Ac-WEHD-AFC
Ac-WEHD-AFC is a fluorogenic caspase-1 substrate. Ac-WEHD-AFC can measure caspase-1 fluorogenic activity and can be used for the research of tumor and inflammation.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 210344-99-3
- 分子式: C38H37F3N8O11
- 分子量:838.74
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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生物活性
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Caspase-1 |
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Caspase-1 Fluorogenic Activity Assay[1]:
1. Incubate the cells according to your normal protocol.
2. Cells are transiently transfected with caspase-1 in combination with pcDNA3.1, Myc-cIAP2, Myc-cIAP1, or Myc-XIAP and collected 24 hr later.
3. Cells were lysed in CHEGG buffer before sonicating for 15 s at 60% amplitude (for cleavage assays).
4. Lysates were incubated with 10 mM of the fluorogenic caspase-1 substrate Ac-WEHD-AFC.
5. The release of free AFC was monitored continuously for 1 hr (excitation 380 nm, emission 460 nm) in 1 min intervals and expressed as arbitrary fluorescence units per minute.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 210344-99-3
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分子量 838.74
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分子式 C38H37F3N8O11
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シーケンスの短縮
Ac-WEHD-{AFC}
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Ac-WEHD-AFC 関連分類
HY-P2617 関連分類
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