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製品番号: HY-P11704

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f-MKKFRW

f-MKKFRW is a formylated MgrB signal sequence peptide and Fpr3 agonist. f-MKKFRW can be produced by bacteria. f-MKKFRW is selectively recognized by a subset of vomeronasal sensory neurons and activates Fpr3-expressing and B2m-expressing VSNs, but not NP2-expressing VSNs. f-MKKFRW activates TRPM5-dependent signaling, triggering acetylcholine release. f-MKKFRW drives innate avoidance behavior requiring Fpr3, Gαo, and Trpc2.

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分子式: C₄₄H₆₆N₁₂O₈S
分子量:923.14

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

f-MKKFRW 構造式

価格（税別）

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性
- 製品説明
- IC₅₀ & Target
- 体外実験
- 体内実験
化学情報
純度とドキュメンテーション
参考文献
Calculators
Help & FAQs
関連分類
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生物活性

製品説明

IC₅₀ & Target

^[2]

TRPM5

Endogenous Metabolite アイソフォーム固有の製品をすべて表示 :

TRP Channel アイソフォーム固有の製品をすべて表示 :

体外実験

Bacterial InfectionOthers

Bacterial Infection

f-MKKFRW (3 μM) selectively activates HEK293T cells expressing Fpr3, and MgrB-H6 bacterial supernatant containing the MgrB-derived fMet peptide elicits a strong Fpr3-dependent Ca²⁺ response^[1].

Others

f-MKKFRW (1 μM) activates Fpr3-dependent Ca²⁺responses in VSNs of mouse VNO whole mounts, with a detection threshold ≤0.1 μM and a response proportion of approximately 0.15%^[1].
f-MKKFRW (8 min) stimulates PTS in isolated mouse trachea through a chemosensory cell-dependent, TRPM5/PLCβ2/IP3R3-dependent, Atropine (HY-B1205)- and 4-DAMP-sensitive pathway^[2].
f-MKKFRW (8 min) increases the ciliary beating frequency in isolated mouse trachea from 8.7 Hz to 14.2 Hz^[2].
f-MKKFRW (50 µM) elicits transient Ca²⁺ elevation in a subset of TRPM5⁺ tracheal chemosensory cells (19/92; 21%)^[2].
f-MKKFRW (10 µM; 15 min) triggers TRPM5-dependent acetylcholine release in isolated mouse trachea, which can be detected by M3 reporter cells and HPLC^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

f-MKKFRW (50-100 μM; oronasal administration/free exploration; single exposure) activates Fpr3-expressing vomeronasal sensory neurons in vivo and drives Fpr3-dependent innate avoidance behavior in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6N and transgenic lines (B6; ΔFpr3-lacZ, cGαo, Trpc2; adult male; 8-14 weeks old for behavioral tests; adult male for in-vivo VSN activation)^[1]
Dosage:

100 μM in PBS; 100 μM final concentration in 50% FEU; 100 μM final concentration in FEU; 50 μM final concentration in FEU
Administration:

oronasal application; single exposure; free investigation/nasal contact; 10 min
Result:

In freely behaving mice, both oronasal application and free investigation at 100 μM induced Egr1 expression in approximately 50% of Fpr3-positive VSNs; this activation was not observed with control stimuli.
In behavioral tests, 100 μM elicited robust avoidance in B6 mice, ΔFpr3-lacZ+/+, cGαo+/−, and Trpc2+/− mice.
Mice still displayed avoidance at 50 μM.
Avoidance was absent in ΔFpr3-lacZ−/−, cGαo−/−, and Trpc2−/− mice.

化学情報

分子量 923.14
分子式 C₄₄H₆₆N₁₂O₈S
配列

{fMet}-Lys-Lys-Phe-Arg-Trp
シーケンスの短縮

{fMet}-KKFRW
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (291 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Bufe B, et al. Bacterial MgrB peptide activates chemoreceptor Fpr3 in mouse accessory olfactory system and drives avoidance behaviour. Nature communications. 2019 Oct 25;10(1):4889. [Content Brief]

[2]. Perniss A, et al. Chemosensory Cell-Derived Acetylcholine Drives Tracheal Mucociliary Clearance in Response to Virulence-Associated Formyl Peptides. Immunity. 2020 Apr 14;52(4):683-699.e11.

[3]. Haga-Yamanaka S, et al. From Receptors to Behavior: Molecular and Functional Logic of Sensory Coding in the Mouse Accessory Olfactory System. Chem Senses. 2026 Jun 24:bjag017.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Minimum order quantity

100 mg

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生物活性
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- IC₅₀ & Target
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化学情報
純度とドキュメンテーション
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f-MKKFRW

Endogenous Metabolite アイソフォーム固有の製品をすべて表示

TRP Channel アイソフォーム固有の製品をすべて表示