UNC10415667

UNC10415667 potently induces polyubiquitination of purified NSD2 PWWP1 domain via SCF-FBXO22 in vitro^[1].
UNC10415667 (1 µM; 6 h) potently degrades NSD2 in U2OS NSD2 HiBiT cells with a DC₅₀ of 460 nM, achieving ~75% degradation at 1 µM over 6 hours^[1].
UNC10415667 mediates formation of a stable ternary complex between purified FBXO22 FIST domain and NSD2 PWWP1 domain in a Cys326-dependent manner^[1].
UNC10415667 binds purified FBXO22 FIST domain in a Cys326-dependent manner, as demonstrated by a significant increase in the domain's melting temperature^[1].
UNC10415667 forms a stable covalent ternary complex with SCF-FBXO22 and NSD2 PWWP1 domain, adopting a conformation nearly identical to that of UNC10088C^[1].
UNC10415667 mediates positive cooperative binding between purified FBXO22 FIST domain and NSD2 PWWP1 domain, as measured by TR-FRET displacement^[1].
UNC10415667 mediates formation of a stable quaternary complex containing purified FBXO22 FIST domain, NSD2 PWWP1 domain, and BACH1 BTB domain, indicating simultaneous binding of both neosubstrate and native substrate to FBXO22^[1].
UNC10415667 (10 µM; 6 h) completely degrades NSD2 in HEK293T cells at 10 µM over 6 hours without perturbing BACH1 levels, and simultaneous degradation of NSD2 and native FBXO22 substrate BACH1 is achievable when combined with Hemin (HY-19424)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .