1. Anti-infection
    Autophagy
  2. Filovirus
    Parasite
    Autophagy
  3. Retro-2

Retro-2 

Cat. No.: HY-122571 Purity: ≥98.0%
Handling Instructions

Retro-2 is a selective inhibitor of retrograde protein trafficking at the endosome-trans-Golgi network interface. Retro-2 is an ebolavirus (EBOV) infection inhibitor with an EC50 of 12.2 µM in HeLa cells. Retro-2 induces cell autophagy.

For research use only. We do not sell to patients.

Retro-2 Chemical Structure

Retro-2 Chemical Structure

CAS No. : 1201652-50-7

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5 mg USD 200 In-stock
Estimated Time of Arrival: December 31
10 mg USD 350 In-stock
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25 mg USD 650 In-stock
Estimated Time of Arrival: December 31
50 mg USD 950 In-stock
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100 mg USD 1350 In-stock
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Description

Retro-2 is a selective inhibitor of retrograde protein trafficking at the endosome-trans-Golgi network interface. Retro-2 is an ebolavirus (EBOV) infection inhibitor with an EC50 of 12.2 µM in HeLa cells. Retro-2 induces cell autophagy[1][2][3].

IC50 & Target

EC50: 12.2 µM (Ebolavirus)[3]

In Vitro

Retro-2 (1 μM; 1-4 hours) induces autophagy in GFP-LC3-expressing HeLa cells and promoted the dramatic cytoplasmic accumulation of large autophagosomes[2].
Retro-2 (1 μM; 0.5-4 hours) treatment hows an increase over time of the abundance of LC3-II protein in cells[2].
Retro-2 impairs the trafficking between autophagosomes and lysosomes. Retro-2 abolishes autolysosomes formation[2].
Retro-2 (20 μM; pretreatment for 30 min) inhibits HeLa cell intoxication by ricin, Shiga-like toxins 1 (Stx1) and 2 (Stx2)[1].
Retro-2 blocked invasion of cells by the intracellular parasite Leishmania, as well as replication of non-enveloped viruses, including polyoma-, papilloma-, and adeno-associated viruses[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Autophagy Assay[2]

Cell Line: HeLa cells
Concentration: 1 μM
Incubation Time: 1 hour, 2 hours, 4 hours
Result: Resulted in a large significant increase in the number of small and large vesicles.

Western Blot Analysis[2]

Cell Line: HeLa cells
Concentration: 1 μM
Incubation Time: 0.5 hour, 2 hours, 4 hours
Result: Showed an increase over time of the abundance of LC3-II protein in cells.
In Vivo

Retro-2 (2-200 mg/kg; i.p; daily; for 21 days) treatment clearly protects from lethal nasal exposure to ricin in mice. 200 mg/kg of Retro-2 fully protects mice against ricin challenge[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female Balb/c mice (Pathogen-free 6-week-old) injected with ricin (2 μg/kg)[1]
Dosage: 2 mg/kg, 10 mg/kg, 20 mg/kg, 200 mg/kg
Administration: Intraperitoneal injection; daily; for 21 days
Result: Protected mice against ricin challenge.
Molecular Weight

320.41

Formula

C₁₉H₁₆N₂OS

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (780.25 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1210 mL 15.6050 mL 31.2100 mL
5 mM 0.6242 mL 3.1210 mL 6.2420 mL
10 mM 0.3121 mL 1.5605 mL 3.1210 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.08 mg/mL (6.49 mM); Suspended solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.08 mg/mL (6.49 mM); Suspended solution; Need ultrasonic

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (6.49 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Retro-2
Cat. No.:
HY-122571
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