LLC0424N
Based on 1 Customer Validation
LLC0424N is a negative control compound designed by methylating the thalidomide moiety of LLC0424 (HY-161574). LLC0424N shows does not induce NSD2 degradation and inhibit growth of acute lymphoblastic leukemia cells with NSD2 mutation. LLC0424N can be used for the research of acute lymphoblastic leukemia.
For research use only. We do not sell to patients.
- CAS No.: 3063448-59-6
- Formula: C46H43N7O9
- Molecular Weight:837.88
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All Histone Methyltransferase Isoforms
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Biological Activity
LLC0424N (0.5-2 μM; 12-24 h) does not potently degrade NSD2 protein in SEM ALL cells[1].
LLC0424N (2 μM; 8-24 h) does not downregulate NSD2 or H3K36me2 protein levels in SEM ALL and RPMI-8402 cells[1].
LLC0424N (100 nM-30 μM; 7 days) does not dose-dependently inhibit the growth of SEM ALL and RPMI-8402 cells[1].
LLC0424N (2 μM; 30 days ) does not reduce colony formation or size in RPMI-8402 ALL cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:SEM acute lymphoblastic leukemia (ALL) cells with NSD2 E1099K gain-of-function mutation
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Concentration:0.5, 2 μM
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Incubation Time:12, 24 h
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Result:Maintained NSD2 protein levels near 100% of control levels.
Chemical Information
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CAS No. 3063448-59-6
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Appearance Solid
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Molecular Weight 837.88
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Formula C46H43N7O9
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Color Light yellow to yellow
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SMILES
O=C(N1)COC2=C1C=CC(C(N(C3CC3)CC4=CC=C(C(NC5=CC=C(C(NC6CCN(C7=CC=C(C(N(C8C(N(C)C(CC8)=O)=O)C9=O)=O)C9=C7)CC6)=O)C=C5)=O)C=C4)=O)=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : ≥ 100 mg/mL (119.35 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (2.98 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1935 mL | 5.9674 mL | 11.9349 mL | 29.8372 mL |
| 5 mM | 0.2387 mL | 1.1935 mL | 2.3870 mL | 5.9674 mL | |
| 10 mM | 0.1193 mL | 0.5967 mL | 1.1935 mL | 2.9837 mL | |
| 15 mM | 0.0796 mL | 0.3978 mL | 0.7957 mL | 1.9891 mL | |
| 20 mM | 0.0597 mL | 0.2984 mL | 0.5967 mL | 1.4919 mL | |
| 25 mM | 0.0477 mL | 0.2387 mL | 0.4774 mL | 1.1935 mL | |
| 30 mM | 0.0398 mL | 0.1989 mL | 0.3978 mL | 0.9946 mL | |
| 40 mM | 0.0298 mL | 0.1492 mL | 0.2984 mL | 0.7459 mL | |
| 50 mM | 0.0239 mL | 0.1193 mL | 0.2387 mL | 0.5967 mL | |
| 60 mM | 0.0199 mL | 0.0995 mL | 0.1989 mL | 0.4973 mL | |
| 80 mM | 0.0149 mL | 0.0746 mL | 0.1492 mL | 0.3730 mL | |
| 100 mM | 0.0119 mL | 0.0597 mL | 0.1193 mL | 0.2984 mL |
LLC0424N Related Classifications
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