DHPG
Based on 4 publication(s) in Google Scholar
DHPG is the agonist for mGluR 1/5 (EC50 for mGluR 1 is 60 nM) that activates the phospholipase C (PLC) pathway, and leads ultimately to the activation of protein kinase C (PKC).
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.60%
- No. CAS: 146255-66-5
- Fòrmula: C8H9NO4
- Peso molecular:183.16
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Almacenamiento:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) DHPG
More
Customer Validation & Images
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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WB
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In Vivo Efficacy Study
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WB
Actividad biológica
Descripciòn
IC50 & Target
[1]|
mGluR 1 60 nM (EC50) |
mGluR 5 |
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Rat chronic constriction injury (CCI) models[3]
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Dosage:25 nM
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Administration:intrathecal injection, single dose
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Result:Induced plantar touching and licking behaviors in mice.
Chemical Information
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No. CAS 146255-66-5
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Appearance Solid
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Peso molecular 183.16
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Fòrmula C8H9NO4
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Color Gray to brown
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SMILES
O=C(O)C(N)C1=CC(O)=CC(O)=C1
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Synonyms
(RS)-3,5-DHPG
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Publications (4)
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Journal Impact Factor
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Most Recent
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Neurochem Int
Red nucleus mGluR1 and mGluR5 facilitate the development of neuropathic pain through stimulating the expressions of TNF-α and IL-1β. [Abstract]2024 Sep:178:105786. PMID: 38843952
DHPG purchased from MedChemExpress. Usage Cited in: Neurochem Int. 2024 Sep:178:105786. [Abstract]
Administration of mGluR I agonist DHPG (5.0 μg) to the RN provoked a significant mechanical allodynia in normal rats.
DHPG purchased from MedChemExpress. Usage Cited in: Neurochem Int. 2024 Sep:178:105786. [Abstract]
Administration of mGluR1 antagonist LY367385 (5.0 μg) to the RN at 10 min after DHPG (5.0 μg) administration ameliorated DHPG-induced mechanical allodynia.
DHPG purchased from MedChemExpress. Usage Cited in: Neurochem Int. 2024 Sep:178:105786. [Abstract]
Western blotting displayed that administration of mGluR I agonist DHPG (5.0 μg) to the RN enhanced the protein expression of TNF-α in normal rats, this effect could be blocked by LY367385 or MTEP.
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iScience
Celsr3 is required for Purkinje cell maturation and regulates cerebellar postsynaptic plasticity. [Abstract]2021 Jul 1;24(7):102812. PMID: 34308297
DHPG purchased from MedChemExpress. Usage Cited in: iScience. 2021 Jul 1;24(7):102812. [Abstract]
To explore whether Celsr3 regulates mGluR1 signaling during LTD induction, we perfused acute cerebellar slices with the mGluR1 selective agonist DHPG. As predicted, in control slices, DHPG (100 μM) efficiently induced PF-PC LTD, while the PPR was unaffected. By contrast, in Celsr3 cKO slices, DHPG perfusion failed to induce LTD in PCs.
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Neuroscience
Artesunate Reduces Remifentanil-induced Hyperalgesia and Peroxiredoxin-3 Hyperacetylation via Modulating Spinal Metabotropic Glutamate Receptor 5 in Rats. [Abstract]2022 Apr 1:487:88-98. PMID: 35026318
DHPG purchased from MedChemExpress. Usage Cited in: Neuroscience. 2022 Apr 1:487:88-98. [Abstract]
Induction of acute pain by mGluR5 agonist injection and attenuation of allodynia by artesunate therapy in rats. Artesunate (intrathecal, 100 μg) was injected 10 min before mGluR5 agonist DHPG (intrathecal, 1 nmol) intervention. Spinal artesunate therapy drastically alleviated DHPG-induced mechanical allodynia and thermal hyperalgesia.
DHPG purchased from MedChemExpress. Usage Cited in: Neuroscience. 2022 Apr 1:487:88-98. [Abstract]
Western blot identified spinal levels of mGluR5 expression and peroxiredoxin-3 acetylation after DHPG (intrathecal, 1 nmol) injection and artesunate treatment.
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Neurosci Lett
Intracellular metabotropic glutamate receptor 5 in spinal dorsal horn neurons contributes to pain in a mouse model of vincristine-induced neuropathic pain. [Abstract]2025 Mar 15:852:138193. PMID: 40074023
Solvente y solubilidad
In Vitro:
DMSO : 33.33 mg/mL (181.97 mM; ultrasonic and adjust pH to 2 with HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 2 mg/mL (10.92 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.65 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.65 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Winder DG, et al. Metabotropic glutamate receptor (mGluR)-mediated potentiation of cyclic AMP responses does not require phosphoinositide hydrolysis: mediation by a group II-like mGluR. J Neurochem. 1995 Feb;64(2):592-9. [Content Brief]
[2]. Pellegrini-Giampietro DE, et al. Pharmacological characterization of metabotropic glutamate receptors coupled to phospholipase D in the rat hippocampus. Br J Pharmacol. 1996 Jun;118(4):1035-43. [Content Brief]
[3]. Scotland PE, et al., Enhanced 3,5-dihydroxyphenylglycine-induced sustained nociceptive behaviors in rats with neuropathy or chronic inflammation. Behav Brain Res. 2007 Dec 3;184(2):150-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 5.4596 mL | 27.2981 mL | 54.5962 mL | 136.4905 mL |
| 5 mM | 1.0919 mL | 5.4596 mL | 10.9192 mL | 27.2981 mL | |
| 10 mM | 0.5460 mL | 2.7298 mL | 5.4596 mL | 13.6490 mL | |
| DMSO | 15 mM | 0.3640 mL | 1.8199 mL | 3.6397 mL | 9.0994 mL |
| 20 mM | 0.2730 mL | 1.3649 mL | 2.7298 mL | 6.8245 mL | |
| 25 mM | 0.2184 mL | 1.0919 mL | 2.1838 mL | 5.4596 mL | |
| 30 mM | 0.1820 mL | 0.9099 mL | 1.8199 mL | 4.5497 mL | |
| 40 mM | 0.1365 mL | 0.6825 mL | 1.3649 mL | 3.4123 mL | |
| 50 mM | 0.1092 mL | 0.5460 mL | 1.0919 mL | 2.7298 mL | |
| 60 mM | 0.0910 mL | 0.4550 mL | 0.9099 mL | 2.2748 mL | |
| 80 mM | 0.0682 mL | 0.3412 mL | 0.6825 mL | 1.7061 mL | |
| 100 mM | 0.0546 mL | 0.2730 mL | 0.5460 mL | 1.3649 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
DHPG Related Classifications
HY-12598A Related Classifications
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