2. Apoptosis Epigenetics Cell Cycle/DNA Damage
  3. MDM-2/p53 PARP
  4. MMRi6

MMRi6 is a Mdm2-MdmX RING domain inhibitor that can disrupt Mdm2-MdmX RING-RING interaction in vitro. MMRi6 inhibits MdmX-stimulated Mdm2 autoubiquitination and Mdm2-MdmX-mediated p53 polyubiquitination in vitro without affecting NEDD4-1 autoubiquitination. MMRi6 induces p53 stabilization and accumulation and induces PARP cleavage in wt-p53 Emu-myc lymphoma cells. MMRi6 inhibits the growth of wt-p53 and p53-null Emu-myc lymphoma cells with IC50s of approximately 0.5 μM and 3 μM, respectively. MMRi6 can be used for the study of leukemia/lymphoma.

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MMRi6

MMRi6 Chemical Structure

CAS No. : 709009-15-4

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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MMRi6 Related Antibodies

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PARP PARP1 PARP2 PARP3 PARP4 TNKS1/PARP5A TNKS2/PARP5B PARP7 PARP10 PARP14 PARP15 PARP12 PARP16

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Description

MMRi6 is a Mdm2-MdmX RING domain inhibitor that can disrupt Mdm2-MdmX RING-RING interaction in vitro. MMRi6 inhibits MdmX-stimulated Mdm2 autoubiquitination and Mdm2-MdmX-mediated p53 polyubiquitination in vitro without affecting NEDD4-1 autoubiquitination. MMRi6 induces p53 stabilization and accumulation and induces PARP cleavage in wt-p53 Emu-myc lymphoma cells. MMRi6 inhibits the growth of wt-p53 and p53-null Emu-myc lymphoma cells with IC50s of approximately 0.5 μM and 3 μM, respectively. MMRi6 can be used for the study of leukemia/lymphoma[1].

In Vitro

MMRi6 (10 μM) inhibits Mdm2 autoubiquitination and p53 ubiquitination by Mdm2–MdmX in vitro[1].
MMRi6 (5 μM, 8 h) induces p53 protein accumulation in HCT-8 cells and in wt-p53 Emu-myc lymphoma cells[1].
MMRi6 (0.5 μM, 24 h) induces PARP cleavage in wt-p53 Emu-myc lymphoma cells but not in p53-null Emu-myc lymphoma cells[1].
MMRi6 (0.5-1 μM, 72 h) inhibits the growth of wt-p53 and p53-null Emu-myc lymphoma cells with IC50s of approximately 0.5 μM and 3 μM, respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MMRi6 Related Antibodies

Western Blot Analysis[1]

Cell Line: HCT-8 cells, wt-p53 Emu-myc lymphoma cells and p53-null Emu-myc lymphoma cells
Concentration: 0.5, 5, 10 μM
Incubation Time: 8, 24 h
Result: Induced p53 protein accumulation in HCT-8 cells and in wt-p53 Emu-myc lymphoma cells.
Induced PARP cleavage in wt-p53 Emu-myc lymphoma cells but not in p53-null Emu-myc lymphoma cells.
Molecular Weight

396.27

Formula

C21H15Cl2N3O
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

OC1=C2N=CC=CC2=C(Cl)C=C1C(C3=CC=CC(Cl)=C3)NC4=NC=CC=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (126.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5235 mL 12.6177 mL 25.2353 mL
5 mM 0.5047 mL 2.5235 mL 5.0471 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5235 mL 12.6177 mL 25.2353 mL 63.0883 mL
5 mM 0.5047 mL 2.5235 mL 5.0471 mL 12.6177 mL
10 mM 0.2524 mL 1.2618 mL 2.5235 mL 6.3088 mL
15 mM 0.1682 mL 0.8412 mL 1.6824 mL 4.2059 mL
20 mM 0.1262 mL 0.6309 mL 1.2618 mL 3.1544 mL
25 mM 0.1009 mL 0.5047 mL 1.0094 mL 2.5235 mL
30 mM 0.0841 mL 0.4206 mL 0.8412 mL 2.1029 mL
40 mM 0.0631 mL 0.3154 mL 0.6309 mL 1.5772 mL
50 mM 0.0505 mL 0.2524 mL 0.5047 mL 1.2618 mL
60 mM 0.0421 mL 0.2103 mL 0.4206 mL 1.0515 mL
80 mM 0.0315 mL 0.1577 mL 0.3154 mL 0.7886 mL
100 mM 0.0252 mL 0.1262 mL 0.2524 mL 0.6309 mL
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MMRi6 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MMRi6709009-15-4MMRi 6MMRi-6MDM-2/p53PARPpoly ADP ribose polymeraseleukemia/lymphomap53Mdm2-MdmX RINGInhibitorinhibitorinhibit

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