Cat. No.: HY-N2081

Skimmianine

4 Cited Publications

Customer Review

Based on 4 publication(s) in Google Scholar

Skimmianine is an orally active furoquiniline alkaloid present mainly in the Rutaceae family. Skimmianine has analgesic, antispastic, sedative, and anti-inflammatory properties. Skimmianine inhibits acetylcholinesterase (AChE) (IC₅₀ = 8.6 μg/mL). Skimmianine exhibits cytotoxicity against a variety of cancer cell lines and genotoxicity. Skimmianine has antioxidant and anti-inflammatory effects on ischemia-reperfusion (IR) injury. Skimmianine exerts anti-inflammatory effects through activation of the phosphatidylinositol-3-kinase (PI3K)-protein kinase B (AKT) pathway. Skimmianine is neuroprotective by targeting the NF-κB activation pathway to prevent neuroinflammation. Skimmianine inhibits the release of histamine, intracellular Ca²⁺ signaling and protein kinase C signaling.

For research use only. We do not sell to patients.

Purity: 99.90%
CAS No.: 83-95-4
Formula: C₁₄H₁₃NO₄
Molecular Weight:259.26

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Skimmianine Chemical Structure

CAS No.: 83-95-4

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[4]

AChE

8.6 μg/mL (IC₅₀)

All Cholinesterase (ChE) Isoforms :

AChE
BChE

All PI3K Isoforms :

All Akt Isoforms :

All NF-κB Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	4.2 μg/mL Compound: 7	Cytotoxicity against human A2780 cells Cytotoxicity against human A2780 cells	[PMID: 12713408]
A549	IC₅₀	>10 μM Compound: 8	Cytotoxicity against human A549 cells by SRB assay Cytotoxicity against human A549 cells by SRB assay	[PMID: 18950230]
Neutrophil	IC₅₀	13.24 μM Compound: 18	Antiinflammatory activity in human neutrophils assessed as inhibition of formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B-induced superoxide anion generation Antiinflammatory activity in human neutrophils assessed as inhibition of formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B-induced superoxide anion generation	[PMID: 18211005]
Neutrophil	IC₅₀	19.15 μM Compound: 18	Antiinflammatory activity in human neutrophils assessed as inhibition of formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B-induced elastase release Antiinflammatory activity in human neutrophils assessed as inhibition of formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B-induced elastase release	[PMID: 18211005]
Neutrophil	IC₅₀	35.5 μM Compound: 8	Immunomodulatory activity in polymorphoneutrophils assessed as inhibition of luminol-induced oxidative burst by chemiluminescence assay Immunomodulatory activity in polymorphoneutrophils assessed as inhibition of luminol-induced oxidative burst by chemiluminescence assay	[PMID: 18950230]

In Vitro

Skimmianine (Compound 1) (1 μg) has remarkable AChE inhibitory activity in alkaloidal extract of Z. nitidum^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Skimmianine (20 mg/kg, p.o., single dose) results in addition reaction of oxygen in metabolites of male SD rats^[1].
Skimmianine (40 mg/kg, p.o., single dose) leads to a reduction in MDA levels and an increase in SOD and CAT activities in female Wistar albino rats^[2].
Skimmianine (1-10 mg/kg, i.p., single dose) results in 82% edema inhibition with 5 mg/kg dose where 5 mg/kg is the minimal concentration for maximal edema inhibition in male Wistar rats^[3].
Skimmianine (5 mg/kg, i.p., single dose) leads to a significant decrease in the concentration of PGE₂ compared to the Carrageenan (Carr) (HY-125474) and a non-significant decrease compared to the Diclofenac (Dic) (HY-15036) group of male Wistar rats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

24 female Wistar albino rats^[2]
Dosage:

40 mg/kg
Administration:

Oral gavage (p.o.) single dose
Result:

Significantly downregulated the expression of IBA-1 in cerebral tissues.
Downregulated the expression of IL-6 in cells in both white and gray matter.
Prevented the activation of NF-κB in neurons and glial cells, lowering NF-κB expression.
Significantly decreases NF-κB immune reactivity in cerebral tissues.

Animal Model:

Adult male Wistar rats (140-160 g)^[3]
Dosage:

5 mg/kg
Administration:

Intraperitoneal injection (i.p.) single dose
Result:

Showed a significant decrease when Skimmianine used as a pre-treatment.
Showed a significant decrease in the level of TBARS when combined with Carr compared to Carr alone.
Showed a significant reduction in TNF-α and IL-6 mRNA levels when combined with Carr.

Chemical Information

CAS No. 83-95-4
Appearance Solid
Molecular Weight 259.26
Formula C₁₄H₁₃NO₄
Color White to off-white
SMILES

COC1=C2N=C(OC=C3)C3=C(OC)C2=CC=C1OC
Structure Classification
- Alkaloids
- Quinoline Alkaloids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (4)

All

Sort by:

Journal Impact Factor

Antioxidants (Basel)

Skimmianine Pretreatment Attenuates Cerebellar Neuroinflammation and Myelin Injury Following Experimental Cerebral Ischemia-Reperfusion. [Abstract]

2026 Jun 11;15(6):743. PMID: 42352049
Pharmaceuticals (Basel)

Skimmianine Modulates Tumor Proliferation and Immune Dynamics in Breast Cancer by Targeting PCNA and TNF-α. [Abstract]

2025 May 20;18(5):756. PMID: 40430573
Curr Issues Mol Biol

Skimmianine Showed Neuroprotection against Cerebral Ischemia/Reperfusion Injury. [Abstract]

2024 Jul 12;46(7):7373-7385. PMID: 39057078
Research Square Print

2023 Mar 9.

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (128.56 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.8571 mL

19.2857 mL

38.5713 mL

5 mM

0.7714 mL

3.8571 mL

7.7143 mL

10 mM

0.3857 mL

1.9286 mL

3.8571 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Huang A, et al. Metabolic Profile of Skimmianine in Rats Determined by Ultra-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Tandem Mass Spectrometry. Molecules. 2017 Mar 23;22(4). [Content Brief]

[2]. Ayaz, H., et al., (2024). Skimmianine Showed Neuroprotection against Cerebral Ischemia/Reperfusion Injury. Current issues in molecular biology, 46(7), 7373–7385. [Content Brief]

[3]. Ratheesh, M., et al., (2013). Anti-inflammatory effect of quinoline alkaloid skimmianine isolated from Ruta graveolens L. Inflammation research : official journal of the European Histamine Research Society ... [et al.], 62(4), 367–376. [Content Brief]

[4]. Yang, Z.-d., Zhang, D.-b., Ren, J., et al. (2011) Skimmianine, a furoquinoline alkaloid from Zanthoxylum nitidum as a potential acetylcholinesterase inhibitor. Med. Chem. Res. 21, 722-725.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.8571 mL	19.2857 mL	38.5713 mL	96.4283 mL
	5 mM	0.7714 mL	3.8571 mL	7.7143 mL	19.2857 mL
	10 mM	0.3857 mL	1.9286 mL	3.8571 mL	9.6428 mL
	15 mM	0.2571 mL	1.2857 mL	2.5714 mL	6.4286 mL
	20 mM	0.1929 mL	0.9643 mL	1.9286 mL	4.8214 mL
	25 mM	0.1543 mL	0.7714 mL	1.5429 mL	3.8571 mL
	30 mM	0.1286 mL	0.6429 mL	1.2857 mL	3.2143 mL
	40 mM	0.0964 mL	0.4821 mL	0.9643 mL	2.4107 mL
	50 mM	0.0771 mL	0.3857 mL	0.7714 mL	1.9286 mL
	60 mM	0.0643 mL	0.3214 mL	0.6429 mL	1.6071 mL
	80 mM	0.0482 mL	0.2411 mL	0.4821 mL	1.2054 mL
	100 mM	0.0386 mL	0.1929 mL	0.3857 mL	0.9643 mL

Keywords

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Skimmianine

Immunology/Inflammation Compound Library

Neuronal Signaling Compound Library

NF-κB Signaling Compound Library

PI3K/Akt/mTOR Compound Library

Stem Cell Signaling Compound Library

Natural Product Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Oxygen Sensing Compound Library

Medicine Food Homology Compound Library

Glycolysis Compound Library

Pyroptosis Compound Library

Cytoskeleton Compound Library

Orally Active Compound Library

Glutamine Metabolism Compound Library

Traditional Chinese Medicine Active Compound Library

Neuroprotective Compound Library

Alkaloids Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Angiogenesis-Related Compound Library

Transcription Factor-Targeted Library

Glucose Metabolism Compound Library

Anti-Cancer Natural Product Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Human Metabolite Library

Anti-Pulmonary Fibrosis Compound Library

Heterocyclic Compound Library

Pain-Related Compound Library

Membrane Protein-targeted Compound Library

Multi-Target Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Anti-Fibrosis Compound Library

Miao Ethnicity Medicine Compound Library

Tibetan Medicine Compound Library

PBCRBS Traditional Chinese Medicine Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

RO5 Drug-like Natural Product Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Mass Spectrometry Human Metabolite Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Mongolian Medicine Compound Library

Chinese Baijiu Compound Library

Tonifying Traditional Chinese Medicine Monomer Compound Library

Lactylation Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Skimmianine

All Cholinesterase (ChE) Isoforms

All PI3K Isoforms

All Akt Isoforms

All NF-κB Isoforms

Biological Activity

Chemical Information

Publications (4)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Skimmianine Related Classifications

HY-N2081 Related Classifications