AM-0883
Based on 1 Customer Validation
AM-0883 is a TRPC6 (Transient receptor potential canonical 6) agonist (hTRPC6 EC50 = 46 nM). AM-0083 can be used for the study of cardiac and renal diseases.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 98.94%
- 分子式: C21H21ClN2O3
- 分子量:384.86
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保管条件:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
IC50 & Target
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hTRPC6 46 nM (EC50) |
体外実験
AM-0883 can activate HEK293T cells WT TRPC6 (EC50 = 45.5 nM) and HEK293T N-terminal truncation mutant TRPC6 (Δ2–72) (EC50 = 90.2 nM)[1].
AM-0883 functionally activates the channel through specific residues at the S6/pore helix junction[1].
AM-0883 shows a significantly stronger activating effect on the V867T/L868T double mutant than on the wild-type channel[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
化学情報
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性状 Solid
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分子量 384.86
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分子式 C21H21ClN2O3
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Color White to off-white
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SMILES
O=C(N1CCC2(C(C=C(Cl)C=C3)=C3NC2)CC1)[C@@H]4OC5=CC=CC=C5OC4
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
体外:
DMSO : 103 mg/mL (267.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.58 mg/mL (6.70 mM); Clear solution
This protocol yields a clear solution of ≥ 2.58 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.58 mg/mL (6.70 mM); Clear solution
This protocol yields a clear solution of ≥ 2.58 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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データシート (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Bai Y, et al. Structural basis for pharmacological modulation of the TRPC6 channel. Elife. 2020 Mar 9;9:e53311. [Content Brief]
[2]. Chubanov V, et al. Physiological functions and pharmacological targeting of transient receptor potential channels. Pharmacol Rev. 2025 Nov;77(6):100089. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5983 mL | 12.9917 mL | 25.9835 mL | 64.9587 mL |
| 5 mM | 0.5197 mL | 2.5983 mL | 5.1967 mL | 12.9917 mL | |
| 10 mM | 0.2598 mL | 1.2992 mL | 2.5983 mL | 6.4959 mL | |
| 15 mM | 0.1732 mL | 0.8661 mL | 1.7322 mL | 4.3306 mL | |
| 20 mM | 0.1299 mL | 0.6496 mL | 1.2992 mL | 3.2479 mL | |
| 25 mM | 0.1039 mL | 0.5197 mL | 1.0393 mL | 2.5983 mL | |
| 30 mM | 0.0866 mL | 0.4331 mL | 0.8661 mL | 2.1653 mL | |
| 40 mM | 0.0650 mL | 0.3248 mL | 0.6496 mL | 1.6240 mL | |
| 50 mM | 0.0520 mL | 0.2598 mL | 0.5197 mL | 1.2992 mL | |
| 60 mM | 0.0433 mL | 0.2165 mL | 0.4331 mL | 1.0826 mL | |
| 80 mM | 0.0325 mL | 0.1624 mL | 0.3248 mL | 0.8120 mL | |
| 100 mM | 0.0260 mL | 0.1299 mL | 0.2598 mL | 0.6496 mL |
AM-0883 関連分類
HY-179490 関連分類
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