666-15
Based on 65 publication(s) in Google Scholar
666-15 is a potent and selective CREB inhibitor with an IC50 of 81 nM. 666-15 suppresses tumor growth in a breast cancer xenograft model.
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- 純度: 99.79%
- CAS 番号: 1433286-70-4
- 分子式: C33H31Cl2N3O5
- 分子量:620.52
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保管条件:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
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Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 666-15
More- Nat Immunol. 2024 Nov;25(11):2057-2067. [Abstract]
- Cancer Res. 2024 Sep 16;84(18):2985-3003. [Abstract]
- Nat Commun. 2022 Nov 28;13(1):7323. [Abstract]
- Nat Commun. 2022 Nov 4;13(1):6648. [Abstract]
- Nat Commun. 2022 Apr 26;13(1):2256. [Abstract]
- Sci Immunol. 2026 May;11(119):eadz0348. [Abstract]
- Acta Pharm Sin B. 2023 Mar;13(3):1128-1144. [Abstract]
- J Exp Clin Cancer Res. 2021 Oct 27;40(1):340. [Abstract]
- Sci Adv. 2025 Jul 11;11(28):eadv5986. [Abstract]
- Sci Adv. 2020 Feb 19;6(8):eaaw9960. [Abstract]
- Cell Death Dis. 2023 Dec 19;14(12):844. [Abstract]
- Cell Death Dis. 2019 Jul 8;10(7):520. [Abstract]
- J Immunother Cancer. 2022 May;10(5):e003793. [Abstract]
- J Neuroinflammation. 2025 Dec 3;22(1):283. [Abstract]
- J Neuroinflammation. 2022 Oct 14;19(1):257. [Abstract]
- Acta Pharmacol Sin. 2022 Feb;43(2):457-469. [Abstract]
- Br J Anaesth. 2026 Feb 26.
- J Am Soc Nephrol. 2021 Oct;32(10):2529-2541. [Abstract]
- Proc Natl Acad Sci U S A. 2025 Oct 14;122(41):e2517132122. [Abstract]
- Diabetes. 2025 Sep 1;74(9):1547-1561. [Abstract]
- Chin Med J (Engl). 2026 Apr 23. [Abstract]
- Arterioscler Thromb Vasc Biol. 2021 Sep;41(9):2431-2451. [Abstract]
- EMBO J. 2025 Sep 1. [Abstract]
- Phytother Res. 2021 Jul;35(7):3898-3915. [Abstract]
- Stem Cell Res Ther. 2025 Jul 21;16(1):392. [Abstract]
- Cell Rep. 2026 Jun 2;45(6):117487. [Abstract]
- Cell Rep. 2025 Jun 16;44(6):115839. [Abstract]
- Cell Rep. 2023 Oct 17;42(10):113272. [Abstract]
- Br J Pharmacol. 2020 Jan;177(2):432-448. [Abstract]
- Br J Pharmacol. 2019 Aug;176(16):2962-2976. [Abstract]
- Cell Mol Neurobiol. 2019 Mar;39(2):265-286. [Abstract]
- CNS Neurosci Ther. 2023 Mar;29(3):941-956. [Abstract]
- Ecotoxicol Environ Saf. 2022 May 25;239:113682. [Abstract]
- Mol Metab. 2023 Dec:78:101811. [Abstract]
- Cells. 2026 May 29;15(11):1000. [Abstract]
- Cells. 2023 Apr 6;12(7):1098. [Abstract]
- Nutrients. 2021 Dec 28;14(1):117. [Abstract]
- Eur J Pharmacol. 2024 Feb 5:964:176288. [Abstract]
- Cell Oncol (Dordr). 2026 Jun 6. [Abstract]
- Int J Mol Sci. 2022 Oct 18;23(20):12454. [Abstract]
- Mol Ther Methods Clin Dev. 2025 Jul 17;33(3):101537. [Abstract]
- Am J Pathol. 2024 Dec;194(12):2290-2301. [Abstract]
- Int J Cancer. 2024 Jun 1;154(11):1987-1998. [Abstract]
- Sci Rep. 2019 Mar 5;9(1):3462. [Abstract]
- iScience. 2025 Jun 28;28(8):113034. [Abstract]
- Front Mol Neurosci. 2022 Mar 24;15:831151. [Abstract]
- Transl Stroke Res. 2025 Jun;16(3):625-644. [Abstract]
- FEBS J. 2024 Jan;291(1):142-157. [Abstract]
- Pest Manag Sci. 2021 Apr;77(4):1873-1885. [Abstract]
- Front Neurosci. 2021 Sep 30:15:703044. [Abstract]
- BMC Pulm Med. 2022 Apr 11;22(1):140. [Abstract]
- Theriogenology. 2026 Apr 1:254:117813. [Abstract]
- Dig Dis Sci. 2024 Aug;69(8):2856-2874. [Abstract]
- Physiol Behav. 2021 Feb 1:229:113236. [Abstract]
- J Nat Med. 2026 Jun 30.
- bioRxiv. 2026 May 4:2026.04.30.721884. [Abstract]
- bioRxiv. 2025 Dec 8:2025.12.04.691935. [Abstract]
- bioRxiv. 2025 May 29.
- University of California. 2023 Jun.
- University of Miami. 2023 May.
- Oxid Med Cell Longev. 2022 Nov 28:2022:4926678. [Abstract]
- Research Square Preprint. 2021 Dec.
- Research Square Preprint. 2021 Feb.
- Research Square Preprint. 2021 Jan.
- Research Square Preprint. 2020 Jun.
Customer Validation & Images
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IF
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Bio/Physico-chemical Assay
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Cell Proliferation/Viability Assay
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RT-PCR
生物活性
製品説明
IC50 & Target
IC50: 81 nM (CREB)[1]
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | GI50 |
0.47 μM
Compound: 3i
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Antiproliferative activity against human A549 cells assessed as inhibition of cell growth after 72 hrs by MTT assay
Antiproliferative activity against human A549 cells assessed as inhibition of cell growth after 72 hrs by MTT assay
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[PMID: 26023867] |
| HEK-293T | IC50 |
0.081 μM
Compound: 3i
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Inhibition of CREB-mediated gene transcription in human HEK293T cells transfected with pCRE-RLuc by renilla luciferase reporter assay
Inhibition of CREB-mediated gene transcription in human HEK293T cells transfected with pCRE-RLuc by renilla luciferase reporter assay
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[PMID: 26023867] |
| HEK-293T | IC50 |
5290 nM
Compound: 3i
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Inhibition of NFkappaB (unknown origin)-mediated gene transcription in human HEK293T cells transfected with an NFkappaB firefly luciferase reporter gene assessed as inhibition of TNFalpha-stimulated gene transcription pretreated for 30 mins measured after
Inhibition of NFkappaB (unknown origin)-mediated gene transcription in human HEK293T cells transfected with an NFkappaB firefly luciferase reporter gene assessed as inhibition of TNFalpha-stimulated gene transcription pretreated for 30 mins measured after
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[PMID: 26023867] |
| MCF7 | GI50 |
0.31 μM
Compound: 3i
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Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth after 72 hrs by MTT assay
Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth after 72 hrs by MTT assay
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[PMID: 26023867] |
| MDA-MB-231 | GI50 |
0.073 μM
Compound: 3i
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Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth after 72 hrs by MTT assay
Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth after 72 hrs by MTT assay
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[PMID: 26023867] |
| MDA-MB-231 | GI50 |
0.073 μM
Compound: 666-15
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Growth inhibition of human MDA-MB-231 cells incubated for 72 hrs by MTT assay
Growth inhibition of human MDA-MB-231 cells incubated for 72 hrs by MTT assay
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[PMID: 35300089] |
| MDA-MB-468 | GI50 |
0.046 μM
Compound: 3i
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Antiproliferative activity against human MDA-MB-468 cells assessed as inhibition of cell growth after 72 hrs by MTT assay
Antiproliferative activity against human MDA-MB-468 cells assessed as inhibition of cell growth after 72 hrs by MTT assay
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[PMID: 26023867] |
| MDA-MB-468 | GI50 |
0.046 μM
Compound: 666-15
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Growth inhibition of human MDA-MB-468 cells incubated for 72 hrs by MTT assay
Growth inhibition of human MDA-MB-468 cells incubated for 72 hrs by MTT assay
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[PMID: 35300089] |
体外実験
666-15 (73 nM; for 12 hours) significantly blocks the effects caused by MSN overexpression, including cell proliferation, invasion, soft agar colony formation ability, and the expression of CREB downstream genes. 666-15 inhibits MSN overexpression-induced CREB phosphorylation[2].
666-15 (1μM; pretreated 2 hour) effectively inhibits PE-induced CREB phosphorylation. 666-15 significantly decreases the protein expression of ANP and β-MHC and inhibits the activation of ER stress, including the expression of GRP78, CHOP, ATF6, and the phosphorylation of IRE1 in PE + siRNA + 666-15 group and PE + si-CTRP3 + 666-15 group[3].
666-15 potently inhibits cancer cell growth. In MDA-MB-231 and MDA-MB-468 cells, the GI50 for 666-15 is 73 and 46 nM, respectively. In A549 and MCF-7 cells, it exhibits robust activity as well with GI50 of 0.47 and 0.31 μM. 666-15 is also found to be a rather weak inhibitor of CREB-CBP interaction with IC50 of 18.27 μM. 666-15 inhibits CREB’s transcription activity in living cells independent of direct CREB or CBP binding interaction. 666-15 is very potent in inhibiting CREB’s transcription activity. 666-15 also inhibits endogenous CREB target gene expression, the transcript level of nuclear receptor related 1 protein (Nurr1/NR4A2)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:CTRL or MSN-overexpressing MDA-MB-231 cells
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Concentration:73 nM
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Incubation Time:For 12hours
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Result:Significantly blocked the cell proliferation caused by MSN overexpression.
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Cell Line:NRCMs
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Concentration:1 μM
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Incubation Time:2 hour (pretreated)
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Result:Effectively inhibited PE-induced CREB phosphorylation.
体内実験
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:1-month-old female nude mice with MDA-MB-231 or T47D cells[2]
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Dosage:10 mg/kg
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Administration:IP; once a week; for 11 weeks
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Result:Played a good role in inhibiting the growth of breast cancer.
化学情報
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CAS 番号 1433286-70-4
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性状 Solid
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分子量 620.52
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分子式 C33H31Cl2N3O5
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Color White to off-white
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SMILES
O=C(NC1=CC=C(Cl)C=C1O)C2=CC3=CC=CC=C3C=C2OCCNC(C4=C(OCCCN)C=C(C=CC=C5)C5=C4)=O.Cl
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, sealed storage, away from moisture
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)
Publications (65)
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Journal Impact Factor
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Most Recent
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Nat Immunol
2024 Nov;25(11):2057-2067. PMID: 39358444 -
Cancer Res
Oncogenic Calreticulin Induces Immune Escape by Stimulating TGF-β Expression and Regulatory T Cell Expansion in the Bone Marrow Microenvironment. [Abstract]2024 Sep 16;84(18):2985-3003. PMID: 38885318 -
Nat Commun
Secreted EMC10 is upregulated in human obesity and its neutralizing antibody prevents diet-induced obesity in mice. [Abstract]2022 Nov 28;13(1):7323. PMID: 36443308 -
Nat Commun
2022 Nov 4;13(1):6648. PMID: 36333322 -
Nat Commun
Ebola virus VP35 hijacks the PKA-CREB1 pathway for replication and pathogenesis by AKIP1 association. [Abstract]2022 Apr 26;13(1):2256. PMID: 35474062
666-15 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Apr 26;13(1):2256. [Abstract]
WT and AKIP1−/− (KO) HepG2 cells were transfected with EBOV minigenome (p0) with or without 1 μM 666-15 or 10 μM H89 for 48 h and then immunostained with anti-VP35 (green) and anti-CREB1 (red) antibodies.
666-15 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Apr 26;13(1):2256. [Abstract]
HepG2 cells were transfected with the EBOV minigenome (p0 and p1) and treated with FSK (25 μM), H89 (10 μM), or 666-15 (1 μM) for 48 h. The amounts of trVLPs were determined by luciferase activity assay.
666-15 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Apr 26;13(1):2256. [Abstract]
HepG2 cells were transfected with the EBOV minigenome (p1) for 24 h or infected with live EBOV (MOI = 0.1). Then, the cells were treated with the indicated concentration of 666-15 or T-705 as a control for 24 h (for trVLP) and 96 h (for live EBOV infection). The amount of trVLPs was determined by luciferase activity assay, the live EBOV in the supernatant was titered by TCID50 assay and the cytotoxicity of 666-15 toward HepG2 cells was determined by performing CCK-8 assay.
666-15 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Apr 26;13(1):2256. [Abstract]
WT and AKIP1−/− HepG2 cells infected with live EBOV (MOI = 1) were treated with 10 μM H89 or 1 μM 666-15 for 96 h. vRNA levels in the cells were quantified by qRT-PCR.
666-15 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Apr 26;13(1):2256. [Abstract]
HepG2 cells infected with live EBOV (MOI = 1) were treated with 1 μM 666-15. The cell culture supernatants were collected at the indicated time points, and the viral titers were quantified as TCID50 with plaque assay.
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Sci Immunol
2026 May;11(119):eadz0348. PMID: 42066062 -
Acta Pharm Sin B
G protein-coupled receptor 35 attenuates nonalcoholic steatohepatitis by reprogramming cholesterol homeostasis in hepatocytes. [Abstract]2023 Mar;13(3):1128-1144. PMID: 36970193 -
J Exp Clin Cancer Res
ZIP10 drives osteosarcoma proliferation and chemoresistance through ITGA10-mediated activation of the PI3K/AKT pathway. [Abstract]2021 Oct 27;40(1):340. PMID: 34706747 -
Sci Adv
Microglial BDNF modulates arketamine's antidepressant-like effects through cortico-accumbal pathways. [Abstract]2025 Jul 11;11(28):eadv5986. PMID: 40632846 -
Sci Adv
Interfering MSN-NONO complex-activated CREB signaling serves as a therapeutic strategy for triple-negative breast cancer. [Abstract]2020 Feb 19;6(8):eaaw9960. PMID: 32128390 -
Cell Death Dis
2023 Dec 19;14(12):844. PMID: 38114479 -
Cell Death Dis
C1q-TNF-related protein-3 attenuates pressure overload-induced cardiac hypertrophy by suppressing the p38/CREB pathway and p38-induced ER stress. [Abstract]2019 Jul 8;10(7):520. PMID: 31285424 -
J Immunother Cancer
Ccl3 enhances docetaxel chemosensitivity in breast cancer by triggering proinflammatory macrophage polarization. [Abstract]2022 May;10(5):e003793. PMID: 35613826 -
J Neuroinflammation
SIK2 mediated mitochondrial homeostasis in spinal cord injury: modulating oxidative stress and the AIM2 inflammasome via CRTC1/CREB signaling. [Abstract]2025 Dec 3;22(1):283. PMID: 41339921 -
J Neuroinflammation
2022 Oct 14;19(1):257. PMID: 36241997 -
Acta Pharmacol Sin
2022 Feb;43(2):457-469. PMID: 33850273 -
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J Am Soc Nephrol
Integrative Cistromic and Transcriptomic Analyses Identify CREB Target Genes in Cystic Renal Epithelial Cells. [Abstract]2021 Oct;32(10):2529-2541. PMID: 34162733 -
Proc Natl Acad Sci U S A
A time-gated PKA-CREB signaling circuit licenses IL-12 responsiveness and Th1 fate in CD4+ T cells. [Abstract]2025 Oct 14;122(41):e2517132122. PMID: 41052344 -
Diabetes
2025 Sep 1;74(9):1547-1561. PMID: 40526459 -
Chin Med J (Engl)
Skin-localized sympathetic nerve-keratinocyte crosstalk fuels psoriatic inflammation via neutrophil recruitment. [Abstract]2026 Apr 23. PMID: 42036893 -
Arterioscler Thromb Vasc Biol
Salt-Inducible Kinase 3 Promotes Vascular Smooth Muscle Cell Proliferation and Arterial Restenosis by Regulating AKT and PKA-CREB Signaling. [Abstract]2021 Sep;41(9):2431-2451. PMID: 34196217 -
EMBO J
GNAS/PKA signaling promotes aberrant osteochondral differentiation of Gli1+ tendon sheath progenitors. [Abstract]2025 Sep 1. PMID: 40890486 -
Phytother Res
Administration of isoliquiritigenin prevents nonalcoholic fatty liver disease through a novel IQGAP2-CREB-SIRT1 axis. [Abstract]2021 Jul;35(7):3898-3915. PMID: 33860590 -
Stem Cell Res Ther
AKT signaling upregulates BDNF expression in induced neural stem cells that interact with microglia. [Abstract]2025 Jul 21;16(1):392. PMID: 40691843 -
Cell Rep
LKB1 inactivation elicits an NNMT-mediated methyl sink and confers dependence on PRMT5 in lung cancer. [Abstract]2026 Jun 2;45(6):117487. PMID: 42234565 -
Cell Rep
2025 Jun 16;44(6):115839. PMID: 40526468 -
Cell Rep
Primary cilia control oligodendrocyte precursor cell proliferation in white matter injury via Hedgehog-independent CREB signaling. [Abstract]2023 Oct 17;42(10):113272. PMID: 37858465 -
Br J Pharmacol
FOXO1 inhibition prevents renal ischemia-reperfusion injury via cAMP-response element binding protein/PPAR-γ coactivator-1α-mediated mitochondrial biogenesis. [Abstract]2020 Jan;177(2):432-448. PMID: 31655022 -
Br J Pharmacol
Glucagon up-regulates hepatic mitochondrial pyruvate carrier 1 through cAMP-responsive element-binding protein; inhibition of hepatic gluconeogenesis by ginsenoside Rb1. [Abstract]2019 Aug;176(16):2962-2976. PMID: 31166615 -
Cell Mol Neurobiol
MALAT1 Up-Regulator Polydatin Protects Brain Microvascular Integrity and Ameliorates Stroke Through C/EBPβ/MALAT1/CREB/PGC-1α/PPARγ Pathway. [Abstract]2019 Mar;39(2):265-286. PMID: 30607811 -
CNS Neurosci Ther
Alpha-Asarone modulates kynurenine disposal in muscle and mediates resilience to stress-induced depression via PGC-1α induction. [Abstract]2023 Mar;29(3):941-956. PMID: 36575869
666-15 purchased from MedChemExpress. Usage Cited in: CNS Neurosci Ther. 2023 Mar;29(3):941-956. [Abstract]
666-15 (0.1, 1.0 µM; 72 h) results in a dramatic decrease of FN1 protein expression in 34E +EV versus 34E +R4 cells.
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Ecotoxicol Environ Saf
Gestational exposure to fluoride impairs cognition in C57 BL/6 J male offspring mice via the p-Creb1-BDNF-TrkB signaling pathway. [Abstract]2022 May 25;239:113682. PMID: 35643027 -
Mol Metab
Deletion of Ascl1 in pancreatic β-cells improves insulin secretion, promotes parasympathetic innervation, and attenuates dedifferentiation during metabolic stress. [Abstract]2023 Dec:78:101811. PMID: 37769990 -
Cells
Lim Homeobox 8 Is Essential for Beta Adrenergic Stimulation of Thermogenesis in Human Adipocytes. [Abstract]2026 May 29;15(11):1000. PMID: 42274594 -
Cells
Glucagon Promotes Gluconeogenesis through the GCGR/PKA/CREB/PGC-1α Pathway in Hepatocytes of the Japanese Flounder Paralichthys olivaceus. [Abstract]2023 Apr 6;12(7):1098. PMID: 37048171 -
Nutrients
Roseburia hominis Increases Intestinal Melatonin Level by Activating p-CREB-AANAT Pathway. [Abstract]2021 Dec 28;14(1):117. PMID: 35010992 -
Eur J Pharmacol
Obligatory role of microglia-mobilized hippocampal CREB-BDNF signaling in the prophylactic effect of β-glucan on chronic stress-induced depression-like behaviors in mice. [Abstract]2024 Feb 5:964:176288. PMID: 38142848 -
Cell Oncol (Dordr)
P2RY6+ tumor-associated macrophages as a therapeutic target: suppressing tumor growth and enhancing chemosensitivity in triple-negative breast cancer. [Abstract]2026 Jun 6. PMID: 42250059 -
Int J Mol Sci
The Corticosterone-Glucocorticoid Receptor-AP1/CREB Axis Inhibits the Luteinizing Hormone Receptor Expression in Mouse Granulosa Cells. [Abstract]2022 Oct 18;23(20):12454. PMID: 36293309 -
Mol Ther Methods Clin Dev
EF1α, rather than CMV promoter, is suitable for luciferase tag expression in target cells for in vitro cytotoxicity assays of CAR-T cells. [Abstract]2025 Jul 17;33(3):101537. PMID: 40777728 -
Am J Pathol
Lipopolysaccharide Triggers Luminal Acidification to Promote Defense Against Bacterial Infection in Vaginal Epithelium. [Abstract]2024 Dec;194(12):2290-2301. PMID: 39222908 -
Int J Cancer
GNAS mutation inhibits growth and induces phosphodiesterase 4D expression in colorectal cancer cell lines. [Abstract]2024 Jun 1;154(11):1987-1998. PMID: 38319157 -
Sci Rep
Suppression of glioblastoma by a drug cocktail reprogramming tumor cells into neuronal like cells. [Abstract]2019 Mar 5;9(1):3462. PMID: 30837577 -
iScience
Preadipocyte-induced upregulation of IGFBP5 enhances ovarian cancer tumorigenesis via CREB signaling. [Abstract]2025 Jun 28;28(8):113034. PMID: 40734678 -
Front Mol Neurosci
The cAMP Response Element- Binding Protein/Brain-Derived Neurotrophic Factor Pathway in Anterior Cingulate Cortex Regulates Neuropathic Pain and Anxiodepression Like Behaviors in Rats. [Abstract]2022 Mar 24;15:831151. PMID: 35401106 -
Transl Stroke Res
GPR39 Agonist TC-G 1008 Promoted Mitochondrial Biogenesis and Improved Antioxidative Capability via CREB/PGC-1α Pathway Following Intracerebral Hemorrhage in Mice. [Abstract]2025 Jun;16(3):625-644. PMID: 38485864 -
FEBS J
Progesterone and cAMP synergistically induce SHP2 expression via PGR and CREB1 during uterine stromal decidualization. [Abstract]2024 Jan;291(1):142-157. PMID: 37786383 -
Pest Manag Sci
Azadirachtin downregulates the expression of the CREB gene and protein in the brain and directly or indirectly affects the cognitive behavior of the Spodoptera litura fourth-instar larvae. [Abstract]2021 Apr;77(4):1873-1885. PMID: 33284470 -
Front Neurosci
GluN2A/ERK/CREB Signaling Pathway Involved in Electroacupuncture Regulating Hypothalamic-Pituitary-Adrenal Axis Hyperactivity. [Abstract]2021 Sep 30:15:703044. PMID: 34658758 -
BMC Pulm Med
Salt-inducible kinase 2 (SIK2) inhibitor ARN-3236 attenuates bleomycin-induced pulmonary fibrosis in mice. [Abstract]2022 Apr 11;22(1):140. PMID: 35410283 -
Theriogenology
LH promotes testosterone synthesis in rooster leydig cells through YAP1/ACSL4/SOAT1 pathway. [Abstract]2026 Apr 1:254:117813. PMID: 41529502 -
Dig Dis Sci
Dual-Specificity Phosphatase 4 Promotes Malignant Features in Colorectal Cancer Through Cyclic-AMP Response Element Binding Protein/Protein Kinase CAMP-Activated Catalytic Subunit Beta Activation. [Abstract]2024 Aug;69(8):2856-2874. PMID: 38824257 -
Physiol Behav
Exogenous IGF-1 alleviates depression-like behavior and hippocampal mitochondrial dysfunction in high-fat diet mice. [Abstract]2021 Feb 1:229:113236. PMID: 33137345 -
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bioRxiv
Systems modeling identifies phenotype-determining signaling pathways controlled by phosphatase PTPRJ in diverse receptor tyrosine kinase activation settings. [Abstract]2026 May 4:2026.04.30.721884. PMID: 42146690 -
bioRxiv
Targeting CREB remodels the immune microenvironment to enhance immunotherapy responses in pancreatic cancer. [Abstract]2025 Dec 8:2025.12.04.691935. PMID: 41394608 -
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Oxid Med Cell Longev
Resveratrol Ameliorates Trigeminal Neuralgia-Induced Cognitive Deficits by Regulating Neural Ultrastructural Remodelling and the CREB/BDNF Pathway in Rats. [Abstract]2022 Nov 28:2022:4926678. PMID: 36478990 -
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溶剤 & 溶解度
体外:
DMSO : 125 mg/mL (201.44 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
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The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
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μL , mix evenly;
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Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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取扱説明書 (2659 KB)
参考文献
[1]. Xie F, et al. Identification of a Potent Inhibitor of CREB-Mediated Gene Transcription with Efficacious in Vivo Anticancer Activity. J Med Chem. 2015 Jun 25;58(12):5075-87. [Content Brief]
[2]. Qin Y, et al. Interfering MSN-NONO complex-activated CREB signaling serves as a therapeutic strategy for triple-negative breast cancer. Sci Adv. 2020 Feb 19;6(8):eaaw9960. [Content Brief]
[3]. Zhang B, et al. C1q-TNF-related protein-3 attenuates pressure overload-induced cardiac hypertrophy by suppressing the p38/CREB pathway and p38-induced ER stress. Cell Death Dis. 2019 Jul 8;10(7):520. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6116 mL | 8.0578 mL | 16.1155 mL | 40.2888 mL |
| 5 mM | 0.3223 mL | 1.6116 mL | 3.2231 mL | 8.0578 mL | |
| 10 mM | 0.1612 mL | 0.8058 mL | 1.6116 mL | 4.0289 mL | |
| 15 mM | 0.1074 mL | 0.5372 mL | 1.0744 mL | 2.6859 mL | |
| 20 mM | 0.0806 mL | 0.4029 mL | 0.8058 mL | 2.0144 mL | |
| 25 mM | 0.0645 mL | 0.3223 mL | 0.6446 mL | 1.6116 mL | |
| 30 mM | 0.0537 mL | 0.2686 mL | 0.5372 mL | 1.3430 mL | |
| 40 mM | 0.0403 mL | 0.2014 mL | 0.4029 mL | 1.0072 mL | |
| 50 mM | 0.0322 mL | 0.1612 mL | 0.3223 mL | 0.8058 mL | |
| 60 mM | 0.0269 mL | 0.1343 mL | 0.2686 mL | 0.6715 mL | |
| 80 mM | 0.0201 mL | 0.1007 mL | 0.2014 mL | 0.5036 mL | |
| 100 mM | 0.0161 mL | 0.0806 mL | 0.1612 mL | 0.4029 mL |
666-15 関連分類
HY-101120 関連分類
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