FCW34
FCW34 is a cell-permeable, selective Sialyltransferase inhibitor. FCW34 inhibits α2,3-N-linked ST3GALIII (IC50 1.74 μM) and α2,6-N-linked ST6GALI (IC50 3.6 μM), reducing N-glycoprotein sialylation. FCW34 decreases cell migration ability by inhibiting the Talin/Integrin/FAK/Paxillin and Integrin/NFκB signaling pathways. FCW34 significantly inhibits tumor growth, reduces angiogenesis, and suppresses spontaneous lung metastasis in a nude mouse model of breast cancer. FCW34 can be used for research on breast cancer.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 2086229-19-6
- 分子式: C40H57N7O11
- 分子量:811.92
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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生物活性
製品説明
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MDA-MB-231 | IC50 |
10.64 μM
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Inhibition of serum-induced migration of human MDA-MB-231 cells assessed after 16 hrs incubation by Transwell migration assay.
Inhibition of serum-induced migration of human MDA-MB-231 cells assessed after 16 hrs incubation by Transwell migration assay.
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33371679 |
体外実験
FCW34 (10-20 μM; 48 h) disrupts talin-driven integrin activation by reducing the colocalization of integrin β1 and β3 with phospho-talin-1 in MDA-MB-231 cells[1].
FCW34 inhibits integrin sialylation in MDA-MB-231 cells[1].
FCW34 (>60 μM; 48 h) does not exhibit antiproliferative effects on MDA-MB-231 cells at concentrations up to 60 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MDA-MB-231 human breast cancer cells
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Concentration:20 μM; 2-30 μM
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Incubation Time:16 h
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Result:Abrogated serum-induced cell migration in a concentration-dependent manner with an IC50 of 10.64 μM.
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Cell Line:MDA-MB-231 cells
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Concentration:20 μM
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Incubation Time:48 h
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Result:Significantly reduced colocalization of integrins β1 and β3 and phospho-talin-1 in MDA-MB-231 cells.
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Cell Line:MDA-MB-231 cells
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Concentration:>60 μM
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Incubation Time:48 h
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Result:Did not cause an inhibitory effect on cell growth (>90% survival) at tested concentrations (>60 μM).
体内実験
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Athymic nude mice (BALB/cAnN.Cg-Foxn1nu/CrlNarl, 4-5 weeks of age, female)[1]
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Dosage:10 mg/kg
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Administration:every other day
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Result:Displayed profound tumor suppression and delayed lung metastasis.
Showed a significantly smaller number of metastatic foci in the lungs and fewer metastatic nodules in adjacent lymph node sections compared to control.
Revealed a significantly smaller number of vessels surrounding tumor nodules and significantly reduced angiogenesis.
Showed significantly less accumulation of Ki-67-positive cells than in control mice.
Indicated no detrimental effects on health based on blood tests and body weight assessments.
- Scratch/Wound-Healing Migration Assay
- Human pluripotent stem cell endothelial-cell differentiation
- Vascular/Branching Fractal Analysis
- Cell migration
- Neural Crest/Neuronal Cell Migration Explant Assay
- Patient-Derived Orthotopic Xenograft (PDOX)
- Breast Cancer Modeling
- How to Choose the Right Model Animal
- Zigmond Chamber Chemotaxis Assay
- Under-Agarose Cell Migration Assay
- Transwell/Boyden Chamber Migration Assay
- Patient-Derived Xenograft (PDX)
- Endothelial Tube Formation Assay
- Tail-Vein Experimental Metastasis Xenograft
- 3D Collagen/Hydrogel Matrix Migration Assay
- Subcutaneous Cell-Line-Derived Xenograft
- Cell-Exclusion Zone Migration Assay
- Dunn/Insall Chamber Chemotaxis Assay
- Orthotopic Cell-Line Xenograft
- Endothelial Cell Migration/Angiogenic Sprouting Assay
化学情報
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CAS 番号 2086229-19-6
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分子量 811.92
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分子式 C40H57N7O11
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SMILES
O=C(O)C[C@H](NC1=CC=C([N+]([O-])=O)C2=NON=C21)C(O[C@@H]3CC[C@]4(C)[C@@]5([H])CC[C@]6(C)[C@@H]([C@@H](CCC(OCCOCCOCCN=[N+]=[N-])=O)C)CC[C@@]6([H])[C@]5([H])CC[C@]4([H])C3)=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
FCW34 関連分類
HY-189136 関連分類
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