ML-SA5

Name: ML-SA5
Brand: MedChemExpress
SKU: HY-152182
Rating: 5.0 (4 reviews)

Cat. No.: HY-152182 Purity: 99.69%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

ML-SA5 is a potent TRPML1 cation channel agonist that activates the entire endosomal TRPML1 (ML1) current in DMD myocytes with an EC₅₀ of 285 nM and is more potent than ML-SA1. ML-SA5 has anticancer activity and can inhibit tumour growth.

For research use only. We do not sell to patients.

CAS No. : 2418670-70-7

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 4 publication(s) in Google Scholar

4 Publications Citing Use of MCE ML-SA5

Flow Cytometry

: ML-SA5 purchased from MedChemExpress. Usage Cited in: Nat Struct Mol Biol. 2025 Jun 17:10.1038/s41594-025-01581-x. [Abstract]; Live-cell imaging of WT expressing DMXL1–mNG and mScarlet–LAMP1 to mark lysosomes upon treatment with MLSA5. Images taken at 2-min intervals for 10 min are shown. c, As in a, but after pretreatment with BafA1 to block CASM.

: ML-SA5 purchased from MedChemExpress. Usage Cited in: Nat Struct Mol Biol. 2025 Jun 17:10.1038/s41594-025-01581-x. [Abstract]; Confocal images of fixed WT U2OS cells stably expressing DMXL1-mNG treated with nocodazole (top) or pre-treated with nocodazole (5 ug/ml; 1 h) and co-treated with MLSA5 (5 μM, 2 h) (bottom). Immunostaining of LAMP1 was used to mark lysosomes (magenta). Scale bar is 10 microns; inset scale bar is 5 microns. Micrographs are from a single experiment.

: ML-SA5 purchased from MedChemExpress. Usage Cited in: Nat Aging. 2024 May;4(5):647-663. [Abstract]; Representative FACS profile showing lysosomal acidity in SC^LOH treated with ML-SA1, ML-SA5 (5-25 μM) and rapamycin for 24 h.

: ML-SA5 purchased from MedChemExpress. Usage Cited in: Nat Aging. 2024 May;4(5):647-663. [Abstract]; Representative images of mRFP-eGFP-LC3 fluorescence change and lipid accumulation, as well as quantification analysis, in PALD SC samples after phagocytosis and with or without ML-SA1, ML-SA5 (5-25 μM; 48 h) and rapamycin treatment at the indicated concentrations (n = 14 ROIs in each group).

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View All TRP Channel Isoform Specific Products:

View All Isoforms

TRPA1 TRPC3 TRPC4 TRPC5 TRPC6 TRPC7 TRPM8 TRPM2 TRPM3 TRPM4 TRPM7 TRPML1 TRPML2 TRPML3 TRPV1 TRPV2 TRPV3 TRPV4 TRPV6

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	1 μM Compound: ML-SA5	Effect on TRPML1 knockout HEK293 cells Effect on TRPML1 knockout HEK293 cells	[PMID: 38141860]

In Vitro

ML-SA5(1-100 μM, 24 h) has some cell-targeting specificity and induces substantial cell death in M12 and MeWo cells, but fully preserves normal melanocytes. It also causes a loss of mitochondrial membrane potential in M12 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

ML-SA5 (i.p., 2-5 mg/kg, daily, 2 weeks) reduces muscle necrosis in MDX mice by more than 70% and reduces central nucleated fibers, suggesting that ML-SA5 can improve muscle atrophy in mdx mice in vivo by promoting myosin repair, but has no effect in ML1 knockout mice. Moreover, ML-SA5 reduces skeletal and cardiac muscle damage in mdx mice through ML1 upregulation^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

457.99

Formula

C₁₉H₂₄ClN₃O₄S₂

CAS No.

2418670-70-7

Appearance

Solid

Color

White to light brown

SMILES

O=S(NC1=C(N2CCCCC2)C(Cl)=CC=C1)(C3=CC=C(S(=O)(N(C)C)=O)C=C3)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (218.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1835 mL	10.9173 mL	21.8345 mL
5 mM	0.4367 mL	2.1835 mL	4.3669 mL
10 mM	0.2183 mL	1.0917 mL	2.1835 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.54 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.54 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.69%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (255 KB) HNMR (263 KB) RP-HPLC (246 KB) MS (178 KB)

Handling Instructions (2659 KB)

References

[1]. Wanlu Du, et al. Lysosomal Zn2+ release triggers rapid, mitochondria-mediated, non-apoptotic cell death in metastatic melanoma. Cell Rep. 2021 Oct 19;37(3):109848. [Content Brief]

[2]. Lu Yu, et al. Small-molecule activation of lysosomal TRP channels ameliorates Duchenne muscular dystrophy in mouse models. Sci Adv. 2020 Feb 7;6(6):eaaz2736. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1835 mL	10.9173 mL	21.8345 mL	54.5863 mL
	5 mM	0.4367 mL	2.1835 mL	4.3669 mL	10.9173 mL
	10 mM	0.2183 mL	1.0917 mL	2.1835 mL	5.4586 mL
	15 mM	0.1456 mL	0.7278 mL	1.4556 mL	3.6391 mL
	20 mM	0.1092 mL	0.5459 mL	1.0917 mL	2.7293 mL
	25 mM	0.0873 mL	0.4367 mL	0.8734 mL	2.1835 mL
	30 mM	0.0728 mL	0.3639 mL	0.7278 mL	1.8195 mL
	40 mM	0.0546 mL	0.2729 mL	0.5459 mL	1.3647 mL
	50 mM	0.0437 mL	0.2183 mL	0.4367 mL	1.0917 mL
	60 mM	0.0364 mL	0.1820 mL	0.3639 mL	0.9098 mL
	80 mM	0.0273 mL	0.1365 mL	0.2729 mL	0.6823 mL
	100 mM	0.0218 mL	0.1092 mL	0.2183 mL	0.5459 mL

ML-SA5 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ML-SA52418670-70-7TRP ChannelTransient receptor potential channelsTRPML1ML1DMD myocytesanticancerML-SA1Inhibitorinhibitorinhibit

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ML-SA5

Customer Review

ML-SA5 Related Antibodies

4 Publications Citing Use of MCE ML-SA5

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All TRP Channel Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

ML-SA5 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

ML-SA5 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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