2. Metabolic Enzyme/Protease Apoptosis Stem Cell/Wnt JAK/STAT Signaling Immunology/Inflammation
  3. Endogenous Metabolite Apoptosis STAT BCL6 Interleukin Related Caspase
  4. Glycochenodeoxycholic acid sodium salt

Glycochenodeoxycholic acid sodium salt  (Synonyms: Chenodeoxycholylglycine sodium salt; Sodium glycochenodeoxycholate)

Cat. No.: HY-N2334A Purity: 98.51%
COA
SDS
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Glycochenodeoxycholic acid sodium salt (Sodium glycochenodeoxycholate) is a relatively toxic bile salt generated in the liver from chenodeoxycholic acid and glycine. Glycochenodeoxycholic acid sodium salt inhibits Autophagosome formation and impairs lysosomal function by inhibiting lysosomal proteolysis and increasing lysosomal pH in human normal liver cells, leading to the Apoptosis of human hepatocyte cells. Glycochenodeoxycholic acid sodium salt induces stemness and chemoresistance via activating STAT3 signaling pathway in hepatocellular carcinoma cells (HCC). Glycochenodeoxycholic acid sodium salt is promising for research in the field of cholestasis desease, hepatocellular carcinoma and primary sclerosing cholangitis (PSC).

For research use only. We do not sell to patients.

CAS No. : 16564-43-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

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Based on 5 publication(s) in Google Scholar

Other Forms of Glycochenodeoxycholic acid sodium salt:

Glycochenodeoxycholic acid sodium salt Related Antibodies

Top Publications Citing Use of Products

    Glycochenodeoxycholic acid sodium salt purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb 3:e2411719.  [Abstract]

    The mRNA levels of ALDOB in Huh7 or HCCLM3 cells treated with various bile acids (100 μM, 24 h). All data are presented as mean ± SD. Data were analyzed by one-way ANOVA with Bonferroni multiple-comparison correction. CA, cholic acid; TCA, taurocholic acid; GCA, glycocholic acid; TCDCA, taurochenodeoxycholic acid; GCDCA, glycochenodeoxycholic acid; LCA, lithocholic acid; TLCA, taurolithocholic acid; GLCA, glycolithocholic acid; DCA, deoxycholic acid; TDCA, taurodeoxycholic acid; GDCA, glycodeoxycholic acid; UDCA, ursodeoxycholic acid; TUDCA, tauroursodeoxycholic acid; GUDCA, glycoursodeoxycholic acid.

    Glycochenodeoxycholic acid sodium salt purchased from MedChemExpress. Usage Cited in: Biomolecules. 2025 Jun 28;15(7):943.  [Abstract]

    SARS-CoV-2-stimulated THP-1 cells were treated with 10 μM or 20 μM Glycochenodeoxycholic acid (GCDCA) for 24 h. The intracellular mRNA expression levels of IL-1β, IL-8, and CXCL5 were measured by RT–qPCR.

    View All Endogenous Metabolite Isoform Specific Products:

    View All Isoforms
    Human Endogenous Metabolite Microbial Metabolite

    View All STAT Isoform Specific Products:

    View All Isoforms
    STAT3 STAT5 STAT6 STAT1

    View All Interleukin Related Isoform Specific Products:

    View All Isoforms
    IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

    View All Caspase Isoform Specific Products:

    View All Isoforms
    Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Glycochenodeoxycholic acid sodium salt (Sodium glycochenodeoxycholate) is a relatively toxic bile salt generated in the liver from chenodeoxycholic acid and glycine. Glycochenodeoxycholic acid sodium salt inhibits Autophagosome formation and impairs lysosomal function by inhibiting lysosomal proteolysis and increasing lysosomal pH in human normal liver cells, leading to the Apoptosis of human hepatocyte cells. Glycochenodeoxycholic acid sodium salt induces stemness and chemoresistance via activating STAT3 signaling pathway in hepatocellular carcinoma cells (HCC). Glycochenodeoxycholic acid sodium salt is promising for research in the field of cholestasis desease, hepatocellular carcinoma and primary sclerosing cholangitis (PSC)[1][2][3][4].

    IC50 & Target

    Microbial Metabolite

     

    Human Endogenous Metabolite

     

    Cellular Effect
    Cell Line Type Value Description References
    CHO EC50
    3.88 3
    Compound: 4b, glyco
    Agonist activity at human TGR5 expressed in CHO cells by luciferase assay
    Agonist activity at human TGR5 expressed in CHO cells by luciferase assay
    		[PMID: 18307294]
    HET-1A CC50
    729 3
    Compound: 3, TCDCA
    Cytotoxicity against human HET-1A cells assessed as cell viability after 24 hrs by MTT assay
    Cytotoxicity against human HET-1A cells assessed as cell viability after 24 hrs by MTT assay
    		[PMID: 20713311]
    In Vitro

    Glycochenodeoxycholic acid sodium salt (0-100 μM, 6 h) significantly increases the amount of dead cells and decreases in LC3, ATG5 and BECN1 expression in human normal liver cells, leading to Autophagosome formation inhibition[1].
    Glycochenodeoxycholic acid sodium salt (5–500 μM, 24 h) exerts no induction or reduction of TGF-β mRNA expression in KMBC cells and LX-2 cells[2].
    Glycochenodeoxycholic acid sodium salt (200 μM, 24 h and 48 h) enhances stemness and chemoresistance of hepatocellular carcinoma cells (HCC) by activating the STAT3 signaling pathway, suppressing the expression of apoptotic genes (Bcl10, Caspase 3, Caspase 4, Tp53, BAD) and increasing the expression of anti-apoptotic genes (Bcl2, Bcl-xl and IL10)[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Glycochenodeoxycholic acid sodium salt Related Antibodies

    Apoptosis Analysis[4]

    Cell Line: Huh7 and LM3 cell lines
    Concentration: 200 μM
    Incubation Time: 24 h and 48 h
    Result: Increased the cell viability and promoted chemoresistance treated with 5-FU (HY-90006) (120 µg/mL) and cisplatinin (10 µg/mL) in Huh7 and LM3 cell lines

    Cell Autophagy Assay[1]

    Cell Line: L02 cells
    Concentration: 100 μM
    Incubation Time: 6 h
    Result: Significantly reduced GFP-LC3 puncta and decreased microtubule-associated protein 1 light chain 3 (LC3), autophagy related 5 (ATG5) and beclin 1 (BECN1) expression in L02 cells.

    Western Blot Analysis[1]

    Cell Line: Human normal liver cells
    Concentration: 0-100 μM
    Incubation Time: 6 h
    Result: Decreased the TFE3 levels in a dose-dependent manner in human normal liver cells.

    RT-PCR[2]

    Cell Line: KMBC cells
    Concentration: 5–500 μM
    Incubation Time: 24 h
    Result: Unaltered TGF-β mRNA expression in KMBC cells and LX-2 cells.

    Real Time qPCR[4]

    Cell Line: Huh7 and LM3 cell lines
    Concentration: 200 μM
    Incubation Time: 24 h and 48 h
    Result: Suppressed the expression of apoptotic genes (Bcl10, Caspase 3, Caspase 4, Tp53, BAD) and increased the expression of anti-apoptotic genes (Bcl2, Bcl-xl and IL10) in Huh7 and LM3 cell lines.
    Molecular Weight

    471.61

    Formula

    C26H42NNaO5
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[C@@]12[C@](CC[C@]2([H])[C@H](C)CCC(NCC(O[Na])=O)=O)([H])[C@@]3([H])[C@]([C@@]4([C@](C[C@H](O)CC4)([H])C[C@H]3O)C)([H])CC1
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 250 mg/mL (530.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : ≥ 100 mg/mL (212.04 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1204 mL 10.6020 mL 21.2040 mL
    5 mM 0.4241 mL 2.1204 mL 4.2408 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (4.41 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (4.41 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 50 mg/mL (106.02 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    (per animal)

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    Dosing volume
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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 98.51%

    SDS (393 KB)

    COA (248 KB) HNMR (274 KB) RP-HPLC (252 KB) MS (70 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 2.1204 mL 10.6020 mL 21.2040 mL 53.0099 mL
    5 mM 0.4241 mL 2.1204 mL 4.2408 mL 10.6020 mL
    10 mM 0.2120 mL 1.0602 mL 2.1204 mL 5.3010 mL
    15 mM 0.1414 mL 0.7068 mL 1.4136 mL 3.5340 mL
    20 mM 0.1060 mL 0.5301 mL 1.0602 mL 2.6505 mL
    25 mM 0.0848 mL 0.4241 mL 0.8482 mL 2.1204 mL
    30 mM 0.0707 mL 0.3534 mL 0.7068 mL 1.7670 mL
    40 mM 0.0530 mL 0.2650 mL 0.5301 mL 1.3252 mL
    50 mM 0.0424 mL 0.2120 mL 0.4241 mL 1.0602 mL
    60 mM 0.0353 mL 0.1767 mL 0.3534 mL 0.8835 mL
    80 mM 0.0265 mL 0.1325 mL 0.2650 mL 0.6626 mL
    100 mM 0.0212 mL 0.1060 mL 0.2120 mL 0.5301 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Glycochenodeoxycholic acid sodium salt Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Glycochenodeoxycholic acid16564-43-5Chenodeoxycholylglycine Sodium glycochenodeoxycholateEndogenous MetaboliteApoptosisSTATBCL6Interleukin RelatedCaspaseB-cell lymphoma 6 proteinB-cell lymphoma 5 protein (BCL-5)Zinc finger and BTB domain-containing protein 27Zinc finger protein 51ILbile salthepatocellular carcinoma cellsautophagosome inhibitionSTAT3 signaling pathwaycholestasis deseaseprimary sclerosing cholangitisInhibitorinhibitorinhibit

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