LIMK1 - LIM domain kinase 1 Gene

Also Known as LIMK; LIMK-1

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 3984

About LIMK1

Cytogenetic location: 7q11.23 Genomic coordinates (GRCh38): 7:74,083,804-74,122,525 (from NCBI)

This gene has 8 transcripts (splice variants), 262 orthologues, 23 paralogues and is associated with 1 phenotype. Ubiquitous expression in brain (RPKM 15.7), spleen (RPKM 11.2) and 25 other tissues.

Summary

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Feb 2011]

LIMK1 Products (3)

mRNA	Protein	Name
NM_016735.1
NM_002314.4	NP_002305.1	LIM domain kinase 1 isoform 1
NM_001204426.2	NP_001191355.1	LIM domain kinase 1 isoform 2

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables heat shock protein binding	IDA IDA: Inferred from direct assay	17512523	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	8940095	GOA
enables protein serine/threonine kinase activity	IDA IDA: Inferred from direct assay	22328514	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in negative regulation of ubiquitin-protein transferase activity	IDA IDA: Inferred from direct assay	17512523	GOA
involved in positive regulation of actin filament bundle assembly	IDA IDA: Inferred from direct assay	17512523	GOA
involved in protein phosphorylation	IDA IDA: Inferred from direct assay	17512523	GOA
involved in protein phosphorylation	IMP IMP: Inferred from mutant phenotype	23633677	GOA

Cellular Component GO Annotation	Evidence	References	Source
located in cytoplasm	IDA IDA: Inferred from direct assay	17512523	GOA
is active in glutamatergic synapse	IDA IDA: Inferred from direct assay	25884247	GOA
is active in glutamatergic synapse	IMP IMP: Inferred from mutant phenotype	25884247	GOA
is active in postsynapse	IDA IDA: Inferred from direct assay	25884247	GOA
is active in postsynapse	IMP IMP: Inferred from mutant phenotype	25884247	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

LIMK1 Protein Structure

LIM

PDZ

Pkinase_Tyr

0
100
200
300
400
500
600
647 a.a.

Protein Preferred Names	Protein Names
LIM domain kinase 1	LIM motif-containing protein kinase

LIMK1 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	LIMK1	P53667	PAK2	Homo sapiens	Q13177	NMR	16837009
Intra	LIMK1	P53667	C22orf15	Homo sapiens	Q8WYQ4-2	Y2H Prey Pooling	32296183
Intra	LIMK1	P53667	C22orf15	Homo sapiens	Q8WYQ4-2	Validated Y2H	32296183
Intra	LIMK1	P53667	C22orf15	Homo sapiens	Q8WYQ4-2	Y2H Array	32296183
Intra	LIMK1	P53667	SSH1	Homo sapiens	Q8WYL5	Phosphatase Assay	15660133
Intra	LIMK1	P53667	PAK1	Homo sapiens	Q13153	Pull Down	10559936
Intra	LIMK1	P53667	PAK1	Homo sapiens	Q13153	Anti Bait CoIP	10559936
Intra	LIMK1	P53667	CFL1	Homo sapiens	P23528	Protein Kinase Assay	17853892
Intra	LIMK1	P53667	CFL1	Homo sapiens	P23528	Protein Kinase Assay	19424295
Intra	LIMK1	P53667	LATS1	Homo sapiens	O95835	CoIP	15220930
Intra	LIMK1	P53667	LATS1	Homo sapiens	O95835	Imaging	15220930
Intra	LIMK1	P53667	MMP14	Homo sapiens	P50281	Anti Bait CoIP	21219645
Cross	LIMK1	P53667	Prkcg	Rattus norvegicus	P63319	Anti Tag CoIP	8940095
Cross	LIMK1	P53667	Prkcg	Rattus norvegicus	P63319	Anti Bait CoIP	8940095
Cross	LIMK1	P53667	Arrb2	Rattus norvegicus	P29067	Anti Tag CoIP	17500066
Cross	LIMK1	P53667	Arrb1	Rattus norvegicus	P29066	Anti Tag CoIP	17500066

Cross: Cross-species interaction Intra: Intraspecies interaction

LIMK1 Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P81941	LIM Kinase 1 Antibody (YA1686)	WB	Human, Mouse
HY-P85864	LIM Kinase 1+2 Antibody (YA5556)	WB, ICC/IF, ELISA	Human, Mouse, Rat

Related Diseases

Diseases	Alias
Prostate Cancer	Prostate Carcinoma Prostate Cancer, Familial Prostate Neoplasm Prostate Cancer, Somatic Prostate Cancer, Susceptibility To Prostatic Cancer Prostatic Neoplasms Hereditary Prostate Cancer Prostatic Neoplasm Cancer Of Prostate Carcinoma Of Prostate Familial Prostate Cancer Familial Prostate Carcinoma Malignant Tumor Of Prostate Malignant Neoplasm Of Prostate Prostate Cancer, Familial, Susceptibility To Malignant Tumor Of The Prostate Ngp - New Growth Of Prostate Tumor Of The Prostate Prostate Cancer, Hereditary Cancer Of The Prostate Malignant Neoplasm Of The Prostate Prostatic Carcinoma PC Prca Cancer, Prostate Malignant Prostatic Tumour Malignant Tumour Of Prostate Primary Prostate Cancer Primary Malignant Neoplasm Of Prostate Prostate Gland Cancer
Williams-Beuren Syndrome	Williams Syndrome WBS Wms Deletion 7q11.23 Monosomy 7q11.23 Chromosome 7q11.23 Deletion Syndrome, 1.5- To 1.8-Mb Fanconi Schlesinger Syndrome Beuren Syndrome Elfin Facies Syndrome Elfin Facies With Hypercalcemia Hypercalcemia-Supravalvar Aortic Stenosis Ws
Breast Cancer	Breast Carcinoma Breast Cancer, Familial Malignant Neoplasm Of Breast Male Breast Cancer Breast Cancer, Susceptibility To Breast Cancer, Early-Onset Malignant Tumor Of Breast Carcinoma Of Male Breast Breast Cancer, Invasive Ductal Breast Cancer, Protection Against Breast Cancer, Somatic Breast Cancer, Male Breast Cancer, Lobular, Somatic Breast Tumor Mammary Cancer Mammary Tumor Malignant Neoplasm Of Male Breast Mammary Carcinoma Male Breast Carcinoma Familial Cancer Of Breast Invasive Ductal Breast Carcinoma Breast Cancer Susceptibility Breast Cancer, Male, Susceptibility To Breast Cancer, Early-Onset, Susceptibility To Malignant Tumor Of The Breast Mammary Neoplasm Primary Breast Cancer Neoplasm Of Male Breast Carcinoma Of Breast Breast Cancer In Men Familial Breast Cancer Cancer Of Breast BC Breast Cancer Familial Breast Cancer Familial Male Breast Cancer, Familial Male Breast Male Carcinoma Breast Neoplasms Breast Neoplasms, Male Mammary Tumors Mammary Carcinomas Cancer, Breast Cancer, Breast, Susceptibility Invasive Breast Ductal Carcinoma Breast Neoplasm Susceptibility To Breast Cancer Mammary Neoplasms Animal Mammary Neoplasms Primary Malignant Neoplasm Of Breast Infiltrating Ductal Carcinoma Of Breast Infiltrating Duct Carcinoma Of Unspecified Site Infiltrating Ductular Carcinoma Of Unspecified Site Invasive Breast Carcinoma Of No Special Type Microinvasive Carcinoma Of Breast Carcinoma With Apocrine Differentiation
Williams-Beuren Region Duplication Syndrome	Chromosome 7q11.23 Duplication Syndrome 7q11.23 Duplication Syndrome 7q11.23 Microduplication Syndrome Wbs Duplication Syndrome Somerville-Van Der Aa Syndrome Dup(7)(Q11.23) Trisomy 7q11.23 William-Beuren Region Duplication Syndrome Chromosome 7q11.23 Duplication
Fragile X Syndrome	FXS Martin-Bell Syndrome Fraxa Syndrome Marker X Syndrome X-Linked Mental Retardation And Macroorchidism Fragile X Mental Retardation Syndrome Fra Syndrome Mental Retardation, X-Linked, Associated With Marxq28 Frax Syndrome Symptomatic Form Of Fragile X Syndrome In Female Carriers Fragile-X Syndrome Fraxe Syndrome
Supravalvular Aortic Stenosis	SVAS Supravalvar Aortic Stenosis Supravalvar Aortic Stenosis, Eisenberg Type Aortic Supravalvular Stenosis Aortic Stenosis, Supravalvular Supra-Valvular Aortic Stenosis Stenosis, Aortic Supravalvular Stenosis, Supravalvular Aortic Supravalvular Stenosis, Aortic Aortic Stenosis Supravalvular

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Clinical Phase
Pre-clinical Phase

Clinical Phase

Bioactive Molecules (1)

Cat. No.	Product Name	Effect	Purity	Phase
HY-157442	GLPG3312	Inhibitor	98.63%	Phase 1

Pre-clinical Phase

Bioactive Molecules (19)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-18305	BMS-5	Inhibitor	99.84%	Unkown
HY-136848	SM1-71	Inhibitor	96.09%	Unkown
HY-18304	BMS-3	Inhibitor	99.89%	Unkown
HY-122630	TH-257	Inhibitor	98.36%	Unkown
HY-148061	DB1113		99.86%	Unkown
HY-120097	R-10015	Inhibitor	99.72%	Unkown
HY-19353	SR7826	Inhibitor	99.30%	Unkown
HY-120025	CRT0105950	Inhibitor	99.76%	Unkown
HY-151539	TH470	Inhibitor	99.62%	Unkown
HY-128062	LIMK1 inhibitor BMS-4	Inhibitor	99.1%	Unkown
HY-14227	LIMK-IN-1	Inhibitor	99.33%	Unkown
HY-175656	MDI-117740	Inhibitor	/	Unkown
HY-174229	SM311	Inhibitor	/	Unkown
HY-125444	TH251	Inhibitor	/	Unkown
HY-W150222	LIMK1-IN-3	Inhibitor	/	Unkown
HY-N10868	8β,9α-Dihydroxylindan-4(5),7(11)-dien-8alpha,12-olide	Inhibitor	/	Unkown
HY-169605	LIMK1 inhibitor 1	Inhibitor	/	Unkown
HY-12659B	LX7101 monohydrochloride	Inhibitor	/	Unkown
HY-111103	CZh226	Inhibitor	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Mus musculus	LIMK1	MGD	MGI:104572
Bos taurus	LIMK1	VGNC	VGNC:30890
Rattus norvegicus	LIMK1	RGD	RGD:62055
Canis familiaris	LIMK1	VGNC	VGNC:42681
Felis catus	LIMK1	VGNC	VGNC:68053
Macaca mulatta	LIMK1	VGNC	VGNC:74269
Others	LIMK1	NCBI