IHCH-3064
IHCH-3064 is a dual-acting compounds targeting Adenosine A2A Receptor and HDAC. IHCH-3064 exhibits potent binding to A2AR (Ki=2.2 nM) and selective inhibition of HDAC1 (IC50=80.2 nM), with good antiproliferative activity against tumor cell lines in vitro. IHCH-3064 is a tumor immunotherapeutic agent.
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- CAS. Nr.: 2420562-65-6
- Formel: C25H21N9O2
- Molecular Weight:479.49
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
- Protokoll
- Reinheit & Dokumentation
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Biologische Aktivität
Beschreibung
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CT26 | GI50 |
6.1 μM
Compound: 24e
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Antiproliferative activity against mouse CT26 cells measured after 72 hrs by CCK8 assay
Antiproliferative activity against mouse CT26 cells measured after 72 hrs by CCK8 assay
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[PMID: 34783558] |
| MC-38 | GI50 |
6.2 μM
Compound: 24e
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Antiproliferative activity against mouse MC38 cells measured after 72 hrs by CCK8 assay
Antiproliferative activity against mouse MC38 cells measured after 72 hrs by CCK8 assay
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[PMID: 34783558] |
Chemical Information
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CAS. Nr. 2420562-65-6
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Molecular Weight 479.49
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Formel C25H21N9O2
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SMILES
O=C(C1=CC=C(CCN2N=CC3=C2N=C(N)N4N=C(C5=CC=CO5)N=C34)C=C1)NC6=C(N)C=CC=C6
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
IHCH-3064 Related Classifications
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