Isozedoarondiol
Isozedoarondiol can be isolated from the rhizomes of Curcuma xanthorrhiza Roxb. Isozedoarondiol inhibits MMP-1 expression in UVB-treated human keratinocytes.
For research use only. We do not sell to patients.
- CAS No.: 108887-68-9
- Formula: C15H24O3
- Molecular Weight:252.35
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| KB | IC50 |
>50 μg/mL
Compound: 4
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Cytotoxicity against human KB cells assessed as reduction in cell viability
Cytotoxicity against human KB cells assessed as reduction in cell viability
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[PMID: 29274817] |
| KG-1a | IC50 |
>100 μg/mL
Compound: 4
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Cytotoxicity against human KG1a cells assessed as reduction in cell viability after 48 hrs by MTT assay
Cytotoxicity against human KG1a cells assessed as reduction in cell viability after 48 hrs by MTT assay
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[PMID: 29274817] |
| MCF7 | IC50 |
>50 μg/mL
Compound: 4
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Cytotoxicity against human MCF7 cells assessed as reduction in cell viability
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability
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[PMID: 29274817] |
| NCI-H187 | IC50 |
>50 μg/mL
Compound: 4
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Cytotoxicity against human NCI-H187 cells assessed as reduction in cell viability
Cytotoxicity against human NCI-H187 cells assessed as reduction in cell viability
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[PMID: 29274817] |
| RAW264.7 | IC50 |
54.2 μM
Compound: 19
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Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
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[PMID: 23755850] |
Chemical Information
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CAS No. 108887-68-9
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Molecular Weight 252.35
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Formula C15H24O3
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SMILES
C[C@]1([C@]2([H])[C@](CC1)([H])[C@](O)(CC(/C(C2)=C(C)/C)=O)C)O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Mitochondrial membrane-potential fluorescent assay
Mitochondrial membrane potential fluorescent assays estimate ΔΨm in living cells using lipophilic cationic dyes such as TMRM, TMRE, rhodamine 123, and JC-1, which accumulate in mitochondria according to membrane polarization; loss of signal after FCCP or CCCP treatment is interpreted as mitochondrial depolarization. TMRM/TMRE and rhodamine 123 are commonly used for semi-quantitative live-cell microscopy or flow cytometry, while JC-1 can report a shift from red aggregate fluorescence to green monomer fluorescence during depolarization; interpretation requires controls because dye concentration, quenching mode, cell type, dye efflux, and mitochondrial mass can affect fluorescence independently of ΔΨm.
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Mitochondrial membrane-potential and mitochondrial mass staining
Mitochondrial membrane potential staining measures the electrochemical polarization across the mitochondrial inner membrane in live cells using lipophilic cationic fluorescent probes; early rhodamine-based work showed that selective mitochondrial dye accumulation is lost when the mitochondrial transmembrane potential is dissipated. JC-1 reports mitochondrial polarization by shifting from green monomer fluorescence to red J-aggregate fluorescence as dye concentration increases within energized mitochondria; therefore, the red/green fluorescence ratio is used as a relative readout of mitochondrial membrane potential. TMRE or TMRM staining provides a single-channel relative readout because these cationic rhodamine esters accumulate in polarized mitochondria, and lower fluorescence indicates reduced mitochondrial polarization when acquisition and dye-loading conditions are controlled. Mitochondrial mass staining is commonly performed with MitoTracker Green FM or related MitoTracker dyes as
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Isozedoarondiol Related Classifications
HY-N12150 Related Classifications
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