 mM | 0.0472 mL | 0.2359 mL | 0.4717 mL | 1.1793 mL |
RN-1734 Related Classifications
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